Robert Allen Pope

US Patent:

7939331, May 10, 2011

Filed:

Mar 7, 2006

Appl. No.:

11/368996

Inventors:

John F. Leite - Vista CA, US
Robert Marshall Pope - San Marcos CA, US
Mahbod Hajivandi - Vista CA, US
Xiquan Liang - Escondido CA, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

G01N 30/72
C12P 1/00

US Classification:

436 86, 436173, 435 41

Abstract:

The invention provides methods for quantifying biomolecules, such as polypeptides in mass spectrometric analysis. The methods include use of a biomolecule standard having at least one atomic isotope different than that of the naturally occurring isotopes in the biomolecule of interest. Methods of the present invention also include methods for quantifying biomolecules where the copy biomolecule standard is made by expressing the biomolecule using a recombinant cell. Further included are the biomolecule standards themselves, method for making such standards, kits, systems, reagents, and engineered cells relating to the use of biomolecule standards in mass spectrometric analysis.

US Patent:

20060023808, Feb 2, 2006

Filed:

May 17, 2005

Appl. No.:

11/131744

Inventors:

Mahbod Hajivandi - Vista CA, US
Robert Pope - San Marcos CA, US
Mehrnoosh Sadeghi - Aliso Viejo CA, US
Xiquan Liang - Escondido CA, US
John Leite - Vista CA, US

International Classification:

H04L 25/49

US Classification:

375297000

Abstract:

The invention provides compositions, kits, and methods for calibrating a mass spectrometer using two or more recombinant proteins and one or more energy-absorbing molecules. The recombinant proteins of the invention display high purities, making them suitable for use in mass spectrometry.

US Patent:

20060214104, Sep 28, 2006

Filed:

Oct 26, 2005

Appl. No.:

11/258363

Inventors:

Robert Pope - San Marcos CA, US
John Leite - Vista CA, US
Mahbod Hajivandi - Vista CA, US
Charles Shevlin - San Diego CA, US
Timothy Updyke - Temecula CA, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

B01D 59/44
H01J 49/00

US Classification:

250297000

Abstract:

Compositions and methods for mass spectroscopy are disclosed. The compositions and methods relate to the analysis of proteins and other biopolymers using mass spectroscopy, particularly matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF MS).

US Patent:

20070105181, May 10, 2007

Filed:

May 4, 2006

Appl. No.:

11/417264

Inventors:

Robert Pope - San Marcos CA, US
Xiquan Liang - Escondido CA, US
Mahbod Hajivandi - Vista CA, US
John Leite - Vista CA, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12Q 1/37

US Classification:

435023000, 436086000

Abstract:

Methods are provided for identifying proteins that are differentially expressed in disease state and normal cells. Stable isotope labeling of cells in culture allows for the identification of a multiplicity of proteins whose differential abundance in normal and disease state cells can be indicative of the disease state. Biomarkers are identified for breast cancer, in which the biomarkers are proteins having a two-fold or greater difference in abundance between breast cancer and normal cells. Identified biomarkers can be used detection methods that can provide diagnosis, typing, staging, or prognosis of cancer, such as breast cancer, or can be used to predict the response of cancer, such as breast cancer, to one or more anti-cancer agents.

US Patent:

20090226913, Sep 10, 2009

Filed:

Dec 19, 2008

Appl. No.:

12/340341

Inventors:

Robert M. Pope - San Marcos CA, US
Xiquan Liang - Escondido CA, US
Mahbod Hajivandi - Vista CA, US
John Leite - Vista CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12Q 1/68
C12Q 1/00
G01N 33/574
G01N 33/00

US Classification:

435 6, 435 4, 435 723, 436 86, 436 94

Abstract:

Methods are provided for identifying proteins that are differentially expressed in disease state and normal cells. Stable isotope labeling of cells in culture allows for the identification of a multiplicity of proteins whose differential abundance in normal and disease state cells can be indicative of the disease state. Biomarkers are identified for breast cancer, in which the biomarkers are proteins having a two-fold or greater difference in abundance between breast cancer and normal cells. Identified biomarkers can be used detection methods that can provide diagnosis, typing, staging, or prognosis of cancer, such as breast cancer, or can be used to predict the response of cancer, such as breast cancer, to one or more anti-cancer agents.

US Patent:

20100187475, Jul 29, 2010

Filed:

Sep 2, 2009

Appl. No.:

12/553017

Inventors:

Robert Marshall Pope - San Marcos CA, US
John Leite - Vista CA, US
Mahbod Hajivandi - Vista CA, US
Charles George Shevlin - San Diego CA, US
Timothy Updyke - Temecula CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C09K 3/00

US Classification:

252184, 2524081

Abstract:

Compositions and methods for mass spectroscopy are disclosed. The compositions and methods relate to the analysis of proteins and other biopolymers using mass spectroscopy, particularly matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF MS).

US Patent:

20100207051, Aug 19, 2010

Filed:

Dec 19, 2007

Appl. No.:

12/519746

Inventors:

Geir Fonnum - Fjellhamar, NO
Torkel Stene - Oslo, NO
Nini Hofsloekken Kjus - Oslo, NO
Stine Louise Bergholtz - Oslo, NO
Kasper Engholm-Keller - Odense, DK
Finn Kirpekar - Odense, DK
Martin Larsen - Odense, DK
Robert Marshall Pope - San Marcos CA, US
Olen N. Jensen - Odense, DK
Liang Xiquan - Escondido CA, US

Assignee:

INVITROGEN DYNAL AS - Minneapolis MN
University of Southern Denmark - Odense M

International Classification:

H01F 1/00

US Classification:

252 6251R

Abstract:

Monodisperse polymer microparticles comprising polystyrene or polyacrylate, wherein said particles have a coating formed of at least one transition metal oxide or porous polymer microparticles comprising polystyrene or polyacrylate, wherein said particles have a coating formed of at least one transition metal oxide. The use of such particles in a method for isolation of phosphoproteins from a sample containing phosphoproteins or for isolating nucleic acid from a sample containing nucleic acid is also described

US Patent:

20110226942, Sep 22, 2011

Filed:

Jan 18, 2011

Appl. No.:

13/008879

Inventors:

MAHBOD R. HAJIVANDI - VISTA CA, US
ROBERT M. POPE - CORALVILLE IA, US
MEHRNOOSH SADEGHI - ALISO VIEJO CA, US
XIQUAN LIANG - ESCONDIDO CA, US
JOHN F. LEITE - VISTA CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - CARLSBAD CA

International Classification:

H01J 49/26
G01N 31/00
H01J 49/00

US Classification:

250282, 436 15, 2502521

Abstract:

The invention provides compositions, kits, and methods for calibrating a mass spectrometer using two or more recombinant proteins and one or more energy-absorbing molecules. The recombinant proteins of the invention display high purities, making them suitable for use in mass spectrometry.