Paul Dr Price

US Patent:

8198084, Jun 12, 2012

Filed:

Jun 14, 2005

Appl. No.:

11/151647

Inventors:

Stephen F. Gorfien - Williamsville NY, US
Richard M. Fike - Clarence NY, US
Glenn P. Godwin - North Tonawanda NY, US
Joyce L. Dzimian - Depew NY, US
David A. Epstein - East Amherst NY, US
Dale Gruber - East Amherst NY, US
Don McClure - Indianapolis IN, US
Paul J. Price - Grand Island NY, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C12N 5/00
C12N 5/02
C12N 5/07
C12N 5/10
C12N 11/00
C12N 11/16

US Classification:

435358, 435174, 435383, 435389, 435404

Abstract:

The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.

US Patent:

8455246, Jun 4, 2013

Filed:

May 8, 2009

Appl. No.:

12/463351

Inventors:

Stephen Gorfien - Williamsville NY, US
Richard Fike - Clarence NY, US
Glenn Godwin - North Tonawanda NY, US
Joyce Dzimian - Lancaster NY, US
David A. Epstein - East Amherst NY, US
Dale Gruber - East Amherst NY, US
Paul Price - Grand Island NY, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

A61K 33/32
A61K 33/34
A01N 59/16
C12N 5/00
C12N 5/02
C12N 5/07
C12N 5/10
C12N 11/00
C12N 11/16

US Classification:

435325, 424641, 435174, 435358, 435383, 435389, 435404

Abstract:

The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.

US Patent:

20020076747, Jun 20, 2002

Filed:

Sep 7, 1999

Appl. No.:

09/390634

Inventors:

PAUL J. PRICE - GRAND ISLAND NY, US
MINDY D. GOLDSBOROUGH - GAITHERSBURG MD, US
MARY LYNN TILKINS - NIAGARA FALLS NY, US

International Classification:

C12P021/02
C07H021/04
C12N005/02
C12N005/06

US Classification:

435/069100, 435/325000, 435/374000, 435/375000, 435/377000, 435/395000, 435/404000, 435/405000, 435/406000, 435/407000, 435/455000, 536/023100

Abstract:

The present invention provides a serum-free supplement which supports the growth of embryonic stem cells in culture. Also provided are a medium comprising a basal medium supplemented with the serum-free supplement of the present invention. The present invention also provides methods for culturing and isolating embryonic stems cells, methods for producing a transgenic animal, and methods for expressing recombinant protein in embryonic stem cells and transgenic animals.

US Patent:

20040171152, Sep 2, 2004

Filed:

Jan 26, 2004

Appl. No.:

10/763252

Inventors:

Paul Price - Grand Island NY, US
Steve Gorfien - Williamsville NY, US
Douglas Danner - Wilson NY, US
Mark Plavsic - Williamsville NY, US

Assignee:

Invitrogen Corporation

International Classification:

C12N005/02

US Classification:

435/404000

Abstract:

The present invention provides serum-free cell culture media formulations which are capable of supporting the in vitro cultivation of animal cells. The media comprise at least one nutrient of non-animal derivation, such as at least one plant peptide and/or at least one non-animal or plant lipid and/or fatty acid. The media may further optionally comprise an enzymatic digest or extract of yeast cells. The present invention also provides methods of cultivating animal cells in vitro using these cell culture media formulations. In addition, the media of the present invention can be used for growth of animal cells for virus production.

US Patent:

20060286668, Dec 21, 2006

Filed:

Jun 12, 2006

Appl. No.:

11/450877

Inventors:

Paul Price - Grand Island NY, US
Steve Gorfien - Williamsville NY, US
Douglas Danner - Wilson NY, US
Mark Plavsic - Williamsville NY, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12N 5/06
C12N 5/02

US Classification:

435325000, 435404000

Abstract:

The present invention provides serum-free cell culture media formulations which are capable of supporting the in vitro cultivation or animal cells. The media comprise at least one nutrient of non-animal derivation, such as at least one plant peptide and/or at least one non-animal or plant lipid and/or fatty acid. The media may further optionally comprise an enzymatic digest or extract of yeast cells. The present invention also provides methods of cultivating animal cells in vitro using these cell culture media formulations. In addition, the media of the present invention can be used for growth of animal cells for virus production.

US Patent:

20090061516, Mar 5, 2009

Filed:

Sep 11, 2008

Appl. No.:

12/209078

Inventors:

Paul Price - Grand Island NY, US
Steven Gorfien - Williamsville NY, US
Douglas Danner - Wilson NY, US
Mark Plavsic - Williamsville NY, US

Assignee:

INVITROGEN CORPORATION - Carlsbad CA

International Classification:

C12N 5/06

US Classification:

435383, 435404

Abstract:

The present invention provides serum-free cell culture media formulations which are capable of supporting the in vitro cultivation of animal cells. The media comprise at least one nutrient of non-animal derivation, such as at least one plant peptide and/or at least one non-animal or plant lipid and/or fatty acid. The media may further optionally comprise an enzymatic digest or extract of yeast cells. The present invention also provides methods of cultivating animal cells in vitro using these cell culture media formulations. In addition, the media of tile present invention can be used for growth of animal cells for virus production.

US Patent:

20090280533, Nov 12, 2009

Filed:

May 8, 2009

Appl. No.:

12/463371

Inventors:

Stephen F. Gorfien - Williamsville NY, US
Richard M. Fike - Clarence NY, US
Glenn P. Godwin - North Tonawanda NY, US
Joyce L. Dzimian - Lancaster NY, US
David A. Epstein - East Amherst NY, US
Dale Gruber - East Amherst NY, US
Don McClure - Indianapolis IN, US
Paul J. Price - Grand Island NY, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12P 21/00

US Classification:

435 691

Abstract:

The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.

US Patent:

20110039330, Feb 17, 2011

Filed:

Apr 14, 2010

Appl. No.:

12/760014

Inventors:

Paul PRICE - Grand Island NY, US
Steve Gorfien - Williamsville NY, US
Douglas Danner - Wilson NY, US
Mark Plavsic - Williamsville NY, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12N 5/02
C12N 5/07
C12N 5/071
C12N 5/16
C12N 5/073
C12N 5/09
C12N 5/078

US Classification:

435346, 435387, 435348, 435349, 435390, 435366, 435391, 435358, 435365, 435367, 435352, 435363, 435369, 435372, 435355

Abstract:

The present invention provides serum-free cell culture media formulations which are capable of supporting the in vitro cultivation of animal cells. The media comprise at least one nutrient of non-animal derivation, such as at least one plant peptide and/or at least one non-animal or plant lipid and/or fatty acid. The media may further optionally comprise an enzymatic digest or extract of yeast cells. The present invention also provides methods of cultivating animal cells in vitro using these cell culture media formulations. In addition, the media of the present invention can be used for growth of animal cells for virus production.