Patricia Jo Keely

US Patent:

7805183, Sep 28, 2010

Filed:

Jun 21, 2007

Appl. No.:

11/766321

Inventors:

Patricia Jo Keely - Madison WI, US
Paolo P. Provenzano - Madison WI, US
John Graham White - Madison WI, US
Kevin William Eliceiri - Madison WI, US

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

A61B 6/00
G01J 3/00

US Classification:

600476, 356300

Abstract:

The present invention provides methods and systems for evaluating biological materials for the diagnosis of disease, such as gland abnormalities, and the cancerous and precancerous conditions. Nonlinear optical microscopy techniques, such as MP microscopy and harmonic generation microscopy, are used to generate high resolution, three dimensional images of a test tissue, such as a biopsy tissue sample and tissue in whole organisms, that are analyzed, optionally in combination, to detect, identify and characterize tumor-associated collagen signatures. The presence, abundance and extent of histological features and structural motifs comprising tumor-associated collagen signatures may be directly and accurately correlated with the onset and progression of cancer, such as breast cancer. The present methods are capable of providing an accurate and selective diagnosis of cancer, and provide diagnostic information complementary to conventional diagnostic methods.

US Patent:

8144966, Mar 27, 2012

Filed:

Feb 29, 2008

Appl. No.:

12/040013

Inventors:

Paolo P. Provenzano - Madison WI, US
Patricia Jo Keely - Madison WI, US
Kevin W. Eliceiri - Madison WI, US
John G. White - Madison WI, US

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

A61B 5/00
G06K 9/00

US Classification:

382133, 600477

Abstract:

The present invention broadly provides methods and systems for detecting, identifying, and characterizing conditions, including diseases and other disorders in human or other animal subjects, by analyzing fluorescence from endogenous flavin adenine dinucleotide (FAD) fluorophors present in biological materials and samples. In particular embodiments, the invention relates to conditions of the human breast including cancers such as carcinoma. Methods and systems are provided for detecting, locating, and characterizing tumors and precancerous tissue via nonlinear optical imaging techniques capable of accurately characterizing fluorescence intensities and fluorescent lifetime parameters from endogenous FAD fluorophors present in a test tissue sample.