Robert K Swenson

US Patent:

44394225, Mar 27, 1984

Filed:

Feb 2, 1982

Appl. No.:

6/345054

Inventors:

Robert M. Swenson - Philadelphia PA
Gerald D. Shockman - Philadelphia PA
Toby K. Eisenstein - Wyndmoor PA
Roberta B. Carey - Princeton Junction NJ

Assignee:

Research Corporation - New York NY

International Classification:

A61K 3902
C08B 3700
C12P 1904

US Classification:

424 92

Abstract:

Pure Group B Streptococcus antigen and Group B, Type III Streptococcus antigen are isolated, separated and purified from the culture medium in which the Group B, Type III Streptococcus are grown. The process involves (a) culturing the Group B, Type III Streptococcus in a nutrient medium, free of animal proteins and lipids, containing from 1 to 5% by weight glucose and from 0. 04 M to 0. 16 M phosphates; (b) separating the bacterial cells from the culture medium; (c) extracting and separating the Group B antigens and the Type III antigens from the culture medium supernatant by means of an anion exchanger; and, (d) purifying the resulting antigenic materials by chromatography on an agarose gel column. The antigens can be used as vaccines against infections caused by Group B or Group B, Type III Streptococcus.