Richard Dale Gibbs

US Patent:

6340750, Jan 22, 2002

Filed:

Dec 14, 1999

Appl. No.:

09/460718

Inventors:

Kevin Burgess - Bryan TX
Richard Gibbs - Houston TX

Assignee:

The Texas AM University System - College Station TX

International Classification:

C07H 1904

US Classification:

536 266, 536 2532, 25230116, 422 8206, 422 8207

Abstract:

Fluorescent energy transfer cassettes that allow through bond energy transfer and have a succinimidyl ester functionality suitable for affecting them to biomolecules or provided and are applied to high throughput DNA sequencing.

US Patent:

6975943, Dec 13, 2005

Filed:

Sep 24, 2001

Appl. No.:

09/969111

Inventors:

Richard Gibbs - Houston TX, US
Allan Bradley - Cambridge, GB
Wei-Wen Cai - Houston TX, US

Assignee:

Seqwright, Inc. - Houston TX

International Classification:

G06F019/00
G01N033/48
C12Q001/68

US Classification:

702 20, 702 19, 435 6

Abstract:

A simplified strategy for sequencing large genomes has been developed. Clone-Array Pooled Shotgun Sequencing (CAPSS) is based upon pooling rows and columns of arrayed genomic clones, for shotgun library construction. Random sequences are accumulated and the data are assembled by sequential comparison of rows and columns, to resolve the sequence of clones at points of intersection. Compared to either a clone-by-clone approach or whole genome shotgun sequencing, CAPSS requires relatively few library constructions and only minimal computational power for a complete genome assembly. The strategy is suitable for sequencing large genomes for which there are no sequence-ready maps, but for which relatively high resolution STS maps and highly redundant BAC libraries are available. It is immediately applicable to the sequencing of mouse, rat, zebra fish and other important genomes, and can be managed in a cooperative fashion to take advantage of the distributed international DNA sequencing capacity.

US Patent:

7109178, Sep 19, 2006

Filed:

Jan 25, 2002

Appl. No.:

10/057050

Inventors:

Henry Ji - San Diego CA, US
Alan Greener - San Diego CA, US
Joseph A. Sorge - Wilson WY, US
John Bauer - San Diego CA, US
Richard Gibbs - Houston TX, US
Carsten-Peter Carstens - La Jolla CA, US

Assignee:

Stratagene California - La Jolla CA

International Classification:

A61K 31/711

US Classification:

514 44, 435462, 435455, 435 9141

Abstract:

The invention provides methods of covalently joining nucleic acid molecules and methods of molecular cloning. The methods provide either sequential or simultaneous ligation of flanking or vector nucleic acid molecules to nucleic acid insert molecules by topoisomerase and DNA ligase. The methods provide for directional and non-directional covalent joining and cloning of nucleic acid molecules.

US Patent:

20020032320, Mar 14, 2002

Filed:

Aug 14, 2001

Appl. No.:

09/880652

Inventors:

Kevin Burgess - Bryan TX, US
Richard Gibbs - Houston TX, US

Assignee:

The Texas A&M University System

International Classification:

C07H021/04
C07D311/82

US Classification:

536/023100, 549/216000, 549/223000

Abstract:

Fluorescent energy transfer cassettes that allow through bond energy transfer and have a succinimidyl ester functionality suitable for affecting them to biomolecules or provided and are applied to high throughput DNA sequencing.

US Patent:

20070072297, Mar 29, 2007

Filed:

Sep 18, 2006

Appl. No.:

11/523422

Inventors:

Henry Ji - San Diego CA, US
Alan Greener - San Diego CA, US
Joseph Sorge - Wilson WY, US
John Bauer - San Diego CA, US
Richard Gibbs - Houston TX, US
Carsten-Peter Carstens - La Jolla CA, US

International Classification:

C12N 15/09
C12P 19/34

US Classification:

435455000, 435091100

Abstract:

The invention provides methods of covalently joining nucleic acid molecules and methods of molecular cloning. The methods provide either sequential or simultaneous ligation of flanking or vector nucleic acid molecules to nucleic acid insert molecules by topoisomerase and DNA ligase. The methods provide for directional and non-directional covalent joining and cloning of nucleic acid molecules.

US Patent:

57285292, Mar 17, 1998

Filed:

Nov 6, 1995

Appl. No.:

8/553936

Inventors:

Michael L. Metzker - Houston TX
Richard A. Gibbs - Houston TX

Assignee:

Baylor College Of Medicine - Houston TX

International Classification:

C12Q 168
C12Q 170
C07H 2104
C12P 1934

US Classification:

435 6

Abstract:

Methods for the use of a class of dyes for improved DNA sequencing by the chain termination method of DNA sequencing, and internal labelling of polynucleotides by enzymatic incorporation of fluorescently-labeled ribonucleotides or deoxyribonucleotides are provided. A new class of dyes, BODIPY. RTM. fluorophores, has been described recently. The parent heterocyclic molecule of the BODIPY. RTM. fluorophores is a dipyrrometheneboron difluoride compound which is modified to create a broad class of spectrally-discriminating fluorophores. BODIPY. RTM. fluorophores have improved spectral characteristics compared to conventional fluorescein and rhodamine dyes. BODIPY. RTM. fluorophores have narrower band width, insensitivity to solvent or pH, and improved photostability, thus, BODIPY. RTM. fluorophores lead to improved DNA sequencing and/or detection in any method where electrophoresis and detection of DNA is required. Additionally, the spectral properties of the BODIPY. RTM.

US Patent:

59940638, Nov 30, 1999

Filed:

Mar 7, 1996

Appl. No.:

8/612036

Inventors:

Michael L. Metzker - Houston TX
Richard A. Gibbs - Houston TX

International Classification:

C12Q 168

US Classification:

435 6

Abstract:

The use of BODIPY. RTM. fluorophores for detecting a target nucleic acid is described. The parent heterocyclic molecule of the BODIPY. RTM. fluorophores is a dipyrrometheneboron difluoride compound which is modified to create a broad class of spectrally-discriminating fluorophores. The present invention provides oligonucleotides labelled with substituted 4,4-difluoro-4-bora-3A,4A-diaza-s-indacene (BODIPY. RTM. fluorophore) compounds for performing the Taqman assay.

US Patent:

56143869, Mar 25, 1997

Filed:

Jun 23, 1995

Appl. No.:

8/494216

Inventors:

Michael L. Metzker - Houston TX
Richard A. Gibbs - Houston TX

Assignee:

Baylor College of Medicine - Houston TX

International Classification:

C12P 1934
C12Q 168
C12Q 170
C07H 1904

US Classification:

435 911

Abstract:

Methods for the use of a class of dyes for improved DNA sequencing are provided. A new class of dyes, BODIPY. RTM. fluorophore, has been described recently. The parent heterocyclic molecule of the BODIPY. RTM. fluorophore is a dipyrrometheneboron difluoride compound which is modified to create a broad class of spectrally-discriminating fluorophores. The present invention provides methods for the use of BODIPY. RTM. fluorophore-labeled DNA. BODIPY. RTM. fluorophore have improved spectral characteristics compared to conventional fluorescein and rhodamine dyes. BODIPY. RTM. fluorophore have narrower band width, insensitivity to solvent or pH, and improved photostability, thus, BODIPY. RTM. fluorophores lead to improved DNA sequencing and/or detection in any method where electrophoresis and detection of DNA is required. Additionally, the spectral properties of the BODIPY. RTM.