Richard F Senkpeil

US Patent:

20040053382, Mar 18, 2004

Filed:

Jun 9, 2003

Appl. No.:

10/433835

Inventors:

Richard Senkpeil - Lisle IL, US
David Pantaleone - Buffalo Grove IL, US
Paul Taylor - Arlington Heights IL, US

International Classification:

C12P007/42

US Classification:

435/146000, 435/280000

Abstract:

An economical and expedient method is disclosed for the preparation of -hydroxy-carboxylic acids or salts thereof in very high enantiomeric purity which comprises oxidizing a corresponding -amino-carboxylic acid or salt thereof using an amino acid deaminase followed by reducing the corresponding -keto-carboxylic acid or salt produced using a D- or L-lactate dehydrogenase in the combination with an electron donor and an enzyme/substrate system for recycling the electron donor. The resulting -hydroxy-carboxylic acids, hydrates, and salts thereof are valuable components and intermediates in the preparation of chiral compounds, especially pharmaceuticals. This invention also relates to the use of -amino-carboxylic acids, hydrates, and salts thereof and a coupled enzyme system in the production of -hydroxy-carboxylic acids, hydrates, and salts thereof.