Bruce Ivins - Frederick MD Patricia Worsham - Jefferson MD Arthur M. Friedlander - Gaithersburg MD Joseph W. Farchaus - Frederick MD Susan L. Welkos - Frederick MD
Assignee:
The United States of America as represented by the Secretary of the Army - Washington DC
A method of making a vaccine for anthracis that inolves a bacterial expression system and production and use of protective antigen (PA) against. The PA immunogen is useful in a vaccine against human anthrax. The PA can be produced by an asporogenic organism which produces the desired antigen, which is then harvested from the supernatant.
Patricia Worsham - Jefferson MD Arthur M. Friedlander - Gaithersburg MD Bruce Ivins - Frederick MD
Assignee:
The United States of America as represented by the Secretary of the Army - Washington DC
International Classification:
C12N 1500
US Classification:
4242461
Abstract:
This invention relates to a bacterial expression system for production of protective antigen (PA) against bacillus anthracis. Recombinant asporogenic B. anthracits that are derived from. DELTA. Sterne-1(pPA102) and show inability to bind the dye when grown on Congo Red Agar can be screened and asporogenic strains isolated using methods of the invention. organisms of the invention lacking spore-forming function may be killed by heat shock at temperatures as low as 60. degree. C. for 60 minutes. Hence, contamination of the environment with viable spore-forming organisms is easily avoided and decontamination is easily accomplished.
them that "DNA sequencing should show the differences in genetics," the mutations that make morphs grow differently. Ivins had good reason to understand the biology of morphs: One of his best friends at the lab, Patricia Worsham, had published a pioneering paper on the subject several years earlier.