Paul R Atkinson

US Patent:

55653308, Oct 15, 1996

Filed:

May 19, 1995

Appl. No.:

8/445308

Inventors:

Paul R. Atkinson - Indianapolis IN
Matthew D. Hilton - Indianapolis IN
Peter K. Lambooy - Indianapolis IN

Assignee:

Eli Lilly and Company - Indianapolis IN

International Classification:

C12P 2106
C12N 948
C12N 958

US Classification:

435 681

Abstract:

A method for removing dipeptides from the amino terminus of precursor polypeptides to produce a polypeptide product is presented which comprises contacting the precursor polypeptide for sufficient time to remove the dipeptide with a dipeptidylaminopeptidase (dDAP) from the slime mold, Dictyostelium descoideum, which has a mass of about 225 kilodaltons and a pH optimum of about 3. 5. The precursor polypeptides may be made recombinantly and may be analogs of naturally occurring polypeptides.

US Patent:

58405178, Nov 24, 1998

Filed:

Mar 24, 1997

Appl. No.:

8/823104

Inventors:

Paul Robert Atkinson - Indianapolis IN
Lisa Kay Foster - Greenwood IN
Thomas Charles Furman - Indianapolis IN
Warren Cameron MacKellar - Plainfield IN

Assignee:

Eli Lilly and Company - Indianapolis IN

International Classification:

C12P 2102

US Classification:

435 681

Abstract:

The present invention is directed to an improved process for preparing in high yield an obesity protein analog using a dipeptidylaminopeptidase isolated from the slime mold, Dictyostelium discoideum.

US Patent:

55653499, Oct 15, 1996

Filed:

Sep 7, 1994

Appl. No.:

8/301519

Inventors:

Paul R. Atkinson - Indianapolis IN
Matthew D. Hilton - Indianapolis IN
Peter K. Lambooy - Indianapolis IN

Assignee:

Eli Lilly and Company - Indianapolis IN

International Classification:

C12N 958
C12N 948

US Classification:

435223

Abstract:

A dipeptidylaminopeptidase (dDAP enzyme) and a method for isolating it from the culture medium of the cellular slime mold, Dictyostelium discoideum have been presented. The isolated dDAP enzyme has a pH optimum of about 3. 5 and a mass of about 225 kDA. The dDAP enzyme has an activity which is somewhat similar to both DAP-I and DAP-III from this organism. Methods for using the dDAP enzyme to remove dipeptides from the N-terminus of recombinantly produced precursor proteins or peptides are also presented.

US Patent:

55739237, Nov 12, 1996

Filed:

Dec 22, 1993

Appl. No.:

8/172501

Inventors:

Paul R. Atkinson - Indianapolis IN
Lisa K. Foster - Greenwood IN

Assignee:

Eli Lilly and Company - Indianapolis IN

International Classification:

C12P 2106
C12N 948
C12N 958

US Classification:

435 681

Abstract:

A method for removing dipeptides from the amino terminus of precursor polypeptides to produce a polypeptide product is presented which comprises contacting the precursor polypeptide for sufficient time to remove the dipeptide with an immobilized dipeptidylaminopeptidase (dDAP) from the slime mold, Dictyostelium discoideum, which has a mass of about 225 kilodaltons and a pH optimum of about 3. 5. The precursor polypeptides may be made recombinantly and may be analogs of naturally occurring polypeptides.

US Patent:

44383657, Mar 20, 1984

Filed:

Feb 16, 1982

Appl. No.:

6/348640

Inventors:

Paul J. Atkinson - Buffalo Grove IL

Assignee:

Zenith Radio Corporation - Glenview IL

International Classification:

H02H 104
H02H 906

US Classification:

313325

Abstract:

An improved spark gap device and configuration for coupling the device in circuit with equipment such as television receivers are disclosed. The spark gap device includes three conductive pads, the first and second of which are separated by a first gap. The third pad is separated from the other pads by a second gap which intersects the first gap. By coupling the first and second pads across A. C. power lines in the equipment, and by coupling the third pad to earth ground, the energy associated with a high voltage transient on the power lines causes the air in one of the gaps to ionize. Hence, a spark jumps the gap to shunt the transient energy to ground. The intersection of the gaps causes ionization to spread to the second gap and thereby provide an additional path to ground for the energy associated with the transient, thereby protecting components within the equipment.