Peter L George

US Patent:

20210164038, Jun 3, 2021

Filed:

Jul 25, 2019

Appl. No.:

17/263108

Inventors:

- Cambridge MA, US
Haluk TEZCAN - Cambridge MA, US
John SPINOSA - Cambridge MA, US
Alexander ROBERTSON - Cambridge MA, US
Rohith SRIVAS - Cambridge MA, US
Neil PETERMAN - Cambridge MA, US
Nicole LAMBERT - Cambridge MA, US
Peter GEORGE - Cambridge MA, US
Ram YALAMANCHILI - Cambridge MA, US
Kenneth NESMITH - Cambridge MA, US

International Classification:

C12Q 1/6869
C12Q 1/6806

Abstract:

The present disclosure provides methods and systems for nucleic acid sequencing. Such systems and methods may use a single flow cell to perform unbiased and/or biased sequencing to generate libraries of nucleic acid molecules. An aspect of the present disclosure provides a method for increasing complexity of a sample for sequencing, the method comprising: providing a first nucleic acid sample having a first degree of complexity that differs from a desired degree of complexity; providing a second nucleic acid sample having a second degree of complexity that differs from the first degree of complexity and that differs from the desired degree of complexity; pooling at least a portion of the first nucleic acid sample and at least a portion of the second nucleic acid sample, thereby generating a pooled nucleic acid sample having the desired degree of complexity; and sequencing at least a portion of the pooled nucleic acid sample.