Peter A Levine

US Patent:

6387366, May 14, 2002

Filed:

Dec 31, 1998

Appl. No.:

09/224048

Inventors:

David R. Hurwitz - Boston MA
Van Cherington - Harvard MA
Theofanis Galanopoulos - Arlington MA
Peter H. Levine - Worcester MA
Joel S. Greenberger - Sewickley PA

Assignee:

ALG Company - Marlboro MA

International Classification:

A01N 6300

US Classification:

424 931, 424 932, 4241301, 424 9321, 514 44, 435325

Abstract:

The methods of the present invention are based on the discovery that adverse side effects (such as hemorrhage) can occur upon infusion of cells that express tissue factor. Accordingly, the methods of the invention are aimed at reducing the biological activity of tissue factor (TF) in a patient, and can be carried out by, for example: infusing fewer cells (or infuse the same number of BMSCs over a longer period of time); reducing the expression or activity of TF (within the infused cells specifically (e. g. , by contacting the cells with a TF antagonist in vitro) or within the patient generally (e. g. , by administering a TF antagonist to the patient); hampering the interaction of TF with factor VII(a); inhibiting the activity of the TF-factor VII(a) complex once it has formed; or inhibiting the coagulation cascade at any point downstream from formation of the complex (including inhibition of platelet activation).

US Patent:

20100300899, Dec 2, 2010

Filed:

Jun 21, 2010

Appl. No.:

12/819720

Inventors:

Peter M. Levine - Hamden CT, US
Kenneth L. Shepard - Ossining NY, US
Ping Gong - Elmhurst NY, US

Assignee:

THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK - New York NY

International Classification:

G01N 27/26
G01N 27/28
H01L 21/30

US Classification:

205792, 20440301, 438 49, 257E21211

Abstract:

Electrochemical sensing of biomolecules eliminates the need for bulky optical instruments required in traditional fluorescence-based sensing assays. Integration of the sensor interface electrodes and active electrochemical detection circuitry on CMOS substrates miniaturizes the sensing platform, enhancing portability for point-of-care applications, while enabling high-throughput, highly-parallel analysis. One embodiment includes a four-by-four active sensor array for multiplexed electrochemical biomolecular detection in a standard 0.25-μm CMOS process. Integrated potentiostats, including control amplifiers and dual-slope ADCs, stimulate the electrochemical cell and detect the current flowing through on-chip gold electrodes at each sensor site resulting from biomolecular reactions occurring on the chip surface. Post-processing techniques for fabricating biologically-compatible surface-electrode arrays in CMOS that can withstand operation in harsh electrochemical environments are described. Demonstrations showing example operation of the active CMOS array for biomolecular detection include cyclic voltammetry of a reversible redox species, DNA probe density characterization, and quantitative and specific DNA hybridization detection in real time.

US Patent:

58492879, Dec 15, 1998

Filed:

Mar 22, 1995

Appl. No.:

8/408536

Inventors:

Joel S. Greenberger - Lincoln MA
Peter H. Levine - Worcester MA

Assignee:

Stromagene Corporation - Lincoln MA
Gene Therapy Sciences, Inc. - Worcester MA

International Classification:

A61K 4800
C12N 510

US Classification:

424 9321

Abstract:

A method of causing production and secretion into the bloodsream of a human patient of a biologically active enzyme for which the human patient suffers a deficiency; the method involves introducing into the human patient donor bone marrow stromal cells which have been transfected with a gene encoding the enzyme, so that the introduced cells can adhere to a bone cavity surface of the patient and produce and secrete the active enzyme.

US Patent:

59938013, Nov 30, 1999

Filed:

Aug 19, 1997

Appl. No.:

8/914631

Inventors:

Joel S. Greenberger - Lincoln MA
Peter H. Levine - Worcester MA

Assignee:

ALG Company - Marlboro MA

International Classification:

A01N 6300

US Classification:

424 9321

Abstract:

A method of causing production and secretion into the bloodstream of a human patient of a biologically active enzyme for which the human patient suffers a deficiency; the method involves introducing into the human patient donor bone marrow stromal cells which have been transfected with a gene encoding the enzyme, so that the introduced cells can adhere to a bone cavity surface of the patient and produce and secrete the active enzyme.