Richard S Gordon

US Patent:

6406876, Jun 18, 2002

Filed:

Apr 26, 2000

Appl. No.:

09/558511

Inventors:

Richard K. Gordon - Potomac MD
Bhupendra P. Doctor - Potomac MD
Ashima Saxena - Fairfax VA
Shawn R. Feaster - Damascus MD
Donald Maxwell - Baltimore MD
Michelle Ross - Edgewood MD
David Lenz - Bel Air MD
Keith LeJeune - Pittsburgh PA
Alan Russell - Wexford PA

Assignee:

The United States of America as represented by the Secretary of the Army - Washington DC

International Classification:

C12Q 146

US Classification:

435 20, 435 19, 435 28, 435 18, 435 25, 4352891, 4352831, 4352871, 4352865, 435963, 435970

Abstract:

Methods, compositions and materials useful in the detection of organophosphorous and organosulfur compounds are disclosed. In particular, biosensors wherein a porous or a non-porous support having an enzyme immobilized upon or within are disclosed. The biosensors exhibit enzymatic stability at extreme temperatures and/or denaturing conditions, and similar kinetic characteristics of the soluble form of the enzymes utilized. The enzyme does not leach from the porous or non-porous support and the material retains enzymatic activity after prolonged storage. Differential biosensors are also disclosed.

US Patent:

6541230, Apr 1, 2003

Filed:

Apr 26, 2000

Appl. No.:

09/559396

Inventors:

Richard K. Gordon - Potomac MD
Bhupendra P. Doctor - Potomac MD

Assignee:

The United States of America as Represented by the Secretary of the Army - Washington DC

International Classification:

C12N 1118

US Classification:

435175, 435177, 435180, 435188, 4352625

Abstract:

A reusable sponge or foam made of a polymer such as polyurethane is prepared containing a plurality of different enzymes or a cross-linked complex of the plurality of enzymes for detoxification of a diverse array of hazardous chemicals such as organophosphorus and/or organosulfur compounds. The plurality of enzymes include enzymes selected from acetylcholinesterase, butyrylcholinesterase, triesterase, pseudocholinesterase, choline oxidase, peroxidase, organophosphate hydrolase, phosphotriesterase, paraoxonase and laccase. A preferred mixture of enzymes contains organophosphate hydrolase and acetylcholinesterase or butyrylcholinesterase. The sponge or foam may additionally contain carbon, an enzyme reactivation compound and/or an indicator for measuring capacity for detoxification. The indicator can be fluorescent, chemiluminescent or visible chromogen or an electrode, and be encapsulated in a liposome or crushable packet. The sponge or foam may be color-coded to indicate specific chemical detoxified, or to indicate enzymatic concentration, activity and/or remaining shelf-life.

US Patent:

6573244, Jun 3, 2003

Filed:

May 12, 2000

Appl. No.:

09/570022

Inventors:

Richard K. Gordon - Potomac MD
Debbie R. Moorad - Rockville MD
Bhupendra P. Doctor - Potomac MD
Gregory E. Garcia - Germantown MD

Assignee:

The United States of America as represented by the Secretary of the Army - Washington DC

International Classification:

A61K 3800

US Classification:

514 15, 536 231, 536 234, 435 691, 4241851

Abstract:

The compounds of the invention are generally described by the formula: or and the salts, esters, amides, and acyl forms thereof. Each position represented by a letter indicates a single amino acid residue: B is a basic or polar/large amino acid or a modified form thereof; X is a small or hydrophobic amino acid or a modified form thereof; X* is a small or polar/large amino acid or a modified form thereof; Z is a polar/large or hydrophobic amino acid or a modified form thereof; Z* is Proline or a polar/large or hydrophobic amino acid or a modified form thereof. As described below, one or more of the peptide linkages between the amino acid residues may be replaced by a peptide linkage mimic. These compounds may be used as molecular building blocks to create compounds that are optimized for inhibiting the protease activity of Botulinum B and tetanus toxins.

US Patent:

6642037, Nov 4, 2003

Filed:

Apr 26, 2000

Appl. No.:

09/558512

Inventors:

Richard K. Gordon - Potomac MD
Bhupendra P. Doctor - Potomac MD
Ashima Saxena - Fairfax VA
Shawn R. Feaster - Damascus MD
Donald Maxwell - Baltimore MD
Michelle Ross - Edgewood MD
David Lenz - Bel Air MD
Keith Lejeune - Pittsburgh PA
Alan Russell - Wexford PA

Assignee:

The United States of America as represented by the Secretary of the Army - Washington DC

International Classification:

C12N 1104

US Classification:

435182, 435180, 4352625

Abstract:

An enzymatically active reusable sponge or foam, capable of regeneration with oximes, made of a polymer such as polyurethane is prepared for detoxification of hazardous compounds such as organophosphorus and organosulfur compounds. The foam or sponge contains a plurality of enzymes including enzymes selected from acetylcholinesterase, butyrylcholinesterase, triesterase, pseudocholinesterase, organophosphate hydrase, phosphotriesterase, paraoxonase and organophosphorus and organosulfur hydrolyzing enzymes. The sponge or foam may additionally contain activated carbon and an enzyme reactivation compound. A kit can be formed containing the sponge or foam and the compound for enzyme reactivation. The enzymatically active foam or sponge may be prepared using a two chamber device where enzymes and prepolymer are passed from separate chambers into a static mixing stator and are subjected to low shear mixing and extrusion to form by the sponge or foam.

US Patent:

6713444, Mar 30, 2004

Filed:

May 12, 2000

Appl. No.:

09/570023

Inventors:

Gregory E. Garcia - Germantown MD
Richard K. Gordon - Potomac MD
Debbie R. Moorad - Rockville MD
Bhupendra P. Doctor - Potomac MD

Assignee:

The United States of America as represented by the Secretary of the Army - Washington DC

International Classification:

A61K 3800

US Classification:

514 2, 514 13, 514 21, 530324, 530326, 530333, 530344, 4242391, 424 91, 4352527

Abstract:

The compounds of the invention are generally described by the formula: and the salts, esters, amides, and acyl forms thereof. Up to 15 amino acids may be truncated from the N-terminus and up to 6 amino acids may be truncated from the C-terminus. Each position represented by a letter indicates a single amino acid residue wherein B is a basic or polar/large amino acid or a modified form thereof; X is a small or hydrophobic amino acid or a modified form thereof; X* is a small or polar/large amino acid or a modified form thereof; Z is a polar/large or hydrophobic amino acid or a modified form thereof; Z* is Proline or a polar/large or hydrophobic amino acid or a modified form thereof. These compounds may be used to inhibit the protease activity of Botulinum B and tetanus toxins.

US Patent:

6746850, Jun 8, 2004

Filed:

May 4, 2001

Appl. No.:

09/848370

Inventors:

Shawn R. Feaster - Damascus MD
Richard K. Gordon - Potomac MD
Bhupendra P. Doctor - Potomac MD

Assignee:

The United States of America as represented by the Secretary of the Army - Washington DC

International Classification:

C12Q 146

US Classification:

435 20

Abstract:

An assay for detecting, measuring, or monitoring the activity or concentration of at least two proteins that have similar or overlapping properties is disclosed. The assay comprises first determining the sensitivity coefficients of the substrates for each of the proteins in which the concentrations are to be determined. This method may be used for detecting, measuring, or monitoring the activity and concentration of AChE, BChE, or both in a test sample which test sample may be whole and unprocessed blood or tissue. Also disclosed are methods of using the assay to detect a subjects exposure to an agent which affects cholinesterase, determine the efficacy or progress of a treatment, determine the amount of protection provided against exposure to an agent which affects cholinesterase, or both, screen a subject for having a drug sensitivity or a particular disease, detect a change in red blood cell count of a subject, determine whether a candidate compound affects cholinesterase. Also disclosed are devices and kits for detecting, measuring, or monitoring the activities and concentrations of AChE, BChE, or both.

US Patent:

7235521, Jun 26, 2007

Filed:

May 15, 2000

Appl. No.:

09/979101

Inventors:

Richard K. Gordon - Potomac MD, US
Deborah R. Moorad - Silver Spring MD, US
Bhupendra P. Doctor - Potomac MD, US
Gregory E. Garcia - Silver Spring MD, US

Assignee:

United States of America as represented by the Secretary of the Army - Washington DC

International Classification:

A61K 38/00

US Classification:

514 2, 514 15, 530300, 435 71, 4353201, 536 235, 4242391

Abstract:

The compounds of the invention are generally described by the formula (1): BZ*BZ*X*QFXXXX, (2): BXXXXQFXXXX, or (3): BXBXZQFZXXXand the salts, esters, amides, and acyl forms thereof. Each position represented by a letter indicates a single amino acid residue: B is a basic or polar/large amino acid or a modified form thereof; X is a small or hydrophobic amino acid or a modified form thereof; X* is a small or polar/large amino acid or a modified form thereof; Z is a polar/large or hydrophobic amino acid or a modified form thereof; Z* is Proline or a polar/large or hydrophobic amino acid or a modified form thereof. As described below, one or more of the peptide linkages between the amino acid residues may be replaced by a peptide linkage mimic. These compounds may be used as molecular building blocks to create compounds that are optimized for inhibiting the protease activity of Botulinum B and tetanus toxins.

US Patent:

7375079, May 20, 2008

Filed:

Nov 4, 2005

Appl. No.:

11/268757

Inventors:

Richard K. Gordon - Potomac MD, US
Deborah R. Moorad - Silver Spring MD, US
Bhupendra P. Doctor - Potomac MD, US
Gregory E. Garcia - Silver Spring MD, US

Assignee:

The United States of America as represented by the Secretary of the Army - Washington DC

International Classification:

A61K 38/00

US Classification:

514 2, 514 15, 530300, 435 71, 4353201, 435325, 4242391, 536 235

Abstract:

The compounds of the invention are generally described by the formula:BZ*BZ*X*QFXXXX,   (1)BXXXXQFXXXX, or   (2)BZBXZQFZXXX   (3)and the salts, esters, amides, and acyl forms thereof. Each position represented by a letter indicates a single amino acid residue: B is a basic of polar/large amino acid or a modified form thereof; X is a small or hydrophobic amino acid or a modified form thereof; X* is a small or polar/large amino acid or a modified form thereof; Z is a polar/large or hydrophobic amino acid or a modified form thereof; Z* is Proline or a polar/large of hydrophobic amino acid or a modified form thereof. As described below, one or more of the peptide linkages between the amino acid residues may be replaced by a peptide linkage mimic. These compounds may be used as molecular building blocks to create compounds that are optimized for inhibiting the protease activity of Botulinum b and tetanus toxins.