Robert L Matson

US Patent:

6841379, Jan 11, 2005

Filed:

May 15, 2002

Appl. No.:

10/146004

Inventors:

Robert S. Matson - Orange CA, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

C12M 134

US Classification:

4352872, 4352884, 435817, 4351731, 20440301, 20440303

Abstract:

A conductive microplate device for the detection of target biomolecules in a sample is described. The microplate comprises an assembly of a porous substrate and a conductive layer, wherein the assembly is sealed into bottom of at least some wells of the microplate. The porous substrate has a top surface and a bottom surface. The top surface comprises a plurality of covalently attached probe biomolecules. The covalently attached probe biomolecules are reactive with the target biomolecules contained in the sample. The conductive layer, which is attached to the bottom surface of the porous substrate, is adapted to receive voltage. Microplates of the present invention can be easily adapted for use with robotic workstations. Accordingly, in one embodiment, the power supply is incorporated into a robotic arm tool for fast microplate processing.

US Patent:

6861214, Mar 1, 2005

Filed:

Oct 23, 2000

Appl. No.:

09/694701

Inventors:

Jang B. Rampal - Yorba Linda CA, US
Robert S. Matson - Orange CA, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

C12Q001/68
A61L009/00
B32B027/04
A62B007/08
B01J019/08

US Classification:

435 6, 422 5, 422 50, 422 61, 422 71, 422 88, 422 99, 422122, 422131, 422186

Abstract:

An assay article for detection biopolymers contained in a sample is described. The assay article includes a substrate and a biopolymer directly adsorbed on the surface of the substrate. A plurality of biopolymers may be adsorbed on the surface of the substrate to form an array. Also disclosed is a method of making the assay article. In the preferred method, an aminated polypropylene substrate is used. A biopolymer is contacted with the aminated substrate under a condition sufficient for direct adsorption of the biopolymer on the surface of the substrate. A method of detecting a target biopolymer contained in a sample is also disclosed. In this method, a substrate is contacted with either a probe or target biopolymer under a condition sufficient for a direct adsorption of either the probe or target biopolymer on the substrate to form a probe assay article or a target assay article. Then, the probe assay article is contacted with the target biopolymer, or the target assay article is contacted with the probe biopolymer under a condition that allows the formation of a probe-target complex. Finally, the complex is detected and the presence of the complex is used as a measurement for the presence or the amount of the biopolymer target contained in the sample.

US Patent:

6929944, Aug 16, 2005

Filed:

Aug 31, 2001

Appl. No.:

09/945145

Inventors:

Robert S. Matson - Orange CA, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

C12M001/34
C12Q001/68
C12P019/34
A61K038/00
C07H001/00
C07H021/02
C07H021/04

US Classification:

4352872, 435 6, 435 71, 435 911, 4352871, 530300, 530350, 536 231, 536 243

Abstract:

The present invention is directed to the production of a sample microarray for use in detecting one or more target biopolymers in the sample. The sample microarray of this invention is formed by distributing equivalent amounts of a single sample at discrete, spatially defined locations on a substrate. Each site in the microarray, thus, has the same composition of target biopolymers. The microarray is then interrogated by one or more probes specific for one or more the target biopolymers.

US Patent:

6984485, Jan 10, 2006

Filed:

Apr 23, 2002

Appl. No.:

10/128350

Inventors:

Robert S. Matson - Orange CA, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

C12Q 1/68

US Classification:

435 4, 435 6, 435 71, 422104

Abstract:

Methods for preparing a substrate for the arraying and immobilizing of biomolecules or cells are described. The methods include providing a solid support and depositing by a chemical plasma-mediated polymerization process a polymeric coating on the surface. The polymeric coating comprises at least one pendant functional group capable of attaching a biomolecule or a cell. A material of the polymeric coating is preferably selected in such a way that its surface properties enhance binding between the pendant group and the biomolecule or cell. A substrate for the immobilization of biomolecules and cells is also provided. The substrate comprises a solid support made of a polymeric material. The solid support has at least one first pendant functional group suitable for attaching a first biomolecule or a first cell on its surface. The substrate further comprises a polymeric coating deposited on the surface of the substrate. The polymeric coating increases the attachment of the first biomolecule or the first cell to the first functional group.

US Patent:

7070740, Jul 4, 2006

Filed:

Sep 28, 2000

Appl. No.:

09/675020

Inventors:

Robert S. Matson - Orange CA, US
Daniel G. Morrow - Redwood City CA, US
Karen E. Lowe - Mill Creek WA, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

B01L 9/00

US Classification:

422104, 4352883, 4352885, 4353051, 422100, 422101, 422102, 422103, 366139, 366275, 436518

Abstract:

A microarray assay system using an array-of-arrays plate mounted on the top surface of a vacuum fixture, and an automatic handling system that handles the plate and fixture assembly. The Aplate includes a tray formed of a flexible material and a rigid frame for mounting on the fixture. The fixture has a plurality of orifices opening at the top surface and connected to a vacuum source to create a negative pressure to hold the flexible plate against the top surface of the fixture. The automatic handling systems that can be used with the plate and fixture assembly include a microarray printing machine, liquid handling robot, hybridization and incubation devices, and microarray imaging devices.

US Patent:

20010039018, Nov 8, 2001

Filed:

May 31, 2001

Appl. No.:

09/872052

Inventors:

Robert Matson - Orange CA, US
Raymond Milton - La Habra CA, US

Assignee:

Beckman Coulter, Inc.

International Classification:

C12Q001/68

US Classification:

435/006000

Abstract:

A method of attaching unmodified biopolymers, particularly, unmodified polynucleotides, directly to a solid support is provided. The method includes the steps of (a) providing unmodified biopolymers; (b) providing a solid support having at least one surface comprising pendant acyl fluoride functionalities; and (c) contacting the unmodified biopolymers with the solid support under a condition sufficient for allowing the attachment of the biopolymers to the solid support. The unmodified biopolymers may be nucleic acids, polypeptides, proteins, carbohydrates, lipids and analogues thereof The unmodified polynucleotides may be DNA, RNA or synthesized oligonucleotides. The DNA may be single or double stranded. A device including a solid support and unmodified biopolymers attached to the solid support by reaction with the pendant acyl fluoride functionalities of the solid support is also provided. The methods and devices of the present invention may be used in performing hybridization assays and immunoassays

US Patent:

20030198967, Oct 23, 2003

Filed:

Apr 23, 2002

Appl. No.:

10/128281

Inventors:

Robert Matson - Orange CA, US
Raymond Milton - La Habra CA, US
Robert Obremski - Yorba Linda CA, US
John Silzel - Yorba Linda CA, US

International Classification:

C12Q001/68
C12P019/60
C12M001/34
G01N033/543
B05D003/00

US Classification:

435/006000, 435/287200, 436/518000, 427/002110

Abstract:

Detection devices for multianalyte detection on a solid substrate, methods for the preparation of the devices and their use in analytical and diagnostic procedures are described. The detection devices include a solid substrate fabricated with an array of detection spots, the detection spots having an analyte sensor bound to the substrate by a universal binding ligand. The universal binding ligand is capable of binding multiple analyte sensors to create a multifunctional array. A process for producing the detection devices and assay methods employing microprinting technology are also described.

US Patent:

20040029156, Feb 12, 2004

Filed:

May 1, 2003

Appl. No.:

10/427658

Inventors:

Robert Matson - Orange CA, US
Jang Rampal - Yorba Linda CA, US

Assignee:

Beckman Coulter, Inc.

International Classification:

B05D003/00
C12Q001/68
C12M001/34

US Classification:

435/006000, 435/287200, 427/002110

Abstract:

An assay article for detection first biomolecules contained in a sample is described. The assay article includes a substrate having a modified surface and a first biomolecule directly adsorbed and immobilized on the modified surface of the substrate without linking moieties. A second biomolecule is bound to or adsorbed on the first biomolecule. Also disclosed is a method of making the assay article. A first biomolecule (other than an adhesive protein) is contacted with a modified surface of a substrate. The substrate is dried to directly adsorb the first biomolecule and immobilize it on the modified surface of the substrate without additional fixing steps to form an activated substrate. Then, a second biomolecule is contacted with the activated substrate under conditions sufficient for the first biomolecule to bind the second biomolecule.