Paul A Atkinson

US Patent:

48618640, Aug 29, 1989

Filed:

Jun 13, 1988

Appl. No.:

7/206916

Inventors:

Paul H. Atkinson - New York NY
A. Richard Bellamy - Auckland, NZ
Gerald W. Both - Northride, AU
Marianne S. Poruchynsky - Bronx NY
Chiara Tyndall - Mosman, AU

Assignee:

Albert Einstein College of Medicine of Yeshiva University (a Div. of
Yeshiva University) - Bronx NY

International Classification:

C07K 710
C07K 1300

US Classification:

530324

Abstract:

A soluble form of the neutralizing antigen or rotavirus is provided. The antigen is formed by cloning DNA coding for VP7 protein into an expression vector and preparing deletion mutants coding for a soluble cell secreted neutralizing antigen. The preparation of the deletion mutants includes the removal of the DNA sequences coding for those amino acids of the VP7 protein responsible for its normal intracellular location and non-secretion characteristics. The deletion mutant may be used in the formation of stably transformed animal cell lines which contiuously secrete the antigen, or in the production of certain yeast strains which produce the soluble antigen with appropriate glycosylation.