Paul Knutsen Barstad Sandvik (9 April 1847 24 December 1936) was a Norwegian educator. He was born in rsta as a son of Knut Barstad and his wife Ragnhild,
Us Patents
Method Of Determining Thiopurine Methyltransferase Activity
The present invention provides a method of determining thiopurine methyltransferase (TPMT) activity in a subject. The method includes the steps of reacting sample obtained from the subject with a thiopurine derivative that is not 6-mercaptopurine to produce a methylated purine product; contacting the reacted sample with acid, thereby precipitating proteinaceous material from the reacted sample; separating supernatant from the precipitated proteinaceous material; and detecting in the supernatant the methylated purine product, where the amount of the methylated purine product indicates a level of thiopurine methyltransferase activity in the subject. In a method of the invention, the subject can be, for example, an inflammatory bowel disease patient. In one embodiment, the acid used to precipitate proteinaceous material is perchloric acid, for example, 70% perchloric acid. In another embodiment, the thiopurine derivative used as a substrate is 6-thioguanine.
Method Of Determining Thiopurine Methyltransferase Activity
The present invention provides a method of determining thiopurine methyltransferase (TPMT) activity in a subject. The method includes the steps of reacting sample obtained from the subject with a thiopurine derivative that is not 6-mercaptopurine to produce a methylated purine product; contacting the reacted sample with acid, thereby precipitating proteinaceous material from the reacted sample; separating supernatant from the precipitated proteinaceous material; and detecting in the supernatant the methylated purine product, where the amount of the methylated purine product indicates a level of thiopurine methyltransferase activity in the subject. In a method of the invention, the subject can be, for example, an inflammatory bowel disease patient. In one embodiment, the acid used to precipitate proteinaceous material is perchloric acid, for example, 70% perchloric acid. In another embodiment, the thiopurine derivative used as a substrate is 6-thioguanine.
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Paul Barstad
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Single
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Love animals and have 6 cats. I know don't say it! Maybe more the little girl outside has created a litter somewhere. When I find them they will be up for adoption, just give me a call :).
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Old timer, 38 years with the same company, a utility in Phoenix, Az.
Bragging Rights:
Three wonderful kids that make me proud, Mark, Terri, Ryan. Oh did I mention 6 cats?