Patrick J Macdonald

US Patent:

20200209250, Jul 2, 2020

Filed:

Dec 27, 2019

Appl. No.:

16/729067

Inventors:

- Abbott Park IL, US
Patrick J. Macdonald - Abbott Park IL, US
Kerry M. Swift - Abbott Park US, US
Sergey Y. Tetin - Abbott Park IL, US
Brenda Calfin - Abbott Park IL, US
Zhen Lin - Abbott Park IL, US
Richard Haack - Abbott Park IL, US
Mark R. Pope - Abbott Park IL, US
John Prostko - Abbott Park IL, US
Xiaoxing Qiu - Abbott Park IL, US
Felicia Bogdan - Abbott Park IL, US

International Classification:

G01N 33/58
G01N 15/14
G01N 33/543

Abstract:

The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a cross-linked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

US Patent:

20190376963, Dec 12, 2019

Filed:

May 3, 2019

Appl. No.:

16/403165

Inventors:

- Abbott Park IL, US
Jeffrey B. Huff - Abbott Park IL, US
Joseph P. Skinner - Abbott Park IL, US
Patrick MacDonald - Abbott Park IL, US
Qiaoqiao Ruan - Abbott Park IL, US

International Classification:

G01N 33/543
G01N 33/58

Abstract:

The disclosure provides a method for an enhanced detection of an analyte present in a biological sample. After the formation of the analyte/specific binding member(s)/detectable label complex, the labels are eluted and a first aliquot of eluant is brought into contact with a solid support, wherein the solid support comprises immobilized thereto specific binding member that specifically binds to the label, removing the first aliquot from the solid support and contacting the solid support with a second aliquot of the eluted label, and repeating the above steps, such that the label is concentrated on the solid support for further analysis to quantify the analyte in the biological sample.