Ms. Reynolds works in Williamsburg, VA and specializes in Cardiovascular Disease. Ms. Reynolds is affiliated with Sentara Williamsburg Regional Medical Center.
Charles L. Hershberger - New Palestine IN Jeffrey L. Larson - Indianapolis IN Patricia A. Reynolds - Indianapolis IN
Assignee:
Eli Lilly and Company - Indianapolis IN
International Classification:
C12N 120 C12N 1500 C07H 2104
US Classification:
4352523
Abstract:
The present invention discloses an. about. 1. 4 kb Bc1I-Sau3A restriction fragment containing the minimal replicon of the Streptomyces coelicolor plasmid SCP2*. This minimal replicon was deduced from plasmid derivatives constructed by in vitro deletions and has been identified as the smallest, self-replicating segment of the SCP2* plasmid. The minimal replicon specifies an ultrahigh level of plasmid DNA (more than 1000 fold increase from the basic replicon) when vectors containing this sequence are transformed into Streptomyces lividans. This replicon can be used to construct smaller, more efficient recombinant DNA shuttle vectors to clone DNA into streptomycetes.
McWilliams has been on death row for more than thirty years. His guilt regarding the 1984 rape and murder of Patricia Reynolds was not much debated eyewitnesses saw him at the scene of the crime, and he was caught driving a stolen car with the murder weapon.
In Utica, N.Y., Patricia Reynolds has struggled to find treatment after becoming dependent on hydrocodone pills originally prescribed for a broken tailbone. She said the nearest clinics offering the rehabilitation programs she wants are full and not accepting new patients.