Robert D Reis

US Patent:

6509454, Jan 21, 2003

Filed:

Apr 27, 2000

Appl. No.:

09/561734

Inventors:

Robert Van Reis - Redwood City CA

Assignee:

Genentech, Inc. - South San Francisco CA

International Classification:

A23J 100

US Classification:

530412, 530417, 530344, 5303871, 210650, 210652, 210767, 2105121, 436828, 436161, 435DIG 1, 435DIG 34

Abstract:

The present invention is directed to novel methods for enhancing the ability to separate a species of interest from other different species present in a free solution mixture thereof which takes advantage of interactions that occur between soluble, small molecular weight ligands and the species of interest. The small molecular weight ligands employed in the present invention function to interact with a species of interest in a mixture of different species through either affinity, hydrophobic and/or ionic interactions, thereby altering the molecular weight, hydrodynamic volume and/or isoelectric point of the species of interest and rendering it separable from other component(s) in the mixture.

US Patent:

7323553, Jan 29, 2008

Filed:

Apr 25, 2003

Appl. No.:

10/423299

Inventors:

Robert Fahrner - San Mateo CA, US
Deborah Follman - West Lafayette IN, US
Benedicte Lebreton - San Francisco CA, US
Robert van Reis - Emerald Hills CA, US

Assignee:

Genentech, Inc. - South San Francisco CA

International Classification:

A23J 1/00

US Classification:

530412

Abstract:

The present invention relates to a method for protein purification that involves the combination of non-affinity chromatography with HPTFF.

US Patent:

20020108907, Aug 15, 2002

Filed:

Apr 12, 2002

Appl. No.:

10/121360

Inventors:

Robert Van Reis - Redwood City CA, US

International Classification:

B01D011/00

US Classification:

210/637000, 210/644000, 210/137000, 210/321650, 210/321720, 210/511000

Abstract:

The present invention includes a system and method for transferring a selected solute species from a fluid mixture to a fluid media. The system of the present invention includes a convergent channel for passing a fluid mixture containing a selected species tangentially across the first surface of a porous membrane. A fluid media is directed to flow tangentially over the second surface of the membrane. As the fluid mixture and the fluid media flow on opposite sides of the membrane, the selected species traverses the membrane leaving the fluid mixture and entering the fluid media. The volumetric loss to the fluid mixture associated with the loss of the selected species is compensated for by the convergency of the channel. As a result a constant velocity is maintained over the membrane, maximizing the selectivity of the filtration process.

US Patent:

20030121854, Jul 3, 2003

Filed:

Nov 18, 2002

Appl. No.:

10/299341

Inventors:

Robert Reis - Redwood City CA, US

International Classification:

B01D011/00

US Classification:

210/634000, 210/638000, 210/639000, 210/656000, 210/644000, 210/650000, 435/004000, 435/006000

Abstract:

The present invention is directed to novel methods for enhancing the ability to separate a species of interest from other different species present in a free solution mixture thereof which takes advantage of interactions that occur between soluble, small molecular weight ligands and the species of interest. The small molecular weight ligands employed in the present invention function to interact with a species of interest in a mixture of different species through either affinity, hydrophobic and/or ionic interactions, thereby altering the molecular weight, hydrodynamic volume and/or isoelectric point of the species of interest and rendering it separable from other component(s) in the mixture.

US Patent:

20030178367, Sep 25, 2003

Filed:

Apr 22, 2003

Appl. No.:

10/421177

Inventors:

Robert van Reis - Redwood City CA, US

International Classification:

B01D061/00

US Classification:

210/637000, 210/137000, 210/195200, 210/650000, 210/321840, 210/641000

Abstract:

The present invention includes a system and method for transferring a selected solute species from a fluid mixture to a fluid media. The system of the present invention includes a convergent channel for passing a fluid mixture containing a selected species tangentially across the first surface of a porous membrane. A fluid media is directed to flow tangentially over the second surface of the membrane. As the fluid mixture and the fluid media flow on opposite sides of the membrane, the selected species traverses the membrane leaving the fluid mixture and entering the fluid media. The volumetric loss to the fluid mixture associated with the loss of the selected species is compensated for by the convergency of the channel. As a result a constant velocity is maintained over the membrane, maximizing the selectivity of the filtration process.

US Patent:

20030178368, Sep 25, 2003

Filed:

Apr 16, 2003

Appl. No.:

10/417715

Inventors:

Robert van Reis - Redwood City CA, US

International Classification:

B01D061/00
B01D071/10

US Classification:

210/649000, 210/500290

Abstract:

The invention relates to charged filtration membranes and their use for separation of a protein from solvent, low molecular weight solutes or a mixture of proteins. Modification of the membranes to generate charge includes modification of membrane pores to alter charge within a pore and alter the size of a pore. Consequently, the protein is separated from other solutes in a mixture based on size as well as net protein charge and membrane charge.

US Patent:

20060157412, Jul 20, 2006

Filed:

Sep 29, 2005

Appl. No.:

11/241279

Inventors:

Robert van Reis - Redwood City CA, US

International Classification:

B01D 61/00

US Classification:

210644000, 210500290, 210506000, 210508000, 264041000, 435006000, 435007100, 210651000

Abstract:

The invention relates to charged filtration membranes and their use for separation of a protein from solvent, low molecular weight solutes or a mixture of proteins. Modification of the membranes to generate charge includes modification of membrane pores to alter charge within a pore and alter the size of a pore. Consequently, the protein is separated from other solutes in a mixture based on size as well as net protein charge and membrane charge.

US Patent:

20060234226, Oct 19, 2006

Filed:

Apr 25, 2003

Appl. No.:

10/512233

Inventors:

Robert Fahner - San Mateo CA, US
Deborah Follman - West Lafayette IN, US
Benedicte Lebreton - San Francisco CA, US
Robert Van Reis - Emerald Hills CA, US

International Classification:

C07K 14/775
C07K 14/705
C07K 14/56
C07K 14/54
C07K 14/52

US Classification:

435006000, 530388100, 530350000, 530359000, 530399000, 530303000, 530414000, 530351000

Abstract:

The present invention relates to a method for protein purification that involves the combination of non-affinity chromatography with HPTFF.