Raymond M Lowe

US Patent:

8110360, Feb 7, 2012

Filed:

Jan 8, 2010

Appl. No.:

12/684874

Inventors:

Zach Serber - Emeryville CA, US
Raymond Lowe - Emeryville CA, US
Jeffrey A. Ubersax - Emeryville CA, US
Sunil S. Chandran - Emeryville CA, US

Assignee:

Amyris, Inc. - Emeryville CA

International Classification:

C12Q 1/68

US Classification:

435 612, 435 912, 435483, 536 231

Abstract:

The present invention provides compositions and methods for rapid assembly of one or more assembled polynucleotides from a plurality of component polynucleotides. The methods of the invention utilize circular nucleic acid vectors that comprise a DNA segment D flanked by an annealable linker sequence, annealable linker sequence pairs LA and LB, or annealable linker sequence/primer binding segment pairs LA and PB or PA and LB. Restriction endonuclease digestion of a plurality of vectors containing the DNA segments to be assembled generates a plurality of DNA fragments comprising the elements PA-D-LB, LA-D-LB, and LA-D-PB or D-LB, LA-D-LB, and LA-D. The sequences of annealable linker sequences LA and LB provide complementary termini to the DNA fragments, which are utilized in host cell mediated homologous recombination or together with primer binding segments PA and PB in a polymerase cycling assembly reaction for the ordered assembly of the various DNA segments into one or more assembled polynucleotides.

US Patent:

8221982, Jul 17, 2012

Filed:

Nov 19, 2009

Appl. No.:

12/622401

Inventors:

Zach Serber - Emeryville CA, US
Raymond Lowe - Emeryville CA, US
Jeffrey A. Ubersax - Emeryville CA, US
Sunil S. Chandran - Emeryville CA, US

Assignee:

Amyris, Inc. - Emeryville CA

International Classification:

C12Q 1/68

US Classification:

435 612, 435 912, 435483, 536 231

Abstract:

The present invention provides compositions and methods for rapid assembly of one or more assembled polynucleotides from a plurality of component polynucleotides. The methods of the invention utilize circular nucleic acid vectors that comprise a DNA segment D flanked by an annealable linker sequence, annealable linker sequence pairs LA and LB, or annealable linker sequence/primer binding segment pairs LA and PB or PA and LB. Restriction endonuclease digestion of a plurality of vectors containing the DNA segments to be assembled generates a plurality of DNA fragments comprising the elements PA-D-LB, LA-D-LB, and LA-D-PB or D-LB, LA-D-LB, and LA-D. The sequences of annealable linker sequences LA and LB provide complementary termini to the DNA fragments, which are utilized in host cell mediated homologous recombination or together with promer binding segments PA and PB in a polymerase cycling assembly reaction for the ordered assembly of the various DNA segments into one or more assembled polynucleotides.

US Patent:

8546136, Oct 1, 2013

Filed:

Mar 26, 2012

Appl. No.:

13/430322

Inventors:

Zach Serber - Emeryville CA, US
Raymond Lowe - Emeryville CA, US
Jeffrey A. Ubersax - Emeryville CA, US
Sunil S. Chandran - Emeryville CA, US

Assignee:

Amyris, Inc. - Emeryville CA

International Classification:

C12N 15/00

US Classification:

4353201, 43525233, 536 231

Abstract:

The present invention provides compositions and methods for rapid assembly of one or more assembled polynucleotides from a plurality of component polynucleotides. The methods of the invention utilize circular nucleic acid vectors that comprise a DNA segment D flanked by an annealable linker sequence, annealable linker sequence pairs LA and LB, or annealable linker sequence/primer binding segment pairs LA and PB or PA and LB. Restriction endonuclease digestion of a plurality of vectors containing the DNA segments to be assembled generates a plurality of DNA fragments comprising the elements PA-D-LB, LA-D-LB, and LA-D-PB or D-LB, LA-D-LB, and LA-D. The sequences of annealable linker sequences LA and LB provide complementary termini to the DNA fragments, which are utilized in host cell mediated homologous recombination or together with promer binding segments PA and PB in a polymerase cycling assembly reaction for the ordered assembly of the various DNA segments into one or more assembled polynucleotides.

US Patent:

20050130974, Jun 16, 2005

Filed:

Oct 18, 2004

Appl. No.:

10/967943

Inventors:

Usha Ramesh - Cupertino CA, US
Rajinder Singh - Belmont CA, US
Donald Payan - Hillsborough CA, US
Francesco Parlati - San Francisco CA, US
Raymond Lowe - San Francisco CA, US
Gary Look - Santa Clara CA, US

International Classification:

A61K031/519
A61K031/428
A61K031/4745

US Classification:

514248000, 514249000, 514260100, 514301000, 514367000

Abstract:

This invention describes compounds and pharmaceutical compositions useful as ubiquitin agent inhibitors. The compounds and pharmaceutical compositions of the invention are useful as inhibitors of the biochemical pathways of organisms in which ubiquitination is involved. The invention also comprises the use of the compounds and pharmaceutical compositions of the invention for the treatment of conditions that require inhibition of ubiquitination. Furthermore, the invention comprises methods of inhibiting ubiquitination in a cell comprising contacting a cell in which inhibition of ubiquitination is desired with a pharmaceutical composition according to the invention.