Richard W Gantt

US Patent:

8637287, Jan 28, 2014

Filed:

Jun 13, 2011

Appl. No.:

13/159097

Inventors:

Jon S. Thorson - Middleton WI, US
Richard W. Gantt - Roswell GA, US

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

C12N 9/10
C12N 1/00

US Classification:

435193, 435183

Abstract:

The present invention generally relates to materials and methods for exploiting glycosyltransferase reversibility for nucleotide diphosphate (NDP) sugar synthesis. The present invention provides engineered glycosyltransferase enzymes characterized by improved reaction reversibility and expanded sugar donor specificity as compared to corresponding non-mutated glycosyltransferase enzymes. Such reagents provide advantageous routes to NDP sugars for subsequent use in a variety of biomedical applications, including enzymatic and chemoenzymatic glycorandomization.

US Patent:

20090181854, Jul 16, 2009

Filed:

Jul 24, 2008

Appl. No.:

12/179531

Inventors:

Jon S. Thorson - Middleton WI, US
Gavin J. Williams - Madison WI, US
Richard W. Gantt - Roswell GA, US

Assignee:

WISCONSIN ALUMNI RESEARCH FOUNDATION - Madison WI

International Classification:

C40B 30/04
C12N 9/10

US Classification:

506 9, 435193

Abstract:

The present invention provides engineered glycosyltransferase enzymes and method for making and using the same possessing an expanded substrate specificity as compared to corresponding non-mutated glycosyltransferase enzymes. Such enzymes expand the variety of substrates that can be used in enzymatic glycosylation methods, including enzymatic glycorandomization, thereby providing increased diversity in chemical products.

US Patent:

20200102611, Apr 2, 2020

Filed:

Oct 1, 2019

Appl. No.:

16/590301

Inventors:

- San Francisco CA, US
Siyuan CHEN - San Mateo CA, US
Richard GANTT - San Francisco CA, US
Kristin D. BUTCHER - San Francisco CA, US
E. Hutson CHILTON - San Francisco CA, US

International Classification:

C12Q 1/6869
C40B 70/00
G16B 40/10
C40B 30/10

Abstract:

Provided herein are compositions, methods and systems relating to libraries of polynucleotides such that the libraries allow for accurate and efficient hybridization after binding to target sequences. Further provided herein are probes, blockers, additives, buffers, and methods that result in improved hybridization. Such compositions and methods are useful for improvement of Next Generation Sequencing applications, such as reducing off-target binding or reducing workflow times.

US Patent:

20200017907, Jan 16, 2020

Filed:

May 20, 2019

Appl. No.:

16/417023

Inventors:

- San Francisco CA, US
Siyuan CHEN - San Mateo CA, US
Richard GANTT - San Francisco CA, US
Kristin D. BUTCHER - San Francisco CA, US
E. Hutson CHILTON - San Francisco CA, US

International Classification:

C12Q 1/6869
G16B 40/10
C40B 30/10
C40B 70/00

Abstract:

Provided herein are compositions, methods and systems relating to libraries of polynucleotides such that the libraries allow for accurate and efficient hybridization after binding to target sequences. Further provided herein are probes, blockers, additives, buffers, and methods that result in improved hybridization. Such compositions and methods are useful for improvement of Next Generation Sequencing applications, such as reducing off-target binding or reducing workflow times.