Robert M Worden

US Patent:

8435773, May 7, 2013

Filed:

May 10, 2006

Appl. No.:

11/914340

Inventors:

Robert M. Worden - Holt MI, US
Robert Y. Ofoli - Okemos MI, US
Brian L. Hassler - Lake Orion MI, US
Neeraj Kohli - East Lansing MI, US
Ilsoon Lee - Okemos MI, US

Assignee:

Board of Trustees of Michigan State University - East Lansing MI

International Classification:

C12N 11/00
C12N 11/16
C12Q 1/34
C12Q 1/00
C12Q 1/26

US Classification:

435174, 435 18, 435 25, 4352831, 4352871, 20440314

Abstract:

A chemical composite useful for preparing a bioelectronic device includes a biologically active compound, such as an enzyme, that is bound directly or indirectly to a polyelectrolyte, which can be reversibly coupled to a chemically treated electrically conductive substrate by electrostatic forces to provide biomimetic sensors, catalyst systems, and other devices having an electrode that can be regenerated and reused. Required or desired cofactors, mediators or the like may be incorporated into the devices, typically by bonding them to the treated substrate and/or the polyelectrolyte.

US Patent:

8623196, Jan 7, 2014

Filed:

May 15, 2008

Appl. No.:

12/121389

Inventors:

Neeraj Kohli - Cambridge MA, US
Devesh Srivastava - East Lansing MI, US
Rudy J. Richardson - Ann Arbor MI, US
Jun Sun - Hockessin DE, US
Ilsoon Lee - Okemos MI, US
Robert M. Worden - Holt MI, US

Assignee:

Michigan State University - East Lansing MI
The Regents of the University of Michigan - Ann Arbor MI

International Classification:

G01N 27/327

US Classification:

2057775, 20440304, 20440314

Abstract:

The present invention provides compositions, devices and methods for detecting esterase activity. The present invention also provides devices and methods of detecting esterase inhibitors, for example, organophosphates. In particular, the present invention provides a biosensor comprising Neuropathy Target Esterase (NTE) polypeptides. Further, the present invention relates to medicine, industrial chemistry, agriculture, and homeland security.

US Patent:

20100314248, Dec 16, 2010

Filed:

Apr 23, 2010

Appl. No.:

12/766169

Inventors:

Robert M. Worden - Holt MI, US
Brian L. Hassler - Lake Orion MI, US
Lawrence T. Drzal - Okemos MI, US
Ilsoon Lee - Okemos MI, US

International Classification:

C25B 11/06
C25B 11/12
G01N 27/327
B05D 1/04
C25B 9/00

US Classification:

20440314, 20429011, 204400, 20429007, 427458, 204252, 977734, 977742

Abstract:

An inexpensive, easily renewable bioelectronic device useful for bioreactors, biosensors, and biofuel cells includes an electrically conductive carbon electrode and a bioelectronic interface bonded to a surface of the electrically conductive carbon electrode, wherein the bioelectronic interface includes catalytically active material that is electrostatically bound directly or indirectly to the electrically conductive carbon electrode to facilitate easy removal upon a change in pH, thereby allowing easy regeneration of the bioelectronic interface.

US Patent:

60227273, Feb 8, 2000

Filed:

Jan 12, 1998

Appl. No.:

9/005939

Inventors:

Robert Mark Worden - Holt MI
Alec B. Scranton - Boulder CO

Assignee:

Board of Trustees Operating Michigan State University - East Lansing MI

International Classification:

B01D 5362
B01J 1300
C12N 134

US Classification:

435243

Abstract:

A method for forming gas or liquid aphrons with reversible surfactants or emulsifiers is described. Polymers which change their emulsifying properties upon change of pH, temperature or other condition are used to form the aphrons. The aphrons are useful for chemical reactions and separations, mass transfer processes, and for biological processes.

US Patent:

63262037, Dec 4, 2001

Filed:

Dec 14, 1999

Appl. No.:

9/460591

Inventors:

Robert Mark Worden - Holt MI
Tyler T. Ames - Newark DE

Assignee:

Board of Trustees operating Michigan State University - East Lansing MI

International Classification:

C12N 502
C12N 120
C12N 1300

US Classification:

435420

Abstract:

A method and bioreactor (10) apparatus is described for maintaining cells in a culture medium containing gas or liquid aphrons. A preferred microbubble generator (42, 42A) of a gas which is necessary for maintaining the cells to produce plant described chemicals is described. Bioparticles (11) containing magnetically susceptible particles are provided in a column (12) surrounded by solenoids (13, 14, 14A) which act to hold the bioparticles in position in the column. Preferably, one of the solenoids with a magnetically susceptible screen (24) acts as a valve to allow a portion of the bioparticles to be removed from the column.

US Patent:

60543191, Apr 25, 2000

Filed:

Feb 3, 1998

Appl. No.:

9/018327

Inventors:

Robert Mark Worden - Holt MI
Tyler T. Ames - Newark DE

Assignee:

Board of Trustees operating Michigan State University - East Lansing MI

International Classification:

C12N 502
C12N 120
C12N 1300

US Classification:

435420

Abstract:

A method and bioreactor (10) apparatus is described for maintaining cells in a culture medium containing gas or liquid aphrons. A preferred microbubble generator (42, 42A) of a gas which is necessary for maintaining the cells to produce plant derived chemicals is described. Bioparticles (11) containing magnetically susceptible particles are provided in a column (12) surrounded by a solenoids (13, 14, 14A) which act to hold the bioparticles in position in the column. Preferably, one of the solenoids with a magnetically susceptible screen (24) acts as a valve to allow a portion of the bioparticles to be removed from the column.

US Patent:

58468293, Dec 8, 1998

Filed:

Nov 8, 1996

Appl. No.:

8/748508

Inventors:

Robert Mark Worden - Holt MI
Kenneth C. Sink - Okemos MI
Tyler T. Ames - Lansing MI
Vicki S. Thompson - Idaho Falls ID

Assignee:

Board of Trustees operating Michigan State University - East Lansing MI

International Classification:

C12N 502
C12N 504

US Classification:

435420

Abstract:

A method and bioreactor (10) apparatus is described for growing plant cells, particularly to produce plant derived chemicals is described. Bioparticles (11) containing magnetically susceptible particles are provided in a column (12) surrounded by a solenoids (13, 14, 14A) which act to hold the bioparticles in position in the column. One of the solenoids with a magnetically susceptible screen (24) acts as a valve to allow a portion of the bioparticles to be removed from the column. The method is particularly described for producing daidzein and genistein.

US Patent:

6087158, Jul 11, 2000

Filed:

Apr 27, 1999

Appl. No.:

9/302006

Inventors:

Robert Mark Worden - Holt MI
Tyler T. Ames - Newark DE

Assignee:

Board of Trustees Operating Michigan State University - East Lansing MI

International Classification:

C12M 300

US Classification:

4352961

Abstract:

A method and bioreactor (10) apparatus is described for maintaining cells in a culture medium containing gas or liquid aphrons. A preferred microbubble generator (42, 42A) of a gas which is necessary for maintaining the cells to produce plant derived chemicals is described. Bioparticles (11) containing magnetically susceptible particles are provided in a column (12) surrounded by a solenoids (13, 14, 14A) which act to hold the bioparticles in position in the column. Preferably, one of the solenoids with a magnetically susceptible screen (24) acts as a valve to allow a portion of the bioparticles to be removed from the column.