Roberta Jean Jackson

US Patent:

6599738, Jul 29, 2003

Filed:

Aug 2, 2001

Appl. No.:

09/919901

Inventors:

Karen Elizabeth Potts - Solana Beach CA
Roberta Lynn Jackson - San Diego CA
Amy Karen Patick - Escondido CA

Assignee:

Agouron Pharmaceuticals, Inc. - San Diego CA

International Classification:

C12N 1574

US Classification:

4353201, 435 5, 536 232, 536 234, 536 2372

Abstract:

A cell-based assay system in which the detection of the reporter gene activity, or secreted alkaline phosphatase (SEAP), is dependent upon the protease activity of the Hepatitis C virus NS3 gene product. This system can be used to assess the activity of candidate protease inhibitors in a mammalian cell-based assay system. The assay system is simpler than previously described assays due to the use of SEAP which allows the reporter gene activity to be quantified by measuring the amount of secreted gene product in the cell media by monitoring the conversion of luminescent or colorimetric alkaline phosphatase substrate.

US Patent:

6790612, Sep 14, 2004

Filed:

Jul 10, 2002

Appl. No.:

10/191966

Inventors:

Karen Elizabeth Potts - Solana Beach CA
Roberta Lynn Jackson - San Diego CA
Amy Karen Patick - Escondido CA

Assignee:

Agouron Pharmaceuticals, Inc. - San Diego CA

International Classification:

C12Q 170

US Classification:

435 5, 435325, 435367, 435358, 435364, 435352, 435 23, 435455

Abstract:

A cell-based assay system in which the detection of the reporter gene activity, or secreted alkaline phosphatase (SEAP), is dependent upon the protease activity of the Hepatitis C virus NS3 gene product. This system can be used to assess the activity of candidate protease inhibitors in a mammalian cell-based assay system. The assay system is simpler than previously described assays due to the use of SEAP which allows the reporter gene activity to be quantified by measuring the amount of secreted gene product in the cell media by monitoring the conversion of luminescent or calorimetric alkaline phosphatase substrate.

US Patent:

62809407, Aug 28, 2001

Filed:

Mar 8, 1999

Appl. No.:

9/263933

Inventors:

Karen Elizabeth Potts - Solana Beach CA
Roberta Lynn Jackson - San Diego CA
Amy Karen Patick - Escondido CA

Assignee:

Agouron Pharmaceuticals, Inc. - La Jolla CA

International Classification:

C12Q 168
C12Q 170

US Classification:

435 6

Abstract:

A cell-based assay system in which the detection of the reporter gene activity, or secreted alkaline phosphatase (SEAP), is dependent upon the protease activity of the Hepatitis C virus NS3 gene product. This system can be used to assess the activity of candidate protease inhibitors in a mammalian cell-based assay system. The assay system is simpler than previously described assays due to the use of SEAP which allows the reporter gene activity to be quantified by measuring the amount of secreted gene product in the cell media by monitoring the conversion of luminescent or calorimetric alkaline phosphatase substrate.