Robert T Bishop

US Patent:

6410264, Jun 25, 2002

Filed:

Jul 31, 1998

Appl. No.:

09/127578

Inventors:

Kenneth A. Crawford - Alameda CA
Robert Bishop - San Francisco CA

Assignee:

Chiron Corporation - Emeryville CA

International Classification:

C12Q 168

US Classification:

435 691, 435 5, 435 6, 435 911, 4351723, 4353201, 935 72, 935 33

Abstract:

Regulatory nucleotide sequences for a novel gene, designated PpSEC10 gene, and the nucleotide sequences and respective amino acid sequences for the secretion leader and the mature Sec10p protein components of the precursor polypeptide encoded by this novel gene are provided. These compositions are useful in methods for expression and secretion of proteins when assembled in proper reading frame, individually or in combination, within a DNA construct that further comprises a nucleotide sequence encoding a protein of interest. Vectors comprising the DNA constructs of the invention can be used to transform a yeast host cell, which can then be cultured to obtain the secreted protein of interest. Kits useful in this method and in methods of detection of the Sec10p protein using antibodies are also disclosed.

US Patent:

6605450, Aug 12, 2003

Filed:

May 24, 2001

Appl. No.:

09/866274

Inventors:

Robert J. Bishop - Berkeley CA
Kenneth A. Crawford - Alameda CA
Margaret M. Worden - Kensington CA

Assignee:

Chiron Corporation - Emeryville CA

International Classification:

C12P 2102

US Classification:

435 691, 4353201, 435483, 536 241

Abstract:

Methods and compositions for the expression of nucleotide sequences are provided. Compositions comprise the nucleic acid sequences of the transcriptional regulatory region of the PpSEC10 gene of Methods are provided to chemically regulate the PpSEC10 transcriptional control region by modulating the iron concentration in the culturing medium. The methods find use in regulating expression of nucleotide sequences. Furthermore, the methods of the invention can be used to regulate polypeptide expression, more particularly in regulating heterologous polypeptide expression, particularly using a yeast host cell as the expression system.

US Patent:

20030013154, Jan 16, 2003

Filed:

Jun 25, 2002

Appl. No.:

10/179046

Inventors:

Kenneth Crawford - Alameda CA, US
Isabel Zaror - Orinda CA, US
Robert Bishop - San Francisco CA, US
Michael Innis - Moraga CA, US

Assignee:

Chiron Corporation - Emeryville CA

International Classification:

C12P021/02
C12N009/50
C12N001/18
C07H021/04
C12N015/74

US Classification:

435/069100, 435/254200, 435/219000, 435/320100, 536/023200

Abstract:

Polynucleotides, vectors and host cells comprising a polynucleotide having a fragment of a leader sequence and a second nucleotide sequence that encodes a polypeptide heterologous to the leader sequence, wherein the leader sequence fragment is sufficient for secretion and comprises an amino acid sequence that comprises at least about 70% sequence identity to the leader sequence of killer toxin, wherein the heterologous polypeptide is not naturally contiguous to the leader sequence, and wherein upon expression of the polynucleotide molecule in a host cell suitable for expression thereof, the heterologous polypeptide is produced that is free of additional N-terminal amino acids.

US Patent:

54085764, Apr 18, 1995

Filed:

Oct 28, 1992

Appl. No.:

7/967434

Inventors:

Robert A. Bishop - Dallas TX

International Classification:

H05B 100

US Classification:

392470

Abstract:

A cabinet structure is specially adapted for receiving a plurality of IV fluid bags. When IV bags are placed inside this cabinet structure, the user turns on a power switch to begin a very specific and controlled heating cycle. A temperature indicator on the front of the cabinet allows the user to ascertain when the desired temperature has been reached. When an IV bag is desired for use, it is removed directly from the side of the cabinet through an opening approximately the size of the IV bag. A temperature sensor inside the bag compartment allows for automatic regulation of the temperature of a pad of heating filaments located along the back face of the inside the cabinet. The heating elements are covered by a rubber layer to protect the bags from melting. The cabinet structure can be fastened to any IV pole, hung on the side of an anesthesia cart or be mounted directly to a wall.

US Patent:

61070572, Aug 22, 2000

Filed:

Feb 24, 1998

Appl. No.:

9/029267

Inventors:

Kenneth Crawford - Alameda CA
Isabel Zaror - Orinda CA
Robert J. Bishop - San Francisco CA
Michael A. Innis - Moraga CA

Assignee:

Chiron Corporation - Emeryville CA

International Classification:

C12P 2100
C12N 510
C12N 111
C12N 1531

US Classification:

435 691

Abstract:

Polynucleotides, vectors, and host cells comprising a polynucleotide having a fragment of a leader sequence and a second nucleotide sequence that encodes a polypeptide heterologous to the leader sequence, wherein the leader sequence fragment is sufficient for secretion and comprises an amino acid sequence that comprises at least about 70% sequence identity to the leader sequence of Pichia acaciae killer toxin, wherein the heterologous polypeptide is not naturally contiguous to the leader sequence, and wherein upon expression of the polynucleotide molecule in a host cell suitable for expression thereof, the heterologous polypeptide is produced that is free of additional N-terminal amino acids.

US Patent:

59242892, Jul 20, 1999

Filed:

Jul 1, 1997

Appl. No.:

8/886576

Inventors:

Robert A. Bishop - Plano TX

Assignee:

Medical Products, Inc. - Chantilly VA

International Classification:

F25B 2102

US Classification:

62 361

Abstract:

A temperature controlled cabinet system and method enables medical items associated with particular temperature ranges to be stored and maintained within their associated temperature ranges in cabinets disposed within ambulances and other medical vehicles. The system includes a temperature sensor for measuring the cabinet interior temperature, a controller assembly for controlling system operation and two heat pumps disposed in the cabinet walls to heat or cool the cabinet interior. Alternatively, a single heat pump may be utilized to heat or cool the cabinet interior. The controller assembly includes a control console for displaying the actual cabinet temperature and entering a desired temperature range into the system. The heat pumps include a pair of heat sinks disposed about a thermoelectric device (e. g. , a Peltier chip) that heats one heat sink, while cooling the other heat sink based on voltage polarity or current flow direction applied to the device.

US Patent:

61184918, Sep 12, 2000

Filed:

Feb 20, 1997

Appl. No.:

8/803504

Inventors:

Scarlett Wu - Hillsborough CA
Darren D Neuman - San Jose CA
Robert F Bishop - Morgan Hill CA

Assignee:

LSI Logic Corporation - Milpitas CA

International Classification:

H04N 508

US Classification:

348526

Abstract:

A system and method for synchronizing a decoded, interlaced-field data stream with an interlaced field display. A system for displaying an MPEG encoded data stream includes an MPEG decoder which converts the encoded data stream into a sequence of frames. Each frame has an associated top field, bottom field, top-field-first flag, and repeat-first-field flag. The system also includes a display processor which receives the flags and determines a field display sequence for each frame which conforms to an overall display sequence which strictly alternates between top and bottom fields. This strict alternation in enforced even when the decoded field sequence does not adhere to a strict alternation. The system achieves this result with a worst-case temporal distortion of one field by inserting or omitting a 3:2 pulldown frame at each broken alternation point.