Robert C. Nutter - El Cerrito CA Lucy C. Panganiban - San Francisco CA
Assignee:
Stauffer Chemical Co. - Westport CT
International Classification:
C12N 100 C12N 1500
US Classification:
435317
Abstract:
Plasmids derived from Corynebacterium glutamicum, particularly pRN3. 1, of suitable size, about 3. 1 kilobases in length and weight about 2. times. 10. sup. 6 daltons, and having a limited number of restriction sites therein, suitable as vehicles for genetic engineering of Corynebacterium.
Patricia A. Okubara - Richmond CA Dorothy A. Pierce - Richmond CA Robert C. Nutter - Richmond CA
Assignee:
Stauffer Chemical Co. - Westport CT
International Classification:
C12N 100 C12N 1500
US Classification:
435317
Abstract:
Recombinant plasmids adopted for transformation of prokaryotic and eukaryotic hosts having an insertion site for at least one DNA fragment are provided. In a preferred embodiment, nucleotide sequences coding for methotrexate-resistant dehydrofolate reductase and a nucleotide sequence, a portion thereof coding for a promoter, are ligated into the insertion site.