Robert J Huebner

US Patent:

6379675, Apr 30, 2002

Filed:

Jan 19, 1996

Appl. No.:

08/588621

Inventors:

Robert S. Becker - Henryville PA
Robert C. Huebner - Stroudsburg PA
Maryann Gray - Bartonsville PA
Karen S. Biscardi - South Sterling PA
Lorne F. Erdile - Tassin la Demi Lune, FR
Bruno Guy - Lyons, FR

Assignee:

Connaught Laboratories, Inc. - Swiftwater PA

International Classification:

A61K 3902

US Classification:

4242341, 4241921, 4241931, 42419711, 4242371, 4242441, 530350, 435 691

Abstract:

Immunological compositions and methods for making and using them. The compositions contain at least one antigen and at least one lipoprotein and optionally an adjuvant. The lipoprotein can itself be antigenic or immunogenic. The antigen can be influenza HA and the lipoprotein a recombinantly expressed product having an OspA leader for lipidation and PspA for the protein portion. The antigen can be OspC and the lipoprotein OspA. The components of the composition are co-administered. A potentiated immunological response is obtained by the compositions and methods.

US Patent:

6451769, Sep 17, 2002

Filed:

Oct 15, 1998

Appl. No.:

09/173053

Inventors:

Robert C. Huebner - Stroudsburg PA
Jon A. Norman - San Diego CA
Xiaowu Liang - La Jolla CA
Kristin R. Carner - San Diego CA
Alan G. Barbour - Irvine CA
Catherine J. Luke - Irvine CA

Assignee:

Pasteur Merieux Serums et Vaccins - Lyons
Vical, Inc. - San Diego CA
The University of Texas System - Austin TX

International Classification:

A61K 3170

US Classification:

514 44, 435 914, 435 9141, 4353201, 435455

Abstract:

Disclosed is a vaccine against Lyme Disease or its causative agent (sensu stricto or sensu lato) containing a plasmid a DNA encoding a promoter for driving expression in a mammalian cell, DNA encoding a leader peptide for facilitating secretion/release of a prokaryotic protein sequence from a mammalian cell, a DNA encoding Borrelia OspA or OspB, and a DNA encoding a terminator. Disclosed too is an immunogenic composition against Lyme Disease or its causative agent (sensu stricto or sensu lato) containing a plasmid comprising a DNA encoding a promoter for driving expression in a mammalian cell, DNA encoding a leader peptide for facilitating secretion/release of a prokaryotic protein sequence from a mammalian cell, a DNA encoding a Borrelia OspC, and a DNA encoding a terminator. And, methods for making and using such vaccines and the immunogenic composition are also disclosed.

US Patent:

6538118, Mar 25, 2003

Filed:

Apr 28, 1998

Appl. No.:

09/067453

Inventors:

Robert C. Huebner - Stroudsburg PA
Lorne F. Erdile - Stroudsburg PA
Robert S. Becker - Henryville PA
Maryann B. Gray - Bartonsville PA
Derek J. Pyle - East Stroudsburg PA

Assignee:

Connaught Laboratories, Inc. - Swiftwater PA

International Classification:

C07H 2104

US Classification:

536 231, 536 234, 536 237, 435 691, 435 697, 4351723, 4353201

Abstract:

Heterologous lipidated proteins formed recombinantly are disclosed and claimed. The expression system can be. The heterologous lipidated protein has a leader sequence which does not naturally occur with the protein portion of the lipidated protein. The lipidated protein can have the Borrelia OspA leader sequence. The protein portion can be OspC, PspA, UreA, Ure B, or a fragment thereof. Methods and compositions for forming and employing the proteins are also disclosed and claimed.

US Patent:

6984385, Jan 10, 2006

Filed:

Mar 12, 2002

Appl. No.:

10/096687

Inventors:

Robert S. Becker - Henryville PA, US
Robert C. Huebner - Stroudsburg PA, US
Maryann Gray - Bartonsville PA, US
Karen S. Biscardi - South Sterling PA, US
Lorne F. Erdile - Tassin la Demi Lune, FR
Bruno Guy - Lyons, FR

Assignee:

Connaught Laboratories, Inc. - Swiftwater PA

International Classification:

A61K 39/00

US Classification:

4241921, 4242341, 4241931, 42419711, 4242001, 4242011, 4242031, 4242371, 4242441, 530350, 435 691, 435 693, 435 697, 435 711

Abstract:

Immunological compositions and methods for making and using them. The compositions contain at least one antigen and at least one lipoprotein and optionally an adjuvant. The lipoprotein can itself be antigenic or immunogenic. The antigen can be influenza HA and the lipoprotein a recombinantly expressed product having an OspA leader for lipidation and PspA for the protein portion. The antigen can be OspC and the lipoprotein OspA. The components of the composition are co-administered. A potentiated immunological response is obtained by the compositions and methods.

US Patent:

7235243, Jun 26, 2007

Filed:

Jul 1, 2005

Appl. No.:

11/173364

Inventors:

Robert S. Becker - Henryville PA, US
Robert C. Huebner - Stroudsburg PA, US
Maryann Gray - Bartonsville PA, US
Karen S. Biscardi - South Sterling PA, US
Lorne F. Erdile - Tassin la Demi Lune, FR
Bruno Guy - Lyons, FR

Assignee:

Connaught Laboratories, Inc. - Swiftwater PA

International Classification:

A61K 39/00

US Classification:

4241921, 4241931, 42419711, 4242001, 4242011, 4242031, 4242341, 4242371, 4242441, 435 691, 435 693, 435 697, 435 711, 530350

Abstract:

Immununological compositions and methods for making and using them. The compositions contain at least one antigen and at least one lipoprotein and optionally an adjuvant. The lipoprotein can itself be antigenic or immunogenic. The antigen can be influenza HA and the lipoprotein a recombinantly expressed product having an OspA leader for lipidation and PspA for the protein portion. The antigen can be OspC and the lipoprotein OspA. The components of the composition are co-administered. A potentiated immunological response is obtained by the compositions and methods.

US Patent:

20050171343, Aug 4, 2005

Filed:

Feb 6, 2003

Appl. No.:

10/359435

Inventors:

Robert Huebner - Stroudsburg PA, US
Lorne Erdile - Stroudsburg PA, US
Donald Warakomski - Tannersville PA, US
Robert Becker - Henryville PA, US
Maryann Gray - Bartonsville PA, US
Derek Pyle - East Stroudsburg PA, US

International Classification:

C12Q001/68
C07H021/04
C12N015/09
A61K039/02
C07K014/195
C12N001/21

US Classification:

536023700, 424190100, 435006000, 435069300, 435252300, 435320100, 530359000

Abstract:

Heterologous lipidated proteins formed recombinantly are disclosed and claimed. The expression system can be The heterologous lipidated protein has a leader sequence which does not naturally occur with the protein portion of the lipidated protein. The lipidated protein can have the OspA leader sequence. The protein portion can be OspC, PspA, UreA, Ure B, or a fragment thereof. Methods and compositions for forming and employing the proteins are also disclosed and claimed.

US Patent:

56120370, Mar 18, 1997

Filed:

Jul 26, 1994

Appl. No.:

8/280463

Inventors:

Robert C. Huebner - Bartonsville PA
Maurice W. Harmon - Tannersville PA

Assignee:

Connaught Laboratories, Inc. - Swiftwater PA

International Classification:

A61K 39145
A61K 39385

US Classification:

4241941

Abstract:

Conjugates of HA protein of influenza virus suitable for formulation as a vaccine for obtaining a strong immune response to the HA protein are formed by separating whole HA protein from the influenza virus by detergent extraction or by providing whole HA protein by recombinant procedure, treating the HA protein with hydroxylamine to form free sulfhydryl groups in the cytoplasmic domain of the protein, and cross-linking the free sulfhydryl group-containing HA protein to itself using a bis-maleimide linker or to a maleimide-modified diphtheria toxoid, tetanus toxoid or influenza NP protein or other carrier molecule. The procedure is applicable to other proteins which can be separated from a cellular material, such as a virus, and which contain thioester bonds convertible to sulfhydryl groups.

US Patent:

56860781, Nov 11, 1997

Filed:

Feb 8, 1995

Appl. No.:

8/385586

Inventors:

Robert S. Becker - Henryville PA
Laura Ferguson - Bethlehem PA
Lorne Erdile - Stroudsberg PA
Maurice W. Harmon - Tannersville PA
Robert Huebner - Bartonsville PA

Assignee:

Connaught Laboratories, Inc. - Swiftwater PA

International Classification:

A61K 39145
A61K 3912
A61K 3900
A61K 39002

US Classification:

4242091

Abstract:

Animals, including humans, are immunized by antigens, for example, the HA antigen of influenza, by first administering to a naive animal a normally strongly-immunogenic form of the antigen, for example, inactiviated or attenuated whole cell virus and subsequently administering a normally weakly-immunogenic isolated and purified viral antigen, to achieve an enhanced immune response to the purified viral antigen.