Robert A Slone

US Patent:

20220282282, Sep 8, 2022

Filed:

Feb 28, 2022

Appl. No.:

17/682793

Inventors:

- Germantown MD, US
Robert Daniel SLONE - Germantown MD, US
Steven ROBERTS - Germantown MD, US
Chad B. GREEN - Campbell CA, US
Vincent SO - Campbell CA, US

International Classification:

C12N 15/87
A61K 9/08
A61K 47/26
A61K 47/20
A61K 47/10
A61K 47/36

Abstract:

An electroporation buffer comprising: a solvent; a sugar; a chloride salt; and a buffering agent. In certain embodiments: the solvent is water; the sugar is glucose or mannitol; the chloride salt is potassium chloride (KCl) or magnesium chloride (MgCl); and the buffering agent is sodium phosphate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and/or dimethyl sulfoxide (DMSO). A method of electroporation, the method comprising applying an electric current to a suspension comprising: isolated eukaryotic cells; a biological material that is exogenous to the cells; and the aforementioned buffer. A recombinant cell produced using such a method. An electroporation apparatus comprising: one or more chambers; one or more pairs of electrodes configured to generate electric fields within the one or more chambers, wherein each electric field corresponds to one chamber; and a flow channel. A method for electroporation comprising utilizing the aforementioned electroporation apparatus.