Robert J Gargiulo

US Patent:

42751535, Jun 23, 1981

Filed:

Aug 3, 1978

Appl. No.:

5/930826

Inventors:

Robert J. Gargiulo - Miami FL
Gary A. Mitchell - Miami FL
Patricia M. Hudson - Miami FL
Sharon P. Pochron - Miami FL
Rolf M. Huseby - Miami FL
Robert E. Smith - Livermore CA

Assignee:

American Hospital Supply Corporation - Evanston IL

International Classification:

C12Q 136
C12Q 138
C12Q 156
C07G 700

US Classification:

435 13

Abstract:

Fluorogenic substrates for proteolytic enzymes having the formula: ##STR1## or acid salts thereof wherein: R. sub. 1 is hydrogen-L, hydrogen-D, benzoyl, benzenesulfonyl, glutaryl, pyroglutamyl, carbobenzoxy, D-serine, or carbobenzoxy-serine; R. sub. 2 is hydrogen, phenyl, a straight, branched or cyclic alkyl having 1 to 4 carbons, or propionic acid; R. sub. 3 is hydrogen, straight or branched or cyclic alkyl having 1 to 4 carbons, 4-aminobutane, or 3-guanidylpropane; R. sub. 4 is methyl, 4-aminobutane, or 3-guanidylpropane; R. sub. 5 is a fluorogenic moiety, severable from said compound by a proteolytic enzyme and having different fluorescent property when severed from said compound than when forming part of said compound. The enzymes, when reacting with the substrate, remove the fluorogenic group R. sub. 5 producing an increase in its fluorescence.

US Patent:

41027477, Jul 25, 1978

Filed:

Jul 28, 1977

Appl. No.:

5/820072

Inventors:

Richard Cornelius Driscoll - Lake Forest IL
Robert James Gargiulo - Miami FL
Joseph L. Giegel - Miami FL

Assignee:

American Hospital Supply Corporation - Evanston IL

International Classification:

G01N 3114

US Classification:

1951035C

Abstract:

A method for the determination of amylase, based on the cleavage of colorimetric, ultraviolet absorbing or fluorometric substances, commonly referred to as chromogenic substances as p-nitrophenol derivatives of oligosaccharides of chain length 4-10 glucose units. Oligosaccharides of this chain length are resistant to cleavage by. alpha. and/or. beta. -glucosidase and contain endo-. alpha. -1,4 linkages which are required for amylase activity. Cleavage of the. alpha. -1,4 bonds by amylase produces smaller fragments which are acted upon by. alpha. -glucosidase and/or. beta. -glucosidase to liberate a chromophore. The rate of appearance of the chromophore is proportional to amylase activity and lends itself to either rate or endpoint determinations. The qualitative or quantitative measurement of amylase concentrations in a sample is useful in medical diagnosis.

US Patent:

43361862, Jun 22, 1982

Filed:

Feb 9, 1981

Appl. No.:

6/232942

Inventors:

Robert J. Gargiulo - Miami FL
Gary A. Mitchell - Miami FL
Patricia M. Hudson - Miami FL
Sharon P. Pochron - Miami FL
Rolf M. Huseby - Miami FL
Robert E. Smith - Livermore CA

International Classification:

C07C10352

US Classification:

2601125R

Abstract:

Fluorogenic substrates for proteolytic enzymes having the formula: ##STR1## or acid salts thereof wherein: R. sub. 1 is hydrogen-L, hydrogen-D, benzoyl, benzenesulfonyl, glutaryl, pyroglutamyl, carbobenzoxy, D-serine, or carbobenzoxy-serine; R. sub. 2 is hydrogen, phenyl, a straight, branched or cyclic alkyl having 1 to 4 carbons, or propionic acid; R. sub. 3 is hydrogen, straight or branched or cyclic alkyl having 1 to 4 carbons, 4-aminobutane, or 3-guanidylpropane; R. sub. 4 is methyl, 4-aminobutane, or 3-guanidylpropane; R. sub. 5 is a fluorogenic moiety, severable from said compound by a proteolytic enzyme and having different fluorescent property when severed from said compound than when forming part of said compound. The enzymes, when reacting with the substrate, remove the fluorogenic group R. sub. 5 producing an increase in its fluorescence.

US Patent:

41674499, Sep 11, 1979

Filed:

Aug 5, 1977

Appl. No.:

5/822057

Inventors:

Robert J. Gargiulo - Miami FL
Richard C. Driscoll - Lake Forest IL

Assignee:

American Hospital Supply Corporation - Evanston IL

International Classification:

G01N 3114
G01N 3316

US Classification:

435 16

Abstract:

Transferase and protease activity in homogenates and biological solutions is fluorometrically determined at wavelengths corresponding generally to those used for fluorometric NADH-linked determinations by utilizing novel substrate compositions consisting essentially of certain fluorogenic 5-aminoisophthalic acid derivatives coupled to amino acid constituents specific to the transferases and proteases under investigation.