Research and Diagnostic Systems, Inc Noncoml Research Orgnztn Mfg Medicinal/Botanicals Coml Physical Research Mfg Measure/Control Dvcs Mfg Relay/Indstl Control · Noncoml Research Orgnztn Mfg Medicinal/Botanicals Coml Physical Research Mfg Measure/Control Dvcs Relay/Indstl Control
Methods are provided for stabilization and purification of proteins produced in host cell systems. The methods provide for significant stabilization of any unfused non-bacterial protein that is produced in the same host cell that is producing an aggregate-forming fusion protein or any aggregate-forming protein.
Genetic Transformation Of Maize Cells By Electroporation Of Cells Pretreated With Pectin Degrading Enzymes
Richard A. Krzyzek - Edina MN Cheryl R. M. Laursen - St. Paul MN Paul C. Anderson - Minneapolis MN
Assignee:
DeKalb Genetics Corporation - DeKalb IL
International Classification:
C12N 1500 C12N 1505
US Classification:
4351723
Abstract:
The present invention provides a method to increase the susceptibility of cultured Zea mays cells to stable transformation with recombinant DNA via electroporation, by pretreating the Zea mays cells with certain pectin-degrading enzymes, so that the cells retain their ability to regenerate fertile, transgenic Zea mays plants containing the DNA which is also heritable.
Stable Transformation Of Maize Cells By Electroporation
Richard A. Krzyzek - Edina MN Cheryl R. M. Laursen - St. Paul MN Paul C. Anderson - Minneapolis MN
Assignee:
DeKalb Genetics Corporation - DeKalb IL
International Classification:
C12N 504 A01H 400
US Classification:
4352404
Abstract:
The present invention provides a method to increase the susceptibility of cultured Zea mays cells to stable transformation with recombinant DNA via electroporation, by pretreating the Zea mays cells with certain pectin-degrading enzymes, so that the cells retain their ability to regenerate fertile, transgenic Zea mays plants containing the DNA which is also heritable.
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