Robert R Nagle

US Patent:

6337212, Jan 8, 2002

Filed:

Nov 2, 2000

Appl. No.:

09/705065

Inventors:

Robert Nagle - Mountain View CA
Robert S. Dubrow - San Carlos CA

Assignee:

Caliper Technologies Corp. - Mountain View CA

International Classification:

G01N 100

US Classification:

436174, 435 4, 435 6, 435 912, 204455

Abstract:

Reactor systems that include a reaction receptacle that includes a plurality of reservoirs disposed in the surface of a substrate. The reactor system also typically includes a temperature control element having at least a first heat exchanger thermally coupled to it. The heat exchanger is, in turn, disposed within the at least one of the reservoirs whereby the heat exchanger transfers heat to or from a fluid disposed within the reservoir, which heat is conducted to or from the temperature control element.

US Patent:

6498353, Dec 24, 2002

Filed:

Oct 24, 2001

Appl. No.:

10/038525

Inventors:

Robert Nagle - Mountain View CA
Colin B. Kennedy - Greenbrae CA

Assignee:

Caliper Technologies - Mountain View CA

International Classification:

G01N 1506

US Classification:

250573, 250216

Abstract:

The present invention provides microfluidic devices and systems that utilize optical detection systems, and where one or more light altering optical elements are integrated into the body structure of the microfluidic device. The resulting devices perform at least a portion of the optical manipulations used in the optical detection method employed.

US Patent:

6498497, Dec 24, 2002

Filed:

Aug 13, 1999

Appl. No.:

09/374878

Inventors:

Calvin Y. H. Chow - Portola Valley CA
Morten J. Jensen - San Francisco CA
Colin B. Kennedy - Mill Valley CA
Michael M. Lacy - Ben Lomond CA
Robert E. Nagle - Mountain View CA

Assignee:

Caliper Technologies Corp. - Mountain View CA

International Classification:

G01R 3500

US Classification:

324601, 324607

Abstract:

A microfluidic controller and detector system and method for performing screening assays are disclosed. The microfluidic controller and detector system comprises a fluidic chip that includes at least two intersecting channels and a detection zone, a fluid direction system comprising an electrical interface configured for electrical contact with the at least two intersecting channels, an optics block having an objective lens disposed adjacent the detection zone, and a control system coupled to the optics block and adapted to receive and analyze data from the optics block. The electrical interface generally includes electrodes configured for electrical contact with the intersecting channels and coupled to electrode channels for supplying electrical input to the electrodes. A reference channel is optionally provided to calibrate the electrode channels.

US Patent:

6541274, Apr 1, 2003

Filed:

Oct 17, 2001

Appl. No.:

09/981298

Inventors:

Robert Nagle - Mountain View CA
Robert S. Dubrow - San Carlos CA

Assignee:

Caliper Technologies Corp. - Mountain View CA

International Classification:

G01N 100

US Classification:

436180, 436177, 435 6, 435 912, 204451

Abstract:

Reactor systems that include a reaction receptacle that includes a plurality of reservoirs disposed in the surface of a substrate. The reactor system also typically includes a temperature control element having at least a first heat exchanger thermally coupled to it. The heat exchanger is, in turn, disposed within the at least one of the reservoirs whereby the heat exchanger transfers heat to or from a fluid disposed within the reservoir, which heat is conducted to or from the temperature control element.

US Patent:

6635487, Oct 21, 2003

Filed:

May 7, 2001

Appl. No.:

09/850855

Inventors:

Ernest C. W. Lee - Palo Alto CA
Robert Nagle - Mountain View CA
Richard J. McReynolds - San Jose CA
David Chazan - Palo Alto CA
Robert S. Dubrow - San Carlos CA

Assignee:

Caliper Technologies Corp. - Mountain View CA

International Classification:

G01N 3100

US Classification:

436 19, 436 8, 436164, 436172, 422 8205, 422 8208

Abstract:

A test device for use as a fluorescent standard in microfluidic analytical detection systems includes one or more slits that correspond to, and are of similar dimension to, one or more microchannels in a detection region on a corresponding analysis chip. A fluorescent material is attached to the test device on the side opposite the illumination source such that excitation radiation passes through the slit(s), which defines the focal plane of the illumination optics, and impinges on the fluorescent material thereby causing the fluorescent material to fluoresce. By displacing the fluorescent material relative to the focal plane, the intensity of the radiation exciting the fluorescent material is dispersed relative to the intensity of the radiation at the focal plane, and concomitantly the strength of the resulting fluorescent signal is reduced. An optional spacer is provided to increase the distance of the fluorescent material from the focal plane so as to increase the dispersion of the radiation (decrease the intensity impinging on the fluorescent material). The strength of the resulting fluorescent signal from the fluorescent material can be controlled by selecting a spacer with the appropriate depth.

US Patent:

6756019, Jun 29, 2004

Filed:

Apr 6, 2000

Appl. No.:

09/544711

Inventors:

Robert S. Dubrow - San Carlos CA
Colin B. Kennedy - Mill Valley CA
Robert Nagle - Mountain View CA
David Chazan - Palo Alto CA
Ernest C. W. Lee - Palo Alto CA
Khushroo Gandhi - Sunnyvale CA
Calvin Y. H. Chow - Portola Valley CA

Assignee:

Caliper Technologies Corp. - Mountain View CA

International Classification:

B01L 300

US Classification:

422102, 422 681, 422 99, 422100, 422103, 436174, 436179, 436180

Abstract:

The present invention provides microfluidic devices that comprise a body structure comprising at least a first microscale channel network disposed therein. The body structure has a plurality of ports disposed in the body structure, where each port is in fluid communication with one or more channels in the first channel network. The devices also include a cover layer comprising a plurality of apertures disposed through the cover layer. The cover layer is mated with the body structure whereby each of the apertures is aligned with a separate one of the plurality of ports. Rings are optionally disposed between the cover layer and the body structure and circumferentially around pairs of aligned apertures and ports. The devices also optionally include conductive coatings and membranes. The invention additionally provides methods of controlling the delivery of a composition of material into a microfluidic device.

US Patent:

6986837, Jan 17, 2006

Filed:

Aug 9, 2002

Appl. No.:

10/215885

Inventors:

Calvin Y. H. Chow - Portola Valley CA, US
Morten J. Jensen - San Francisco CA, US
Colin B. Kennedy - Mill Valley CA, US
Michael M. Lacy - Ben Lomond CA, US
Robert E. Nagle - Mountain View CA, US

Assignee:

Caliper Life Sciences, Inc. - Mountain View CA

International Classification:

G01N 27/453

US Classification:

204603, 422 99

Abstract:

A microfluidic controller and detector system and method for performing screening assays are disclosed. The microfluidic controller and detector system comprises a fluidic chip that includes at least two intersecting channels and a detection zone, a fluid direction system comprising an electrical interface configured for electrical contact with the at least two intersecting channels, an optics block having an objective lens disposed adjacent the detection zone, and a control system coupled to the optics block and adapted to receive and analyze data from the optics block. The electrical interface generally includes electrodes configured for electrical contact with the intersecting channels and coupled to electrode channels for supplying electrical input to the electrodes. A reference channel is optionally provided to calibrate the electrode channels.

US Patent:

20020009392, Jan 24, 2002

Filed:

Mar 26, 2001

Appl. No.:

09/818266

Inventors:

Jeffrey Wolk - Half Moon Bay CA, US
J. Parce - Palo Alto CA, US
Robert Nagle - Mountain View CA, US
Colin Kennedy - Greenbrae CA, US
Syed Husain - Fremont CA, US
Advit Bhatt - Union City CA, US
Garrett Unno - San Jose CA, US

International Classification:

B01L003/02
G01N001/14

US Classification:

422/063000, 436/180000, 422/067000, 422/100000, 422/104000, 204/453000, 204/604000

Abstract:

Methods for reducing fluid carryover by microfluidic devices including capillary element and/or fluid motion. Capillary elements coated with hydrophobic or hydrophilic coatings to resist fluid carryover are also provided. Microfluidic device handling systems are additionally included.