Robert D Fredrickson

US Patent:

50473247, Sep 10, 1991

Filed:

Sep 16, 1987

Appl. No.:

7/095621

Inventors:

Robert A. Fredrickson - Elkhart IN

Assignee:

Miles Inc. - Elkhart IN

International Classification:

G01N 33535
G01N 33563

US Classification:

435 79

Abstract:

A method for obtaining a substantially pure preparation of an enzyme-antibody conjugate having an enzyme component covalently coupled to a predetermined number of an antibody component where the molecular weight of the enzyme component is substantially greater than the molecular weight of the antibody component. The method involves the electrophoretic separation of the desired enzyme-antibody conjugate from an enzyme-antibody conjugate reaction mixture comprising, as separately migratable species, at least a free enzyme component and a poplulation of enzyme-antibody conjugates comprising one or more of the enzyme component convalently coupled to one or more of the antibody component. The resulting conjugate preparations are substantially pure and are therefore particularly useful as labeled reagents in immunometric assays.

US Patent:

48144339, Mar 21, 1989

Filed:

Sep 16, 1987

Appl. No.:

7/097115

Inventors:

Robert A. Fredrickson - Elkhart IN

Assignee:

Miles Inc. - Elkhart IN

International Classification:

A61K 39395

US Classification:

530388

Abstract:

A method for obtaining a papain-free F(ab'). sub. 2 antibody fragment preparation derived from papain digested whole IgG antibody employing an antibody to papain for binding to and removing papain-associated digestion products from the preparation. The method is particularly useful for digesting whole IgG antibodies which are otherwise sensitive to pepsin digestion to provide F(ab'). sub. 2 antibody fragments from which Fab' antibody fragments can be obtained having available free sulfhydryl groups in the hinge region.