The University of Arizona
Associate Professor
The University of Arizona
Assistant Professor
University of California, San Diego 2010 - 2011
Assistant Project Scientist
University of California, San Diego 2005 - 2010
Postdoctoral Fellow
Skills:
Biochemistry Protein Expression Molecular Biology Cell Biology Protein Purification Protein Chemistry Confocal Microscopy Cell Culture Western Blotting Scientific Writing Fluorescence Genetics Immunoprecipitation Proteomics Microscopy Tissue Culture Pcr Flow Cytometry Cell Assay Development Mass Spectrometry Fluorescence Microscopy Drug Discovery Molecular Cloning
An intramolecular bioluminescence resonance energy transfer (BRET), biosensor for monitoring receptor activity and signalling cascades is disclosed. The “double-brilliance” biosensor sandwiches β-arrestin (β-arr) between Renilla (Luc) and the yellow fluorescent protein (YFP). β-arr associates with G-protein coupled receptors GPCR following receptor activation, bringing Luc and YPF into close proximity that favours energy transfer. In addition to providing new insights into the agonist-induced conformational rearrangements of β-arr in living cells, the double-brilliance β-arr offers a universal biosensor for GPCR activation, allowing the study of native receptors in large-scale screening analysis. The activity of other signalling molecules known to interact with β arrestin could also be monitored by double brilliance β arr.
Michel Bouvier - Montreal, CA Pascale Charest - San Diego CA, US Christian Le Gouill - Montreal, CA Alexandre Beautrait - Montreal, CA
International Classification:
C12N 9/96 C07K 19/00 C07K 17/02
US Classification:
435188, 530402
Abstract:
The present invention relates to a novel biosensor. A resonance energy transfter (RET) biosensor comprising a beta(β)-arrestin tagged with a first and a second chromophore, wherein said first chromophore is a fluorophore and said second chromophore is a fluorophore or a bioluminophore is described.
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Pascale Charest
Youtube
Dmo Pascale Dudemaine MtoMdia
Category:
Science & Technology
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01 Sep, 2010
Duration:
3m 16s
THE CROW-E: Multi-Tool
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David Usher - Everyday Things (Acoustic) feat...
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Belle : Multiple French Cast Version
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Category:
Entertainment
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Category:
Film & Animation
Uploaded:
20 Dec, 2007
Duration:
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Notre-Dame de Paris (2006)
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Category:
Entertainment
Uploaded:
03 Feb, 2008
Duration:
6m 26s
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