Robert E Shell

US Patent:

20130029377, Jan 31, 2013

Filed:

Jul 25, 2012

Appl. No.:

13/557855

Inventors:

Maire H. CAPARON - Chesterfield MO, US
Kevin J. RUST - Manchester MO, US
Alan K. HUNTER - Damascus MD, US
Joseph K. McLAUGHLIN - Ballwin MO, US
Kristin E. THOMAS - Wildwood MO, US
John T. HERBERG - Portage MI, US
Robert E. SHELL - Charlotte NC, US
Paul B. LANTER - Edwardsville IL, US
Bruce F. BISHOP - Warrenton MO, US
Robert L. DUFIELD - Windham NH, US
Xing WANG - Wildwood MO, US
Sa V. HO - Boston MA, US

Assignee:

PFIZER INC. - Groton CT

International Classification:

C12N 1/21
C12P 21/00

US Classification:

435 691, 43525233

Abstract:

Apolipoprotein A-1 Milano (ApoA-1M), the protein component of a high-density lipoprotein (HDL) mimic with promising potential for reduction of atherosclerotic plaque, is produced on a large scale by expression in . Significant difficulty with clearance of host cell proteins (HCPs) was experienced in the original manufacturing process, despite lengthy purification. Analysis of purified protein solutions and intermediate process samples led to the identification of several major HCPs, and a bacterial protease causing the production of a truncated species of ApoA-1M co-purifying with the product. Deletions in these genes from the original host strain succeeded in substantially reducing the levels of HCPs without adversely affecting overall fermentation productivity, contributing to a much more efficient and robust new manufacturing process.