Reid D Frederick

Address:

46882 Blackberry Ct, Sterling, VA 20164

VIN:

JTEHP21AX70225436

Make:

Toyota

Model:

Highlander 4WD 4dr V6 (N

Year:

2007

US Patent:

7097975, Aug 29, 2006

Filed:

Jul 19, 2001

Appl. No.:

09/907818

Inventors:

Reid D. Frederick - Ashburn VA, US

Assignee:

The United States of America as represented by the Secretary of Agriculture - Washington DC

International Classification:

C07H 21/04
C12Q 1/68

US Classification:

435 6, 435 912, 536 231, 536 243, 536 2432

Abstract:

Soybean rust occurs in many countries throughout Asia, Australia, Africa, and South America. The causal agents of soybean rust are two closely related fungi, and , which are differentiated based upon morphological characteristics of the telia. Determination of the nucleotide sequence of the internal transcribed spacer (ITS) region revealed greater than 99%/95% nucleotide sequence similarity among isolates of either or , but only 80% sequence similarity between the two species. Utilizing differences within the ITS region, four sets of PCR primers were designed specifically for , and two sets of PCR primers were made specific to. Classical and real-time fluorescent PCR assays were developed to identify and differentiate between and.

US Patent:

63161958, Nov 13, 2001

Filed:

Dec 23, 1999

Appl. No.:

9/471016

Inventors:

Reid D. Frederick - Ashburn VA
Paul W. Tooley - Braddock Heights MD
Morris R. Bonde - Middletown MD
David A. Knorr - San Leandro CA
Gary L. Peterson - Frederick MD
Norman W. Schaad - Myersville MD

Assignee:

The United States of America as represented by the Secretary of Agriculture - Washington DC

International Classification:

C07H 2104
C12Q 168

US Classification:

435 6

Abstract:

Karnal bunt of wheat is caused by Tilletia indica Mitra. Recently, teliospores morphologically resembling T. indica were isolated from bunted ryegrass seeds and wheat seed washes. Previously developed PCR assays failed to differentiate T. indica from the newly discovered ryegrass pathogen, T. walkeri. The nucleotide sequence of a 2. 3-kb region of mitochondrial DNA, previously amplified by PCR only from T. indica, was determined for three isolates of T. indica and for three isolates of T. walkeri. There was greater than 99% identity within either the T. indica group or the T. walkeri group of isolates, whereas there was approximately 3% divergence between isolates of these two Tilletia species. Five sets of PCR primers were made specific to T. indica, and three sets were designed specifically for T.