Robert T. Buckler - Edwardsburg MI Robert P. Hatch - Elkhart IN
Assignee:
Miles Inc. - Elkhart IN
International Classification:
C12Q 128
US Classification:
435 28
Abstract:
Fluorogenic indicator compounds responsive to the presence of hydrogen peroxide and methods and test compositions for determining hydrogen peroxide in a test sample. Preferred indicator compounds are 3,4-dihydrocoumarin derivatives and 3,4-dihydro-2-quinolone derivatives, especially those having a dialkylamino group at the 7-position. Such particularly preferred compounds are of the formula: ##STR1## wherein R. sup. 1 is hydrogen, cyano, or --COOR. sup. 6, --CONHR. sup. 6 or --CON(R. sup. 6). sub. 2 where R. sup. 6 is hydrogen, alkyl, alkenyl, or aryl; R. sup. 3 and R. sup. 4 are lower alkyl; and Y is. dbd. 0 or. dbd. N--R. sup. 5 where R. sup. 5 is hydrogen or lower alkyl. The indicator compounds yield highly fluorescent products upon oxidation by hydrogen peroxide in the presence of a peroxidatively active substance and are useful in analytical systems which generate hydrogen peroxide in response to an analyte under determination in a test sample.
Robert T. Buckler - Edwardsburg MI Robert J. Carrico - Elkhart IN
Assignee:
Miles Laboratories, Inc. - Elkhart IN
International Classification:
C07G 700 A61K 3900
US Classification:
260112B
Abstract:
A propranolol immunogen comprising an unsubstituted hydroxypropylamine precursor of propranolol coupled through a bis-imidate linking group to an immunogenic carrier material. The immunogen is useful to stimulate the production of antibodies which recognize propranolol and can perform as reagents in immunoassays to determine propranolol.
Robert T. Buckler - Edwardsburg MI Robert P. Hatch - Elkhart IN
Assignee:
Miles Inc. - Elkhart IN
International Classification:
C07D21520 C07D31102
US Classification:
546155
Abstract:
Optical indicator chalcogen (selenide or sulfide) compounds responsive to oxidants, e. g. , hydrogen peroxide, and methods for preparing and using such indicators. Upon oxidation the resulting intermediate undergoes spontaneous elimination of the chalcogen residue to yield a signal compound which provides an optical signal such as fluorescence. Preferred fluorogenic indicator compounds are 3-chalcogen-3,4-dihydrocoumarin derivatives and 3-chalcogen-3,4-dihydro-2-quinolone derivatives. Such indicator compounds provide highly fluorescent products upon oxidation by hydrogen peroxide and are useful in analytical systems which generate hydrogen peroxide in response to the analyte under determination.
3-Methylene Flavanones And 3-Methylene Chromanones
Robert T. Buckler - Edwardsburg MI Frederick E. Ward - Elkhart IN David L. Garling - Elkhart IN
Assignee:
Miles Laboratories, Inc. - Elkhart IN
International Classification:
C07D40504 A61K 3135 A61K 3144 C07D31128
US Classification:
424263
Abstract:
Compounds which are 3-methylene flavanones and 3-methylene chromanones having activity against microorganisms are disclosed. The compounds are represented by the general structural formula: ##STR1## wherein: R. sup. 1 is a member selected from the group consisting of hydrogen, Br, CH. sub. 3 and OCH. sub. 3 ; R. sup. 2 is selected from the group consisting of hydrogen and ##STR2## wherein R. sup. 4 is a member selected from the group consisting of hydrogen, Br, Cl, CH. sub. 3, OCH. sub. 3, NO. sub. 2, N(CH. sub. 3). sub. 3 and CN; R. sup. 5 is selected from the group consisting of hydrogen and Cl, with the proviso that when R. sup. 5 is Cl, R. sup. 4 is hydrogen or Cl; and R. sup. 3 is selected from the group consisting of hydrogen, phenyl, 2-thienyl, 4-pyridyl and naphthyl, with the proviso that when R. sup. 3 is naphthyl, R. sup. 1 and R. sup.
Chemiluminescent Phthalhydrazide-Labeled Protein And Polypeptide Conjugates
Robert T. Buckler - Edwardsburg MI Hartmut R. Schroeder - Elkhart IN
Assignee:
Miles Laboratories, Inc. - Elkhart IN
International Classification:
C07G 700
US Classification:
260112B
Abstract:
Chemiluminescent-labeled conjugates of the formula: ##STR1## wherein one of R. sup. 1 and R. sup. 2 is hydrogen and the other is --NR. sup. 3 R. sup. 4 ; R. sup. 3 is hydrogen or straight chain alkyl containing 1-4 carbon atoms and R. sup. 4 is L(CO)--HN--(CH. sub. 2). sub. n wherein n=2-8 and L(CO)-- is an antigenic protein or polypeptide bound through an amide bond. The labeled conjugates are useful as reagents in specific binding assays for determining antigenic proteins or polypeptides, or their binding partners, in liquid media.
Phenyl- And (Substituted)-Phenyl-1,2,3-Triazole-Alkanoic And -Alkenoic Acids
Disclosed are prostaglandin analogues having the structural formula, ##STR1## in which: T is selected from the group consisting of carboxyl, alkoxycarbonyl or cyano; M is selected from the group consisting of carbonyl, R-hydroxymethylene or S-hydroxymethylene; L is selected from the group consisting of methylene or methine, provided L is methine only if J is methine; J is selected from the group consisting of methylene, ethylene, R-hydroxymethylene, S-hydroxymethylene or methine, provided J is methine only if L is methine; W is selected from the group consisting of ##STR2## T. sub. 1 and T. sub. 2 are attached to adjacent carbon atoms; T. sub. 1 is selected from the group consisting of hydrogen or phenyl, provided T. sub. 1 is phenyl only if T. sub. 2 is lower alkyl; T. sub.
Jeffrey L. Benovic - Durham NC Robert T. Buckler - Edwardsburg MI John F. Burd - Elkhart IN Thomas M. Li - Elkhart IN
Assignee:
Miles Laboratories, Inc. - Elkhart IN
International Classification:
C07D23954
US Classification:
544311
Abstract:
Intermediates in the preparation of 9-position derivatives of theophylline useful in synthesizing theophylline immunogens and labeled conjugates. The labeled conjugates and the antibodies produced against the immunogens are particularly useful in homogeneous nonradioisotopic immunoassay for measuring theophylline in biological fluids such as serum.