Ramon L Gonzalez

Address:

29613 Allegro Dr, Wesley Chapel, FL 33543

Phone:

8139075356

VIN:

JM1BK32F291223735

Make:

MAZDA

Model:

MAZDA3

Year:

2009

US Patent:

20220251613, Aug 11, 2022

Filed:

Apr 5, 2022

Appl. No.:

17/713480

Inventors:

Ramon GONZALEZ - Tampa FL, US
James M. CLOMBURG - Tampa FL, US

International Classification:

C12P 7/6409

Abstract:

Methods of using microorganisms to make chemicals and fuels, including carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives are described. Native or engineered thiolases are used condense a growing acyl-ACP and acetyl-ACP in combination with type II fatty acid synthesis. The resulting fatty acid biosynthesis cycle has an ATP yield analogous to the functional reverse β-oxidation cycle.

US Patent:

20210054427, Feb 25, 2021

Filed:

Jul 15, 2020

Appl. No.:

16/930220

Inventors:

Ramon Gonzalez - Tampa FL, US
Seokjung CHEONG - Houston TX, US
James M. CLOMBURG - Houston TX, US

International Classification:

C12P 17/06
C12N 9/10

Abstract:

This disclosure generally relates to the use of microorganisms to make various functionalized polyketides through polyketoacyl-CoA thiolase-catalyzed non-decarboxylative condensation reactions instead of decarboxylative reactions catalyzed by polyketide synthases. Native or engineered polyketoacyl-CoA thiolases catalyze the non-decarboxylative Claisen condensation in an iterative manner (i.e. multiple rounds) between two either unsubstituted or functionalized ketoacyl-CoAs (and polyketoacyl-CoAs) serving as the primers and acyl-CoAs serving as the extender unit to generate (and elongate) polyketoacyl-CoAs. Before the next round of polyketoacyl-CoA thiolase reaction, the β-keto group of the polyketide chain of polyketoacyl-CoA can be reduced and modified step-wise by 3-OH-polyketoacyl-CoA dehydrogenase or polyketoenoyl-CoA hydratase or polyketoenoyl-CoA reductase. Dehydrogenase converts the β-keto group to β-hydroxy group. Hydratase converts the β-hydroxy group to α-β-double-bond. Reductase converts the α-β-double-bond to single bond. Spontaneous or thioesterase catalyzed termination reaction terminates the elongation of polyketide chain of polyketoacyl-CoA at any point through CoA removal and spontaneous reactions rearrange the structure, generating the final functional polyketide products.

US Patent:

20210002677, Jan 7, 2021

Filed:

Sep 15, 2020

Appl. No.:

17/021338

Inventors:

Ramon GONZALEZ - Tampa FL, US
Seokjung CHEONG - Emeryville CA, US
James M. CLOMBURG - Tampa FL, US

International Classification:

C12P 7/42
C12P 5/02
C12P 7/16
C12N 1/20

Abstract:

The use of microorganisms to make omega- and/or omega-1-functionalized products through an iterative carbon chain elongation pathway that we call a reverse beta oxidation pathway. The pathway uses omega-functionalized CoA thioesters as primers and acetyl-CoA as the extender unit in a non-decarboxylative Claisen condensation, and then uses beta oxidation or fatty acid synthesis enzymes to complete the cycle, via reductase, dehydratase and reductase reactions. Various termination enzymes that act on the functionalized beta-keto acyl-CoA intermediates of the pathway and produce omega or omega-1 functionalized products. The action of termination enzymes on such intermediates yield a large variety of products.

US Patent:

20200325502, Oct 15, 2020

Filed:

Mar 13, 2020

Appl. No.:

16/818642

Inventors:

Ramon GONZALEZ - Tampa FL, US
James M. CLOMBURG - Houston TX, US
Seokjung CHEONG - Emeryville CA, US

International Classification:

C12P 7/42
C12N 9/00
C12N 9/10
C12N 9/04
C12N 9/88
C12N 9/02
C12N 9/16

Abstract:

The use of microorganisms to make alpha-functionalized chemicals and fuels, (e.g. alpha-functionalized carboxylic acids, alcohols, hydrocarbons, amines, and their beta-, and omega-functionalized derivatives), by utilizing an iterative carbon chain elongation pathway that uses functionalized extender units. The core enzymes in the pathway include thiolase, dehydrogenase, dehydratase and reductase. Native or engineered thiolases catalyze the condensation of either unsubstituted or functionalized acyl-CoA primers with an alpha-functionalized acetyl-CoA as the extender unit to generate alpha-functionalized β-keto acyl-CoA. Dehydrogenase converts alpha-functionalized β-keto acyl-CoA to alpha-functionalized β-hydroxy acyl-CoA. Dehydratase converts alpha-functionalized β-hydroxy acyl-CoA to alpha-functionalized enoyl-CoA. Reductase converts alpha-functionalized enoyl-CoA to alpha-functionalized acyl-CoA. The platform can be operated in an iterative manner (i.e. multiple turns) by using the resulting alpha-functionalized acyl-CoA as primer and the aforementioned alpha-functionalized extender unit in subsequent turns of the cycle. Termination pathways acting on any of the four alpha-functionalized CoA thioester intermediates terminate the platform and generate various alpha-functionalized carboxylic acids, alcohols and amines with different β-reduction degree.

US Patent:

20200277638, Sep 3, 2020

Filed:

Mar 11, 2020

Appl. No.:

16/815790

Inventors:

Ramon GONZALEZ - Tampa FL, US
James M. CLOMBURG - Tampa FL, US

International Classification:

C12P 7/64

Abstract:

Methods of using microorganisms to make chemicals and fuels, including carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives are described. Native or engineered thiolases are used condense a growing acyl-ACP and acetyl-ACP in combination with type II fatty acid synthesis. The resulting fatty acid biosynthesis cycle has an ATP yield analogous to the functional reverse β-oxidation cycle.