Richard G Allison

US Patent:

7256325, Aug 14, 2007

Filed:

Dec 8, 1998

Appl. No.:

09/206852

Inventors:

Richard F. Allison - Leslie MI, US
Jeanne Ohrnberger - Whitemore Lake MI, US
Rafaz Hoque - New York NY, US
Mark Desrosiers - Lexington KY, US

Assignee:

Board of Trustees operating Michigan State University - East Lansing MI

International Classification:

C12N 15/82
C12N 15/84
C12N 15/87
A01H 1/00
C12N 15/29
C12N 15/53

US Classification:

800292, 800260, 800278, 800294, 800312, 800313, 435189, 536 232, 536 236

Abstract:

Methods for the transformation of plants are provided. The methods of the present invention comprise the steps of a) contacting the meristematic tissue of a plant and an area of the plant below the contacted meristematic tissue to a power source, wherein the area below the meristematic tissue is contacted to a positive lead and the meristematic tissue is contacted with a DNA-containing medium which in turn is contacted with a negative lead, and b) applying low amperage current, thus causing the DNA to migrate from the DNA-containing medium into the cells of the meristematic tissue. The transformed plants may be grown to maturity. The mature plants may then self-pollinate and the seed from the plant harvested. Both the parent plant, seed from the parent plant, progeny plants and progeny seeds contain the introduced DNA.

US Patent:

7344888, Mar 18, 2008

Filed:

Mar 4, 2004

Appl. No.:

10/793454

Inventors:

Richard F Allison - Leslie MI, US
Jerri Gillett - East Lansing MI, US
Christy Mecey - Leslie MI, US

Assignee:

Board of Trustees operating Michigan State University - East Lansing MI

International Classification:

C07H 21/04
C12N 15/82
C12N 5/04

US Classification:

435468, 4353201, 435419, 536 241, 800278

Abstract:

A nucleic acid sequence of the blueberry red ringspot virus is disclosed. Also disclosed are putative promoter regions of the sequence and promoter regions capable of directing transgene expression in plants, including tissue-specific expression. Also disclosed are expression vectors, transformed plant cells and plants containing a blueberry red ringspot virus promoter and an encoded product for expression. Methods for diagnosis of blueberry red ringspot virus infection are also provided.

US Patent:

7696406, Apr 13, 2010

Filed:

Jul 2, 2004

Appl. No.:

10/561720

Inventors:

Richard F. Allison - Leslie MI, US

Assignee:

Board of Trustees Operating Michigan State University - East Lansing MI

International Classification:

C12N 15/82
C12N 15/87
A01H 1/00
C07H 21/04

US Classification:

800278, 800288, 536 241, 536 2372

Abstract:

A system for expression of a heterologous polypeptide in a transgenic host cell is disclosed. The system is based upon a transgene comprising a eukaryotic promoter operably linked to a DNA sequence comprising, in the 5′ to 3′ direction, a DNA sequence complementary to a sequence encoding a heterologous polypeptide, a DNA sequence complementary to an internal ribosome entry site, and a DNA sequence corresponding to a 3′ untranslated region of a positive strand single-stranded RNA virus. Following introduction of a stimulus, the host cell synthesizes an RNA molecule complementary to a recombinant RNA encoded by the transgene. The stimulus can be a positive strand single-stranded RNA virus or a nucleic acid thereof. Because the complement of the recombinant RNA comprises an internal ribosome entry site and a sequence encoding a heterologous polypeptide, the host cell can synthesize the heterologous polypeptide.

US Patent:

20090023909, Jan 22, 2009

Filed:

Feb 18, 2008

Appl. No.:

12/032927

Inventors:

Richard F. Allison - Leslie MI, US
Jerri Gillett - East Lansing MI, US
Christy Mecey - Leslie MI, US

Assignee:

Board of Trustees of Michigan State of University - East Lansing MI

International Classification:

C07H 21/04
A01H 5/00
C12Q 1/68

US Classification:

536 2432, 536 241, 800301, 435468, 435 6

Abstract:

A nucleic acid sequence of the blueberry red ringspot virus is disclosed. Also disclosed are putative promoter regions of the sequence and promoter regions capable of directing transgene expression in plants, including tissue-specific expression. Also disclosed are expression vectors, transformed plant cells and plants containing a blueberry red ringspot virus promoter and an encoded product for expression. Methods for diagnosis of blueberry red ringspot virus infection are also provided.