Qi Chen

US Patent:

7915011, Mar 29, 2011

Filed:

Oct 8, 2009

Appl. No.:

12/575550

Inventors:

Qi Chen - Wallingford PA, US
Qiong Cheng - Wilmington DE, US
Kevin Michael Croker - Hockessin DE, US
Thomas Eduard Friedmann - Hockessin DE, US
Kristin Ruebling-Jass - Wilmington DE, US

Assignee:

E.I. du Pont de Nemours and Company - Wilmington DE

International Classification:

C12P 21/00
C12N 1/21

US Classification:

435 691, 43525233

Abstract:

Disrupting the expression of endogenous host cell genes gcvA and spr provides mutant host cells having increased heterologous peptide production. The addition of a genetic modification to the coding region of gene yejM further enhances peptide production and facilitates easier downstream processing. Recombinant host cells are provided as well as methods of using such host cells for heterologous peptide production.

US Patent:

8288137, Oct 16, 2012

Filed:

Nov 15, 2010

Appl. No.:

12/946120

Inventors:

Qi Chen - Wallingford PA, US
Qiong Cheng - Wilmington DE, US
Andrew C. Eliot - Wilmington DE, US
Kristin Ruebling-Jass - Wilmington DE, US

Assignee:

E I du Pont de Nemours and Company - Wilmington DE

International Classification:

C12N 1/21

US Classification:

435146, 43525233, 435159

Abstract:

Recombinant () bacteria that have enhanced acetyl-CoA synthetase activity and the ability to produce glycerol and glycerol-derived products, such as 3-hydroxypropionic acid, methylglyoxal, 1,2-propanediol, and 1,3-propanediol, are described. The recombinant comprise a promoter operably linked to a nucleotide sequence encoding a polypeptide having acetyl-CoA synthetase enzyme activity, wherein the promoter and nucleotide sequence are each independently either native or non-native.

US Patent:

8629243, Jan 14, 2014

Filed:

Aug 16, 2011

Appl. No.:

13/210488

Inventors:

Qi Chen - Wallingford PA, US
Qiong Cheng - Wilmington DE, US
Jian Ping Lai - Wallingford PA, US
Kristin Ruebling-Jass - Wilmington DE, US

Assignee:

E I du Pont de Nemours and Company - Wilmington DE

International Classification:

C07K 14/245

US Classification:

530350, 536 237

Abstract:

Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria are described. Additionally, variant or recombinant bacteria comprising these variant sucrose transporter polypeptides, and methods of utilizing the bacteria to produce products such as glycerol and glycerol-derived products are provided.

US Patent:

20120214202, Aug 23, 2012

Filed:

Jun 21, 2011

Appl. No.:

13/164990

Inventors:

Ritu Bhalla - New Delhi, IN
Qi Chen - Wallingford PA, US
Qiong Cheng - Wilmington DE, US
Neeraj Pandey - Haryana, IN
Pierre E. Rouviere - Wilmington DE, US
Kristin Ruebling-Jass - Wilmington DE, US
Annapurna Sachan - Kanpur, IN

Assignee:

E. I. DU PONT DE NEMOURS AND COMPANY - Wilmington DE

International Classification:

C12P 21/06
C12N 1/21

US Classification:

435 691, 43525233

Abstract:

Methods are provided to obtain recombinant microbial cells having at least one genetic modification that increase the buoyant density of a recombinant microbial cell or the buoyant density of inclusion bodies produced within a recombinant microbial cell. Exemplified are genetic modifications that increase the buoyant density of a recombinant microbial cell expressing heterologous peptides and polypeptides. Increasing expression of the genes ysaB, glyQ, glyS or a combination thereof within the recombinant microbial cell produces cells or inclusion bodies having higher buoyant density. A similar effect was achieved by decreasing or disrupting expression of the endogenous gltA gene. Increases in buoyant density render peptide production more efficient with respect to time and costs.

US Patent:

20130045519, Feb 21, 2013

Filed:

Aug 16, 2011

Appl. No.:

13/210550

Inventors:

Qi Chen - Wallingford PA, US
Qiong Cheng - Wilmington DE, US
Jian Ping Lai - Wallingford PA, US
Kristin Ruebling-Jass - Wilmington DE, US

Assignee:

E. I. DU PONT DE NEMOURS AND COMPANY - Wilmington DE

International Classification:

C12P 7/42
C12P 7/20
C12P 7/18
C12N 1/21

US Classification:

435146, 4352523, 43525233, 435159, 435158

Abstract:

Recombinant bacteria having an improved ability to utilize sucrose are provided. These recombinant bacteria have nucleotide sequences encoding sucrose utilization polypeptides integrated into their genome between the yihP gene or its homolog and the yihO gene or its homolog. Additionally, methods of utilizing the recombinant bacteria to produce products such as glycerol and glycerol-derived products are provided.