Richard A Triggs

Richard Triggs

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Location:

United States

Richard Triggs

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Richard Triggs Mission Viejo, CA

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Work:

Rose, Mueller, & Greer Laboratories

Sep 2013 to 2000
Experiment Leader/Research Technician Walmart
Foothill Ranch, CA
Oct 2010 to Aug 2011
Customer Service/Cashier United States Marine Corps
Camp Pendleton, CA
Aug 2007 to Jun 2008
Gate Guard Supervisor/Military Policeman United States Marine Corps
Camp Pendleton, CA
Jun 2004 to Aug 2007
Infantry Squad Leader/Instructor

Education:

University of California, Irvine
Irvine, CA
2012 to 2014
BS in Biological Sciences Saddleback College
Mission Viejo, CA
2002 to 2012
AS in Biology

Skills:

LABORATORY - Biochemistry Spectrophotometry (DU640, DU800): Bradford assay; absorption spectrum scan; enzyme activity assay. Ammonium sulfate purification. Gel filtration chomotography. SDS PAGE. Protein crystallization. - Molecular Biology PCR. Gel electrophoresis. Restriction enzyme digest. DNA methylation. Plasmid prep (Qiagen): bacterial transformation; plasmid digestion and isolation. Primer design. RNA isolation. cDNA synthesis via RT-PCR. qPCR. - Virology/Immunology Splitting and maintenance of HeLa, HEK293, and Jurkat cells. Cell counting via hemocytometer. Plasmid transfection. DNA cloning. Viral infection. Plaque assay via -galactosidase staining. ELISA. Jurkat stimulation. Western blot. Phospho-tyrosine probing. FACS staining. - Evolutionary Biology Drosophila melanogaster. Fecundity. Mortality. Viability. Mate choice. Sex determination. Pure, single hybrid, and double hybrid crossing. - Basic lab skills Aseptic technique. Dilutions. Proficient in the use of micropipettes, vortex, centrifuge, glassware, burners, heaters, freezers, reagents containers and associated measuring devices. Familiar with laboratory safety guidelines. LEADERSHIP - Trained new interns in appropriate laboratory techniques and etiquette. - Supervised the proper completion of experiments in the lab. - Trained Marines in the accurate employment of the mortar system, rifleman tactics and military operations in urban terrain (MOUT). - Led a squad of Marines in training and combat operations. - Supervised 6 different entry control points aboard Marine Corps Base Camp Pendleton, CA as a military policeman in the Provost Marshalls Office (PMO). COMPUTER - Microsoft Windows, XP, 7, 8. - Microsoft Office 2010, 2013. - Microsoft Word 2010, 2013. - Microsoft Excel 2010, 2013. - Microsoft Powerpoint 2010, 2013 - PicTar, Primer3Plus, OligoCalc, PrimerBLAST.

Richard A Triggs

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Specialties:

Anesthesiology
Pediatrics

Education:

George Washington University (1964)