Renee Denise Howell

US Patent:

20120178077, Jul 12, 2012

Filed:

Aug 31, 2011

Appl. No.:

13/223258

Inventors:

Fernando DeCastro - Boyds MD, US
Renee Howell - Rockville MD, US
Sami Kanderian - Germantown MD, US
Johnathan S. Coursey - Germantown MD, US
Kenton C. Hasson - Germantown MD, US
Scott Sundberg - Rockville MD, US

Assignee:

CANON U.S. LIFE SCIENCES, INC. - Rockville MD

International Classification:

G01N 25/04
C09K 3/00
G01K 15/00
C07H 21/04

US Classification:

435 3, 536 231, 2524081, 374 1, 374E15001

Abstract:

The present invention relates to the use of one or more amplicons as temperature calibrators. In some embodiments, the calibrators may be used to calibrate the temperature of a microfluidic channel in which amplification and/or melt analysis is performed. In some embodiments, the amplicons may be genomic, ultra conserved elements and/or synthetic. The amplicon(s) may have a known or expected melt temperature(s). The calibrators may be added to primers of study or may follow or lead the primers of study in the channel. The amplicon(s) may be amplified and melted, and the temperature(s) at which the amplicon(s) melted may be determined. The measured temperature(s) may be compared to the known temperature(s) at which the amplicon(s) was expected to melt. The difference(s) between the measured and expected temperatures may be used to calibrate/adjust one or more temperature control elements used to control and/or detect the temperature of the channel.

US Patent:

20130122493, May 16, 2013

Filed:

Nov 16, 2011

Appl. No.:

13/297970

Inventors:

LING XU - ROCKVILLE MD, US
RENEE HOWELL - ROCKVILLE MD, US

Assignee:

CANON U.S. LIFE SCIENCES, INC. - ROCKVILLE MD

International Classification:

C12Q 1/68
C12M 1/34
C07H 21/00

US Classification:

435 611, 435 612, 536 2433, 536 2431, 4352872

Abstract:

The present invention relates to methods, kits, probes, and systems for distinguishing between nucleotide variants that are close in proximity on a gene. The methods, kits, probes, and systems can include the use of a small amplicon assay in combination with two unlabeled probes in a high resolution thermal melting analysis of a biological sample containing a locus of interest in order to discern between disease-causing and benign variants that are close in proximity on a gene within the biological sample. The present invention also relates to method of detecting a disease in a patient based on the patient's genotype by determining whether the patient has a disease-causing variant at a locus of interest. The signature melt curves produced by the unlabeled probe tests can be analyzed using HRMA software to distinguish between disease-causing and benign variants that are close in proximity on a gene within the biological sample.

US Patent:

20120088236, Apr 12, 2012

Filed:

Jun 2, 2011

Appl. No.:

13/152260

Inventors:

Jacquline Yancy - Laurel MD, US
Lingxia Jiang - Boyds MD, US
Stephanie Buszczak - Rockville MD, US
Renee Howell - Rockville MD, US
Deborah Boles - Cary NC, US

Assignee:

CANON U.S. LIFE SCIENCES, INC. - Rockville MD

International Classification:

C12Q 1/68

US Classification:

435 611

Abstract:

The present invention relates to methods and systems for genome scanning using high resolution melting analysis for identifying mutations and/or variants in genes of interest.

US Patent:

20200264055, Aug 20, 2020

Filed:

Mar 16, 2020

Appl. No.:

16/820255

Inventors:

- Melville NY, US
Renee Howell - Rockville MD, US
Sami Kanderian - Germantown MD, US
Johnathan S. Coursey - Germantown MD, US
Kenton C. Hasson - Germantown MD, US
Scott Sundberg - Rockville MD, US

Assignee:

Canon U.S.A., Inc. - Melville NY

International Classification:

G01K 11/06
G01K 15/00
B01L 7/00
B01L 3/00
C12Q 1/6806

Abstract:

The present invention relates to the use of one or more amplicons as temperature calibrators. In some embodiments, the calibrators may be used to calibrate the temperature of a microfluidic channel in which amplification and/or melt analysis is performed. In some embodiments, the amplicons may be genomic, ultra conserved elements and/or synthetic. The amplicon(s) may have a known or expected melt temperature(s). The calibrators may be added to primers of study or may follow or lead the primers of study in the channel. The amplicon(s) may be amplified and melted, and the temperature(s) at which the amplicon(s) melted may be determined. The measured temperature(s) may be compared to the known temperature(s) at which the amplicon(s) was expected to melt. The difference(s) between the measured and expected temperatures may be used to calibrate/adjust one or more temperature control elements used to control and/or detect the temperature of the channel.

US Patent:

20170009279, Jan 12, 2017

Filed:

Sep 19, 2016

Appl. No.:

15/269362

Inventors:

- Rockville MD, US
Renee Howell - Rockville MD, US

Assignee:

Canon U.S. Life Sciences, Inc. - Rockville MD

International Classification:

C12Q 1/68

Abstract:

The present invention relates to methods, kits, probes, and systems for distinguishing between nucleotide variants that are close in proximity on a gene. The methods, kits, probes, and systems can include the use of a small amplicon assay in combination with two unlabeled probes in a high resolution thermal melting analysis of a biological sample containing a locus of interest in order to discern between disease-causing and benign variants that are close in proximity on a gene within the biological sample. The present invention also relates to method of detecting a disease in a patient based on the patient's genotype by determining whether the patient has a disease-causing variant at a locus of interest. The signature melt curves produced by the unlabeled probe tests can be analyzed using HRMA software to distinguish between disease-causing and benign variants that are close in proximity on a gene within the biological sample.