Rebecca Bonner

US Patent:

6372450, Apr 16, 2002

Filed:

Nov 13, 2000

Appl. No.:

09/711495

Inventors:

Robert E. Hurst - Oklahoma City OK
Rebecca B. Bonner - Oklahoma City OK

Assignee:

Southpac Trust Intl. Inc.

International Classification:

C12Q 106

US Classification:

435 405, 435 4051, 435 721, 435975, 435 72, 435 4052, 435412

Abstract:

A composition and treatment method for fixating cells and for preparing them for examination. In particular, the invention contemplates compositions effective in inhibiting crystal formation in the cell sample and effective in enhancing cell adherence to examination slides.

US Patent:

55993311, Feb 4, 1997

Filed:

Jan 9, 1995

Appl. No.:

8/370149

Inventors:

George P. Hemstreet - Oklahoma City OK
Karl H. Bergey - Norman OK
Robert E. Hurst - Oklahoma City OK
Rebecca B. Bonner - Oklahoma City OK

Assignee:

The Board of Regents of the University of Oklahoma - Norman OK

International Classification:

A61B 500

US Classification:

604317

Abstract:

A fluid collection device having a primary container and a secondary container and a two stage fixation system for facilitating and controlling the fixation of cells in a sample of a biological fluid such as urine. The primary container generally comprises a plastic cup having a filtering apparatus disposed within it for filtering cells from the fluid. The primary container may contain a first stage fixative. The device further comprises a cover comprising a pressurizing chamber for forcing fluid through the filter and fixative delivery chamber for dispensing a fixative into the primary container. The filtered fluid may be reserved within a secondary container attached to the primary container. The filter having the cells retained on its surface is removable from the primary container for the purpose of laboratory analyses of the cells.

US Patent:

58244956, Oct 20, 1998

Filed:

Mar 20, 1997

Appl. No.:

8/821378

Inventors:

George P. Hemstreet - Oklahoma City OK
Robert E. Hurst - Oklahoma City OK
Rebecca B. Bonner - Oklahoma City OK

Assignee:

Southpac Trust International, Inc.

International Classification:

C12Q 106
G01N 3353

US Classification:

435 405

Abstract:

A composition and treatment method for fixation cells and for preparing them for examination. In particular, the invention contemplates compositions effective in inhibiting crystal formation in the cell sample and effective in enhancing cell adherence to examination slides.

US Patent:

59975199, Dec 7, 1999

Filed:

Apr 6, 1998

Appl. No.:

9/055837

Inventors:

George P. Hemstreet - Oklahoma City OK
Karl H. Bergey - Norman OK
Robert E. Hurst - Oklahoma City OK
Rebecca B. Bonner - Oklahoma City OK

Assignee:

The Board of Regents of The University of Oklahoma - Norman OK

International Classification:

A61B 500
A61M 100

US Classification:

604317

Abstract:

A fluid collection device having a primary container and a secondary container and a two stage fixation system for facilitating and controlling the fixation of cells in a sample of a biological fluid such as urine. The primary container generally comprises a plastic cup having a filtering apparatus disposed within it for filtering cells from the fluid. The primary container may contain a first stage fixative. The device further comprises a cover comprising a pressurizing chamber for forcing fluid through the filter and fixative delivery chamber for dispensing a fixative into the primary container. The filtered fluid may be reserved within a secondary container attached to the primary container. The filter having the cells retained on its surface is removable from the primary container for the purpose of laboratory analyses of the cells.

US Patent:

57337217, Mar 31, 1998

Filed:

Dec 20, 1992

Appl. No.:

7/984191

Inventors:

George P. Hemstreet - Oklahoma City OK
Robert E. Hurst - Oklahoma City OK
Rebecca B. Bonner - Oklahoma City OK
Jian Yu Rao - Edmond OK

Assignee:

The Board of Regents of the University of Oklahoma - Norman OK

International Classification:

G01N 33574
G01N 3353

US Classification:

435 6

Abstract:

A system for evaluating one or more biochemical markers for evaluating individual cancer risk, cancer diagnosis and for monitoring therapeutic effectiveness and cancer recurrence, particularly of bladder cancer. The system uses automated quantitative fluorescence image analysis of a cell sample collected from a body organ. Cells are treated with a fixative solution which inhibits crystal formation. Cell images are selected and stored as grey level images for further analysis. Cell images may be corrected for autofluorescence using a novel autofluorescence correction method. A neural net computer may be used to distinguish true-positive images from false-positive images to improve accuracy of cancer risk assessment. Cells having images positive for a marker amy be compared to threshold quantities related to predetermined cancer risk.

US Patent:

61941654, Feb 27, 2001

Filed:

Oct 2, 1998

Appl. No.:

9/165455

Inventors:

George P. Hemstreet - Oklahoma City OK
Robert E. Hurst - Oklahoma City OK
Rebecca B. Bonner - Oklahoma City OK

Assignee:

The Board of Regents of the University of Oklahoma - Norman OK

International Classification:

C12Q 106
G01N 3353

US Classification:

435 405

Abstract:

A composition and treatment method for fixating cells and for preparing them for examination. In particular, the invention contemplates compositions effective in inhibiting crystal formation in the cell sample and effective in enhancing cell adherence to examination slides.

US Patent:

53802896, Jan 10, 1995

Filed:

Jan 19, 1993

Appl. No.:

8/005488

Inventors:

George P. Hemstreet - Oklahoma City OK
Karl H. Bergey - Norman OK
Robert E. Hurst - Oklahoma City OK
Rebecca B. Bonner - Oklahoma City OK

Assignee:

The Board of Regents of the University of Oklahoma - Norman OK

International Classification:

A61M 100

US Classification:

604317

Abstract:

A fluid collection device having a primary container and a secondary container and a two stage fixation system for facilitating and controlling the fixation of cells in a sample of a biological fluid such as urine. The primary container generally comprises a plastic cup having a filtering apparatus disposed within it for filtering cells from the fluid. The primary container may contain a first stage fixative. The device further comprises a cover comprising a pressurizing chamber for forcing fluid through the filter and fixative delivery chamber for dispensing a fixative into the primary container. The filtered fluid may be reserved within a secondary container attached to the primary container. The filter having the cells retained on its surface is removable from the primary container for the purpose of laboratory analyses of the cells.

US Patent:

57416486, Apr 21, 1998

Filed:

Feb 9, 1996

Appl. No.:

8/605342

Inventors:

George P. Hemstreet - Oklahoma City OK
Robert E. Hurst - Oklahoma City OK
Rebecca B. Bonner - Oklahoma City OK
Jian Yu Rao - Edmond OK

Assignee:

The Board of Regents of the University of Oklahoma - Norman OK

International Classification:

G01N 33574
G01N 3353

US Classification:

435 6

Abstract:

A system for evaluating one or more biochemical markers for evaluating individual cancer risk, cancer diagnosis and for monitoring therapeutic effectiveness and cancer recurrence, particularly of bladder cancer. The system uses automated quantitative fluorescence image analysis of a cell sample collected from a body organ. Cells are treated with a fixative solution which inhibits crystal formation. Cell images are selected and stored as grey level images for further analysis. Cell images may be corrected for autofluorescence using a novel autofluorescence correction method. A neural net computer may be used to distinguish true-positive images from false-positive images to improve accuracy of cancer risk assessment. Cells having images positive for a marker amy be compared to threshold quantities related to predetermined cancer risk.