Robert W Bonner

US Patent:

7709047, May 4, 2010

Filed:

Jul 23, 2003

Appl. No.:

10/543218

Inventors:

Michael R. Emmert-Buck - Easton MD, US
Michael Anthony Tangrea - Odenton MD, US
Robert F. Bonner - Washington DC, US
Rodrigo Chuaqui - North Potomac MD, US
Thomas J. Pohida - Monrovia MD, US

Assignee:

The United States of America as represented by the Secretary of the Department of Health and Human Services - Washington DC

International Classification:

B05D 1/00

US Classification:

427 211, 428346, 428352, 356 36, 356 38, 356402, 356417, 4352883, 4352884

Abstract:

A method of removing a target from a biological sample which involves placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere it to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target). The immunoreagent can alter the transfer surface directly (for example with a heat generating enzyme carried by the immunoreagent), or indirectly (for example by changing a characteristic of the target).

US Patent:

8597715, Dec 3, 2013

Filed:

Apr 2, 2010

Appl. No.:

12/753566

Inventors:

Michael R. Emmert-Buck - Easton MD, US
Michael Anthony Tangrea - Odenton MD, US
Robert F. Bonner - Washington DC, US
Rodrigo Chuaqui - North Potomac MD, US
Thomas J. Pohida - Monrovia MD, US

Assignee:

The United States of America as represented by the Secretary of the Department of Health and Human Services - Washington DC

International Classification:

B05D 1/00

US Classification:

427 211, 428346, 428352, 356 36, 356 38, 356402, 356417, 4352883, 4352884

Abstract:

A method of removing a target from a biological sample which involves placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere it to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target). The immunoreagent can alter the transfer surface directly (for example with a heat generating enzyme carried by the immunoreagent), or indirectly (for example by changing a characteristic of the target).