Patricia D Doyle

US Patent:

8420054, Apr 16, 2013

Filed:

Sep 18, 2009

Appl. No.:

12/562757

Inventors:

James Robert Schwartz - West Chester OH, US
Kathleen Marie Kerr - Okeana OH, US
Patricia West Doyle - Cincinnati OH, US
Kenneth Robert Wehmeyer - Cincinnati OH, US
Thomas Massey Burt - Middletown OH, US
Angela Marie Fieno - Hamilton OH, US
Rohan Lalith Wimalasena - Mason OH, US
Gina Marie Fadayel - Maineville OH, US

Assignee:

The Procter & Gamble Company - Cincinnati OH

International Classification:

A61K 49/00

US Classification:

424 91, 424 92, 424 98

Abstract:

A method for measuring of histamine in an epidermis comprising applying an adhesive article to an epithelium of a mammal; allowing for adherence of epithelial cells to the adhesive article; removing the adhesive article from the epithelium of the mammal; preparing the adhesive article using standard laboratory methods for extraction; extracting histamine from the epithelial cells adhered to said adhesive article; measuring histamine from the epithelial cells adhered to said adhesive article; determining the amount of histamine in the epithelial cells as compared to a baseline sample. Further, a method of objectively measuring the perception of itch in mammals and wherein there is a reduction in histamine from a baseline level which is directly proportional to a reduction in an itch perception.