Ravinder K Gupta

US Patent:

6368864, Apr 9, 2002

Filed:

May 5, 2000

Appl. No.:

09/566298

Inventors:

Chiranjit Deka - Miami FL
Song Y. Lee - Davie FL
Gene G.-Y. Shen - Diamond Bar CA
Stephen Szydlo - Miami FL
Ravinder Gupta - Pembroke Pines FL

Assignee:

Coulter International Corp. - Miami FL

International Classification:

G01N 3100

US Classification:

436 10, 436 8, 436 63, 436166, 436172, 436800, 2524081, 435 2, 435 29, 435 34

Abstract:

A dye compound having the formula I: wherein, n is 0, 1, 2, or 3; R1 is H, alkyl, or an alkoxy group; R2 is CH (CH ) OH, wherein m is 0, 1, 2, or 3; X is O, S, or C(CH ) ; R is CH , CH(CH ) , CH CH OH, alkyl, alkylsulfonate, or hydroxyalkyl and B- is a counteranion. Red- and blue-excitable dyes based on the above chemical structure are described, and reagents containing them are described for use in staining nucleic acid, more particularly for staining reticulocytes. Also described are methods for detecting reticulocytes using such compositions.

US Patent:

6551831, Apr 22, 2003

Filed:

May 4, 2001

Appl. No.:

09/849155

Inventors:

Ravinder Gupta - Pembroke Pines FL
Song Y. Lee - Davie FL
Gene G.-Y. Shen - Diamond Bar CA
Stephen Szydlo - Miami FL
Chiranjit Deka - Miami MA

Assignee:

Coulter International Corp. - Miami FL

International Classification:

G01N 3100

US Classification:

436 10, 436 8, 436 63, 436166, 436172, 436800, 2524081, 435 2, 435 29, 435 34

Abstract:

The dyes of the present invention are useful for many purposes that include markers or tags for detecting the presence of a molecule or compound to which they are bound. The dyes may be either red-excitable or blue-excitable. The dyes of the invention are particularly well suited for staining of nucleic acids. For example, these dyes are particularly suitable for staining of RNA in reticulocytes. In another exemplary application, these dyes are suitable for staining DNA in nucleated red blood cells. Typically, when used in staining of nucleic acids, the dyes are formulated into reagent solutions. In addition, the invention provides compositions and methods for facilitating rapid transport of dye molecules through a cell membrane. Such rapid staining requires that a sample be contacted with a dye composition of the invention in the presence of at least one surfactant and optionally, a sulfonic acid or a salt thereof.

US Patent:

6692968, Feb 17, 2004

Filed:

Sep 20, 2001

Appl. No.:

09/957234

Inventors:

Alexander Burshteyn - Hialeah FL
John W. Joubran - Escondido CA
Nazle Kuylen - Miami FL
Frank J. Lucas - Boca Raton FL
Carlos L. Aparicio - Miami FL
Michael L. Bell - Fullerton CA
Ravinder Gupta - Pembroke Pines FL
Maria Elena Insausti - Miami FL
Jack D. McNeal - Long Beach CA
Paul W. Price - Miami FL
Sandra Socarras - Miami FL

Assignee:

Coulter International Corp. - Miami FL

International Classification:

G01N 134

US Classification:

436 63, 210767, 422101, 4353081, 436178

Abstract:

A method for utilizing a filtration device for removing interferants from a test sample containing a mixture of a composition of interest and interferants in an automated apparatus is disclosed. The filtration device includes a microporous hollow fiber membrane having a plurality of pores sized to retain the composition of interest while allowing smaller diameter interferants to pass through the membrane.

US Patent:

8367842, Feb 5, 2013

Filed:

Jul 15, 2010

Appl. No.:

12/836747

Inventors:

Hashem Akhavan-Tafti - Howell MI, US
Renuka De Silva - Northville MI, US
Guoping Wang - Novi MI, US
Robert A. Eickholt - Troy MI, US
Ravinder K. Gupta - Pembroke Pines FL, US
Lakshmi S. Kaanumalle - Schenectady NY, US

Assignee:

Beckman Coulter, Inc. - Brea CA

International Classification:

C07D 277/62

US Classification:

548178

Abstract:

The present invention provides fluorescent dyes that are based on firefly luciferin structure. These dyes are optimally excited at shorter wavelengths and have Stokes shift of at least 50 nm. The fluorescent dyes of the invention are useful for preparation of dye-conjugates, which can be used in detection of an analyte in a sample.

US Patent:

20040132208, Jul 8, 2004

Filed:

Dec 19, 2003

Appl. No.:

10/741395

Inventors:

Alexander Burshteyn - Pembroke Pines FL, US
John Joubran - Escondido CA, US
Nazle Kuylen - Miami FL, US
Frank Lucas - Boca Raton FL, US
Carlos Aparicio - Miami FL, US
Michael Bell - Fullerton CA, US
Ravinder Gupta - Pembroke Pines FL, US
Maria Insausti - Miami FL, US
Jack McNeal - Long Beach CA, US
Paul Price - Miami FL, US
Sandra Socarras - Miami FL, US

Assignee:

Coulter International Corp. - Miami FL

International Classification:

G01N001/18

US Classification:

436/178000

Abstract:

A method for utilizing a filtration device for removing interferants from a test sample containing a mixture of a composition of interest and interferants in an automated apparatus is disclosed. The filtration device includes a microporous hollow fiber membrane having a plurality of pores sized to retain the composition of interest while allowing smaller diameter interferants to pass through the membrane.

US Patent:

20100151493, Jun 17, 2010

Filed:

Dec 10, 2009

Appl. No.:

12/635063

Inventors:

Joseph Wolfers - La Bouilladisse, FR
Fabrice Malergue - Marseille, FR
Ravinder K. Gupta - Pembroke Pines FL, US
Lori A. Charie - Pembroke Pines FL, US
Meryl Forman - Miami FL, US
Emmanuel Gautherot - Marseille, FR

Assignee:

Beckman Coulter, Inc. - Brea CA

International Classification:

G01N 33/53
G01N 33/543

US Classification:

435 72, 436518

Abstract:

Novel compositions containing (i) ligand conjugated phycobiliprotein tandem dyes and (ii) unconjugated phycobiliproteins are provided wherein the phycobiliproteins are derived from the same bacterial or eukaryotic algae species. The phycobiliproteins must be the same or different, but must contain some non-crosslinked subunits which exchange. Also provided are methods for preparing these compositions, kits containing these compositions or components of the same, and methods of using these compositions for cellular and non-cellular analysis.

US Patent:

20130131324, May 23, 2013

Filed:

Jan 24, 2013

Appl. No.:

13/748756

Inventors:

BECKMAN COULTER, INC. - Brea CA, US
Renuka De Silva - Northville MI, US
Guoping Wang - Novi MI, US
Robert A. Eickholt - Troy MI, US
Ravinder Gupta - Pembroke Pines FL, US
Lakshmi S. Kaanumalle - Schenectady NY, US

Assignee:

BECKMAN COULTER, INC. - Brea CA

International Classification:

C07D 417/04

US Classification:

5303913

Abstract:

The present invention provides fluorescent dyes that are based on firefly luciferin structure. These dyes are optimally excited at shorter wavelengths and have Stokes shift of at least 50 nm. The fluorescent dyes of the invention are useful for preparation of dye-conjugates, which can be used in detection of an analyte in a sample.

US Patent:

62710354, Aug 7, 2001

Filed:

Oct 20, 1998

Appl. No.:

9/175495

Inventors:

Chiranjit Deka - Miami FL
Kristie M. Gordon - Coral Gables FL
Ravinder Gupta - Pembroke Pines FL
Allan Horton - Miami FL

Assignee:

Coulter International Corp. - Miami FL

International Classification:

G01N 3100

US Classification:

436 10

Abstract:

A rapid fluorescence staining method for facilitating flow cytometry analysis of reticulocytes is described. The method comprises contacting cells with a cocktail containing a detergent, sphering agent, and a cell impermeable dye, such as TO-PRO-3, for about one minute. Advantageously, the inventors have found that the cocktail permits the dye to penetrate the cell membrane rapidly.