Patrick J Sheridan

US Patent:

20010026918, Oct 4, 2001

Filed:

Dec 28, 2000

Appl. No.:

09/752213

Inventors:

Mark Collins - Walnut Creek CA, US
Thomas Horn - Berkeley CA, US
Patrick Sheridan - San Leandro CA, US
Brian Warner - Martinez CA, US
Michael Urdea - Alamo CA, US

International Classification:

C12Q001/68
C07H021/02
C07H021/04
C12P019/34
C07K001/00
A23J001/00
C07K014/00
C07K016/00
C07K017/00

US Classification:

435/006000, 536/024500, 530/413000, 435/091200

Abstract:

Methods are provided for substantially reducing background signals encountered in nucleic acid hybridization assays. The method is premised on the elimination or significant reduction of the phenomenon of nonspecific hybridization, so as to provide a detectable signal which is produced only in the presence the target polynucleotide of interest. In addition, a novel method for the chemical synthesis of isoguanosine or 2′-deoxy-isoguanosine is provided. The invention also has applications in antisense and aptamer therapeutics and drug discovery.

US Patent:

57472440, May 5, 1998

Filed:

Dec 23, 1991

Appl. No.:

7/813338

Inventors:

Patrick Sheridan - San Leandro CA
Joyce Running - Concord CA
Michael S. Urdea - Alamo CA

Assignee:

Chiron Corporation - Emeryville CA

International Classification:

C12Q 168
C07H 2104
C07K 1708
C08L 8900

US Classification:

435 6

Abstract:

Nucleic acid probes are immobilized on polystyrene surfaces such as the wells of microtiter plates for use in solution phase nucleic acid sandwich hybridization assays, particularly those using large branched DNA amplification multimers, by: (a) cleansing the surface by washing it sequentially with a strong acid, a strong base, and water, (b) passively adsorbing a polypeptide having primary amino groups onto the cleansed surface, and (c) covalently bonding the probe to the adsorbed polypeptide via a base-stable bifunctional crosslinking agent, and (d) subjecting the surface to conditions that simulate the hybridization conditions used in the assay.

US Patent:

57806101, Jul 14, 1998

Filed:

Feb 3, 1997

Appl. No.:

8/794153

Inventors:

Mark L. Collins - Walnut Creek CA
Thomas Horn - Berkeley CA
Patrick J. Sheridan - San Leandro CA
Brian D. Warner - Martinez CA
Michael S. Urdea - Alamo CA

International Classification:

C07H 2104
C07H 2102
C12Q 168
C12P 1934

US Classification:

536 245

Abstract:

Methods are provided for substantially reducing background signals encountered in nucleic acid hybridization assays. The method is premised on the elimination or significant reduction of the phenomenon of nonspecific hybridization, so as to provide a detectable signal which is produced only in the presence the target polynucleotide of interest. In addition, a novel method for the chemical synthesis of isoguanosine or 2'-deoxy-isoguanosine is provided. The invention also has applications in antisense and aptamer therapeutics and drug discovery.

US Patent:

50494902, Sep 17, 1991

Filed:

Feb 20, 1990

Appl. No.:

7/482137

Inventors:

John W. H. Sutherland - Rochester NY
Patrick J. Sheridan - San Leandro CA
Louis M. Mezei - Fremont CA

Assignee:

Eastman Kodak Co. - Rochester NY
Cetus Corporation - Emeryville CA

International Classification:

C12Q 168
C07H 1512
C12N 1500

US Classification:

435 6

Abstract:

A convenient method for the quantitative determination of a DNA polymerase includes contacting an aqueous test specimen suspected of containing the enzyme with the following: a single-stranded DNA template present in a concentration of at least about 10. sup. -8 molar bases, a DNA primer complementary to the template, a source of a metal polymerase cofactor, sufficient deoxyribonucleoside triphosphates to synthesize double-stranded DNA in the presence of the polymerase, and a colorimetric or fluorescent dye which is capable of providing a detectable signal when a primed single-stranded nucleic acid is converted to double-stranded DNA by the polymerase. The rate of signal generation is then measured and can be correlated with the level of DNA polymerase in the specimen using graphical or mathematical means. The results of this method are precise, having a covariance of less than about 10%. A test kit includes the reagents needed for carrying out this method.

US Patent:

57802270, Jul 14, 1998

Filed:

Jun 7, 1995

Appl. No.:

8/472756

Inventors:

Patrick J. Sheridan - San Leandro CA
Julio C. Gagne - Emeryville CA
Mary L. Anderson - ALamo CA

International Classification:

C12Q 168
C07H 2104
C07H 2102

US Classification:

435 6

Abstract:

A method of preparing a homogeneous alkaline phosphatase-oligonucleotide probe conjugate having high specific enzyme activity for use in nucleic acid hybridization assays is disclosed. Indirect, competitive nucleic acid hybridization assay formats are also described.

US Patent:

62324624, May 15, 2001

Filed:

Jul 14, 1998

Appl. No.:

9/115566

Inventors:

Mark L. Collins - Walnut Creek CA
Thomas Horn - Berkeley CA
Patrick J Sheridan - San Leandro CA
Brian D. Warner - Martinez CA
Michael S. Urdea - Alamo CA

Assignee:

Bayer Corporation - East Walpole MA

International Classification:

C07H 2100
C07H 2104
C07H 2102

US Classification:

536 253

Abstract:

Methods are provided for substantially reducing background signals encountered in nucleic acid hybridization assays. The method is premised on the elimination or significant reduction of the phenomenon of nonspecific hybridization, so as to provide a detectable signal which is produced only in the presence the target polynucleotide of interest. In addition, a novel method for the chemical synthesis of isoguanosine or 2'-deoxy-isoguanosine is provided. The invention also has applications in antisense and aptamer therapeutics and drug discovery.

US Patent:

58539743, Dec 29, 1998

Filed:

Mar 5, 1996

Appl. No.:

8/610955

Inventors:

Patrick J. Sheridan - San Leandro CA

Assignee:

Chiron Corporation - Emeryville CA

International Classification:

C12Q 100
C12Q 168
C12Q 142
C12N 900

US Classification:

435 4

Abstract:

Methods and compositions for enhancing the chemiluminescence from a stable 1,2-dioxetane triggered to produce a chemiluminescence are disclosed. Indirect, competitive nucleic acid hybridization assay formats are also described that employ these methods and compositions.

US Patent:

47896302, Dec 6, 1988

Filed:

Aug 20, 1986

Appl. No.:

6/896677

Inventors:

Will Bloch - El Cerrito CA
Patrick J. Sheridan - San Leandro CA
Robert J. Goodson - Albany CA

Assignee:

Cetus Corporation - Emeryville CA

International Classification:

G01N 3353

US Classification:

435 7

Abstract:

Useful for visualizing biological materials in a solid phase, on a gel, or in a liquid phase is a solid salt of the meriquinone of benzidine or a substituted benzidine. An immobilized or dissolved complex of a polymeric anion and the meriquinone of benzidine or a substituted benzidine having controllable solubility may also be employed. Preferred are meriquinone salts and complexes of 3,3,5,5'-tetramethylbenzidine. For visualization, the benzidine or substituted benzidine is oxidized to its meriquinone at pH 3 to 7 in the presence of an effective anion or polymeric anion, an oxidation catalyst, and an effective amount of oxidant to form a solid salt or immobilized complex of the meriquinone under conditions where the meriquinone solubility lies below about 10. sup. -5 M.