Robin L Butler

US Patent:

20040133931, Jul 8, 2004

Filed:

Jan 8, 2003

Appl. No.:

10/338546

Inventors:

William Gavin - Dudley MA, US
David Melican - Fiskdale MA, US
Robin Butler - Spencer MA, US

International Classification:

A01K067/00

US Classification:

800/008000

Abstract:

The present invention provides data to demonstrate that the re-fusion, of a mammalian karyoplast to an enucleated in vivo ovulated oocyte, following an unsuccessful initial simultaneous electrical fusion and activation event offers an additional alternative and improvement in the creation of activated and fused nuclear transfer-capable embryos for the production of live offspring in various mammalian non-human species including goats, pigs, rodents, primates, rabbits and cattle. Additionally, multiple electrical pulses offers an alternative and more efficient activation method in a simultaneous fusion and activation methodology for viable offspring production in a animal nuclear transfer program.

US Patent:

20050177882, Aug 11, 2005

Filed:

Jan 8, 2003

Appl. No.:

10/501305

Inventors:

William Gavin - Dudley MA, US
David Melican - Fiskdale MA, US
Robin Butler - Spencer MA, US

International Classification:

A01K067/027

US Classification:

800014000, 800015000, 800016000, 800017000

Abstract:

The present invention provides data to demonstrate that the re-fusion, of a mammalian karyoplast to an enucleated in vivo ovulated cocyte, following an unsuccessful initial simultaneous electrical fusion and activation event offers an additional alternative and improvement in the creation of activated and fused nuclear transfer-capable embryos for the production of live off-spring in various mammalian non-human species including goats, pigs, rodents, primates, rabbits and cattle. Additionally, multiple electrical pulses offers an alternative and more efficient activation method in a simultaneous fusion and activation methodology for viable offspring production in a animal nuclear transfer program.

US Patent:

20060174359, Aug 3, 2006

Filed:

Mar 28, 2006

Appl. No.:

11/391148

Inventors:

David Melican - Fiskdale MA, US
Robin Butler - Spencer MA, US
William Gavin - Dudley MA, US

International Classification:

A01K 67/027

US Classification:

800014000, 800021000

Abstract:

The present invention provides data to demonstrate that the fusion performance of a cell-line in procedures involving fusion and cleavage indices either alone or in combination are a means for selecting a cell lines that will be successful in a nuclear transfer or microinjection program. This technique and method of selecting a cell line offers an additional alternative and improvement in the creation of activated and fused nuclear transfer-capable embryos for the production of live offspring in various mammalian non-human species including goats, pigs, rodents, primates, rabbits and cattle.

US Patent:

20060191029, Aug 24, 2006

Filed:

Apr 20, 2006

Appl. No.:

11/408116

Inventors:

William Gavin - Dudley MA, US
David Melican - Fiskdale MA, US
Robin Butler - Spencer MA, US

International Classification:

A01K 67/027

US Classification:

800014000, 800015000, 800016000, 800017000, 800021000

Abstract:

The present invention provides data to demonstrate that the re-fusion, of a mammalian karyoplast to an enucleated in vivo ovulated oocyte, following an unsuccessful initial simultaneous electrical fusion and activation event offers an additional alternative and improvement in the creation of activated and fused nuclear transfer-capable embryos for the production of live offspring in various mammalian non-human species including goats, pigs, rodents, primates, rabbits and cattle. Additionally, multiple electrical pulses offers an alternative and more efficient activation method in a simultaneous fusion and activation methodology for viable offspring production in a animal nuclear transfer program.

US Patent:

20030204860, Oct 30, 2003

Filed:

Mar 25, 2003

Appl. No.:

10/396890

Inventors:

David Melican - Fiskdale MA, US
Robin Butler - Spencer MA, US
William Gavin - Dudley MA, US

International Classification:

A01K067/027

US Classification:

800/014000, 800/015000, 800/016000, 800/017000

Abstract:

The present invention provides data to demonstrate that the fusion performance of a cell-line in procedures involving fusion and cleavage indices either alone or in combination are a means for selecting a cell lines that will be successful in a nuclear transfer or microinjection program. This technique and method of selecting a cell line offers an additional alternative and improvement in the creation of activated and fused nuclear transfer-capable embryos for the production of live offspring in various mammalian non-human species including goats, pigs, rodents, primates, rabbits and cattle.