Rodolfo Arroyo Rodriguez

US Patent:

20090011451, Jan 8, 2009

Filed:

May 13, 2005

Appl. No.:

11/568832

Inventors:

Rodolfo R. Rodriguez - Cary NC, US
Douglas J. Darr - Timberlake NC, US

International Classification:

C12Q 1/02
G01N 21/00
G01N 21/64

US Classification:

435 29, 422 8205, 422 8208

Abstract:

The present invention relates to an leucocyte antigen mediated microfluidic assay and a microfluidic device for analyzing a subjects' body fluids containing leucocytes to determine if the subject has been previously exposed to a predetermined antigen.

US Patent:

20090233329, Sep 17, 2009

Filed:

Mar 26, 2007

Appl. No.:

12/294037

Inventors:

Rodolfo R. Rodriguez - Durham NC, US
Charles F. Galanaugh - West Milford NJ, US

International Classification:

C12Q 1/06
C12M 1/34

US Classification:

435 39, 4352871

Abstract:

The present invention relates to a device and method for the detection of mastitis or other disease from a body fluid of a mammal for example from cow's milk. The device and method relates to a wedge microfluidic chamber for using a minimal amount of fluid and being able to use the device to observe leukocytes in a mono-layer for the purpose of disease detection, cell counts or the like.

US Patent:

20140009596, Jan 9, 2014

Filed:

Jun 27, 2013

Appl. No.:

13/928741

Inventors:

David A. Calderwood - Chapel Hill NC, US
Tobias M. Heineck - Durham NC, US
David Newcomb - Morrisville NC, US
Chris Paul - Hillsborough NC, US
Jasper N. Pollard - Durham NC, US
Rodolfo R. Rodriguez - Cary NC, US
Demetris Young - Durham NC, US

International Classification:

G06T 7/00
G06F 1/20
G02B 21/26
G02B 21/00

US Classification:

348 79, 359368, 36167954, 359392

Abstract:

An automated microscope apparatus, comprises an outer housing having an external wall; optionally but preferably an internal wall in said housing, and configured to form a first compartment and a separate second compartment in said outer housing; a microscope assembly in said housing, preferably in said first compartment; and a microprocessor in said housing, preferably in said second compartment; and optionally but preferably a heat sink mounted on said housing external wall, preferably adjacent said second compartment, with said microprocessor thermally coupled to said heat sink and operatively associated with said microscope assembly.

US Patent:

20230096475, Mar 30, 2023

Filed:

Mar 12, 2021

Appl. No.:

17/905990

Inventors:

- Morrisville NC, US
Brendan Smith - Cary NC, US
Tobias M. Heineck - Durham NC, US
Stefano Bresolin - Garner NC, US
Rodolfo R. Rodriguez - Cary NC, US
Danielle Stewart Noel - Raleigh NC, US
Brian Pike - Durham NC, US
Oleg Kakhovsky - Raleigh NC, US
Jasper N. Pollard - Durham NC, US

International Classification:

G01N 33/50
G01N 33/49
G01N 21/64
G01N 15/05
G01N 15/14

Abstract:

A method of analyzing a blood sample from a subject includes loading the blood sample into a single chamber; acquiring, via an imaging system, a stack of serial focal plane images of the blood sample from a plurality of fields of view of the chamber; creating a virtual three dimensional image of the blood sample from selected ones of the stacks of serial focal plane images; and analyzing the virtual three dimensional image to identify blood formed elements within the blood sample. Identifying blood formed elements within the blood sample may include identifying a type and amount of white blood cells, and/or identifying an amount of red blood cells and/or hematocrit, and/or identifying an amount of platelets. Identifying blood formed elements within the blood sample may include determining numbers and percentage by volume in the blood sample of one or more types of white blood cells.

US Patent:

20210270803, Sep 2, 2021

Filed:

Feb 12, 2021

Appl. No.:

17/175114

Inventors:

- Morrisville NC, US
Mitchell Hockett - Raleigh NC, US
Stefano Bresolin - Garner NC, US
Erik J. Jensen - Hillsborough NC, US
Tobias M. Heineck - Durham NC, US
Jasper N. Pollard - Durham NC, US
Deborah Asion - Durham NC, US
Rodolfo R. Rodriguez - Cary NC, US
David A. Calderwood - Chapel Hill NC, US
Norah Gerow Bate - Raleigh NC, US
John Richard Sink - Raleigh NC, US
Jorge Carlos Correa - Raleigh NC, US

International Classification:

G01N 33/49

Abstract:

The present invention provides a method of identifying an animal for which antibiotic treatment, anti-infective treatment, probiotic treatment, immunostimulant treatment, additional monitoring and/or a separate or alternative management strategy is appropriate, comprising: a) collecting a sample from said animal; b) performing a leukocyte differential cell count on said sample; c) comparing said leukocyte differential cell count of (b) with an index of infection; and d) initiating antibiotic treatment, anti-infective treatment, probiotic treatment, immunostimulant treatment, additional monitoring and/or a separate or alternative management strategy of said animal on the basis of said comparing step.

US Patent:

20180172676, Jun 21, 2018

Filed:

Nov 13, 2017

Appl. No.:

15/810534

Inventors:

- Morrisville NC, US
David A. Calderwood - Chapel Hill NC, US
Tobias M. Heineck - Durham NC, US
David Newcomb - Morrisville NC, US
Chris Paul - Hillsborough NC, US
Jaspar N. Pollard - Durham NC, US
Rodolfo R. Rodriguez - Cary NC, US
Demetris Young - Durham NC, US

International Classification:

G01N 33/50
G02B 21/00
G06T 7/00
G02B 21/26
G06F 1/20

Abstract:

An automated microscope apparatus, comprises an outer housing having an external wall; optionally but preferably an internal wall in said housing, and configured to form a first compartment and a separate second compartment in said outer housing; a microscope assembly in said housing, preferably in said first compartment; and a microprocessor in said housing, preferably in said second compartment; and optionally but preferably a heat sink mounted on said housing external wall, preferably adjacent said second compartment, with said microprocessor thermally coupled to said heat sink and operatively associated with said microscope assembly.

US Patent:

20170276663, Sep 28, 2017

Filed:

Mar 22, 2017

Appl. No.:

15/466814

Inventors:

- Morrisville NC, US
Mitchell Hockett - Raleigh NC, US
Stefano Bresolin - Gamer NC, US
Erik J. Jensen - Hillsborough NC, US
Tobias M. Heineck - Durham NC, US
Jasper N. Pollard - Durham NC, US
Deborah Asion - Durham NC, US
Rodolfo R. Rodriguez - Cary NC, US
David A. Calderwood - Chapel Hill NC, US
Norah Gerow Bate - Raleigh NC, US
John Richard Sink - Raleigh NC, US
Jorge Carlos Correa - Raleigh NC, US

International Classification:

G01N 33/49

Abstract:

The present invention provides a method of identifying an animal for which antibiotic treatment, anti-infective treatment, probiotic treatment, immunostimulant treatment, additional monitoring and/or a separate or alternative management strategy is appropriate, comprising: a) collecting a sample from said animal; b) performing a leukocyte differential cell count on said sample; c) comparing said leukocyte differential cell count of (b) with an index of infection; and d) initiating antibiotic treatment, anti-infective treatment, probiotic treatment, immunostimulant treatment, additional monitoring and/or a separate or alternative management strategy of said animal on the basis of said comparing step.

US Patent:

20150072880, Mar 12, 2015

Filed:

May 9, 2013

Appl. No.:

14/239660

Inventors:

David A. Calderwood - Chapel Hill NC, US
Rodolfo R. Rodriguez - Cary NC, US

International Classification:

G01N 33/543
G01N 33/569

US Classification:

506 9, 422 69, 506 18, 4352872, 436501, 435 732, 435 733, 435 5, 435 737, 435 735, 435 734, 435 731, 435 736

Abstract:

A device for detecting at least one analyte generally comprises (i) a support having a chamber for receiving a biological fluid therein, wherein said chamber is an elongate chamber having a length axis; (ii) a carrier or agitator in said elongate chamber, said carrier or agitator having opposite end portions and a side portion, the carrier or agitator dimensioned to travel in said chamber along said length axis and/or permit said liquid sample to flow in the chamber therearound, either (or both) thereby agitating the liquid sample; and (iii) at least one anti-analyte antibody coupled to either the carrier and/or the chamber side wall. Methods of using the device are also described.