Randy M Mccormick

US Patent:

6613211, Sep 2, 2003

Filed:

Aug 17, 2000

Appl. No.:

09/642607

Inventors:

Randy M. McCormick - Santa Clara CA
Gary J. Ciambrone - Redwood City CA
Ian Gibbons - Portola Valley CA

Assignee:

Aclara BioSciences, Inc. - Mountain View CA

International Classification:

C02F 140

US Classification:

204601

Abstract:

Cell based assays are performed in a microfluidic device, where the cells are introduced into a reservoir and are contacted with one or more agents prior to or during their residence in the reservoir or in a capillary channel connected to the reservoir. The cells are moved by electrokinesis individually from the reservoir to a detector, where the status of the cells as a result of contacting said agents is determined. Conditions are provided for moving the cells electrophoretically or by electroosmotic force, where the cells may be viable or fixed, natural or genetically modified.

US Patent:

20020112959, Aug 22, 2002

Filed:

Oct 4, 2001

Appl. No.:

09/972709

Inventors:

Qifeng Xue - Fremont CA, US
Ian Gibbons - Portola Valley CA, US
Randy McCormick - Santa Clara CA, US

International Classification:

G01N027/26
C12Q001/68
G01N027/447

US Classification:

204/453000, 435/006000, 204/604000

Abstract:

Methods, devices, and systems for unbiased transport of materials on a microfluidic device are disclosed, including methods of maintaining the starting composition of an analyte during transport, and methods of simultaneously analyzing both cationic and anionic components of an analyte. Analyte is loaded into a four-way junction of channels by controlled differential pressure applied to the channels. After analyte loading, an electrical potential is established, forcing charged species into at least one of two separation channels.

US Patent:

60568607, May 2, 2000

Filed:

Sep 18, 1997

Appl. No.:

8/933162

Inventors:

M. Goretty Alonso Amigo - Santa Clara CA
Randy M. McCormick - Santa Clara CA

Assignee:

ACLARA Biosciences, Inc. - Mountain View CA

International Classification:

G01N 2726
G01N 27447

US Classification:

204454

Abstract:

Electrophoretic chambers having at least a region of surface modification, and methods for their fabrication, are provided. In some embodiments the subject chambers include in the region of surface modification an anchoring polymeric layer interpenetrating the surface of the chamber and an electrophoretic polymeric layer copolymerized with the anchoring polymeric layer. The subject chambers are prepared by sequentially contacting the chamber surface with a first monomer capable of interpenetrating the surface and a second monomer capable of copolymerization with the first monomer, followed by copolymerization of the first and second monomers. In other embodiments an electrophoretic polymeric layer is noncovalently bound on the surface of a rigid polymereic base material without the aid of a separate anchoring polymeric layer. The subject devices find use in any of a variety of electrophoretic applications in which entities are moved through a medium under the influence of an applied electric field.

US Patent:

49239783, May 8, 1990

Filed:

Dec 28, 1987

Appl. No.:

7/138038

Inventors:

Randy M. McCormick - Wilmington DE

Assignee:

E. I. Du Pont de Nemours & Company - Wilmington DE

International Classification:

C07H 2100
C07K 318
C07K 320

US Classification:

536 27

Abstract:

The process for separating the proteinaceous materials from nucleic acids involves contacting a solution containing the proteinaceous materials and nucleic acids with a solid phase extraction material capable of binding proteins to form bound and unbound fractions and then isolating the unbound fraction containing the nucleic acids.

US Patent:

56162278, Apr 1, 1997

Filed:

Jun 26, 1995

Appl. No.:

8/494940

Inventors:

Randy M. McCormick - Santa Clara CA

Assignee:

Dionex Corporation - Sunnyvale CA

International Classification:

C25B 1500
G01N 2726

US Classification:

204457

Abstract:

A method for extending the useful lifetime and improving the reproducibility of electrophoresis gels, particularly gel-filled capillaries. The polarity of the applied electric field imposed across the gel is periodically reversed between one or more electrophoretic runs.

US Patent:

59001305, May 4, 1999

Filed:

Jun 18, 1997

Appl. No.:

8/878447

Inventors:

Dominic Benvegnu - La Honda CA
Randy M. McCormick - Santa Clara CA

Assignee:

Alcara BioSciences, Inc. - Hayward CA

International Classification:

G01N 2726

US Classification:

204453

Abstract:

A method and device are provided for transporting a liquid sample into a third microchannel from an intersection of at least a first, a second, and a fourth microchannel, by stages. In a first stage, liquid sample is moved in and from the fourth microchannel through the intersection and into the second microchannel and concurrently carrier liquid is moved in and from the first and third microchannels through the intersection and into the second microchannel. Thereafter in a second stage, at least part of the contents of the intersection is moved into the third channel and concurrently a part of the contents of the second and fourth microchannels is moved through the intersection and into the third microchannel. Thereafter in a third stage, carrier liquid is moved from the first microchannel simultaneously through the intersection and into the second, third, and fourth microchannels. In some embodiments the liquid sample and the carrier liquid are moved electrokinetically, that is, by application of an electric field to segments of the microchannels.

US Patent:

54416132, Aug 15, 1995

Filed:

Dec 3, 1993

Appl. No.:

8/161942

Inventors:

Randy M. McCormick - Santa Clara CA
Roy D. Rocklin - Sunnyvale CA

Assignee:

Dionex Corporation - Sunnyvale CA

International Classification:

G01N 2726
G01N 27447

US Classification:

2041801

Abstract:

In capillary electrophoresis systems, real-time monitoring and measurement of the electroosmotic flow through a separation capillary is accomplished by coupling the outlet of the separation capillary to an electrically-conductive junction. In one embodiment, this junction is an ion-impermeable or an ion-exchange membrane unit that preferentially exchanges ions having a charge opposite to analyte ions of interest. Within a downstream region of the junction, all axial incremental voltage from the electroosmotic voltage source is terminated, which ensures that downstream electrolyte ion movement is passive, due to active flow created upstream when an incremental axial voltage existed. Upstream electrolyte ion flux is proportional to C. sub. 1. multidot. (. mu. sub. e +. mu. sub. eo), where C. sub. 1 is the upstream concentration of the electrolyte ion of interest,. mu. sub.

US Patent:

55608116, Oct 1, 1996

Filed:

Mar 21, 1995

Appl. No.:

8/408683

Inventors:

Jonathan Briggs - Los Altos Hills CA
Randy M. McCormick - Santa Clara CA
David W. Hoyt - Saratoga CA

Assignee:

Seurat Analytical Systems Incorporated - Sunnyvale CA

International Classification:

B01D 5702
B01D 5942
C07K 126
C25B 700

US Classification:

204451

Abstract:

This invention involves method and apparatus for multiplexing electrophoresis analysis. An array of samples in multi well plates are simultaneously transferred to an array of electrophoresis column where electrophoresis is simultaneously carried out followed by analysis of the columns. The methods and apparatus of this invention are, for example, useful for DNA analysis, including sequencing, and for measuring reactions between specifically binding proteins and their binding partners.