Robert L Diller

Address:

4050 E Appleby Dr, Gilbert, AZ 85298

Phone:

4809885209

VIN:

5FNYF28767B021302

Make:

HONDA

Model:

PILOT

Year:

2007

US Patent:

20220305061, Sep 29, 2022

Filed:

Jun 16, 2022

Appl. No.:

17/842156

Inventors:

- Flagstaff AZ, US
Robert B. Diller - Phoenix AZ, US
Robert G. Audet - Dracut MA, US
Eric T. Lee - Phoenix AZ, US
Andrew T. Vo - Phoenix AZ, US

Assignee:

AXOLOTL BIOLOGIX, INC. - Flagstaff AZ

International Classification:

A61K 35/50
C12N 5/073
A61K 38/17
A61P 19/04
A61P 17/02
A61K 38/18
A61K 38/19
A61K 38/45
A61K 38/48
A61P 19/02

Abstract:

A method for making an acellular human amnion-derived composition configured for therapeutic use is disclosed and generally includes the steps: obtaining amniotic membrane tissue; testing the amniotic membrane tissue for pathogens; washing the amniotic membrane tissue; manually removing blood-containing chorion tissue from the amniotic membrane tissue decellularizing the amniotic membrane tissue with xeno-free enzymes; collecting amniotic cells from the decellularized amniotic membrane tissue; seeding the amniotic cells for culture into xeno-free media formulated for mesenchymal stem cells; growing the amniotic cells to a specified confluency; collecting conditioned media; and freezing the collected conditioned media; wherein the method further includes irradiating the conditioned media.