Roger Michael Hendry

US Patent:

8287878, Oct 16, 2012

Filed:

Apr 5, 2005

Appl. No.:

11/547236

Inventors:

David Kirkwood Clarke - Chester NY, US
Roger Michael Hendry - Warwick NY, US
Stephen A. Udem - New York NY, US
Christopher Lee Parks - Boonton NJ, US

Assignee:

Wyeth LLC - Madison NJ

International Classification:

A61K 39/12
C12N 7/01

US Classification:

4241991, 4352351, 4353201

Abstract:

The present invention broadly relates to the synergistic attenuation of vesicular stomatitis virus (VSV). More particularly, the invention relates to the identification of combined mutation classes which synergistically attenuate the pathogenicity of VSV vectors in mammals and immunogenic compositions thereof.

US Patent:

8647638, Feb 11, 2014

Filed:

Aug 31, 2012

Appl. No.:

13/600289

Inventors:

David Kirkwood Clarke - Chester NY, US
Roger Michael Hendry - Atlanta GA, US
Stephen A. Udem - New York NY, US
Christopher Lee Parks - Boonton NJ, US

Assignee:

Wyeth LLC - Madison NJ

International Classification:

A61K 39/205
A61K 39/12
C12N 7/01
C12N 15/63

US Classification:

4242241, 4241991, 4352351, 4353201

Abstract:

The present invention broadly relates to the synergistic attenuation of vesicular stomatitis virus (VSV), More particularly, the invention relates to the identification of combined mutation classes which synergistically attenuate the pathogenicity of VSV vectors in mammals and immunogenic compositions thereof.

US Patent:

20090162321, Jun 25, 2009

Filed:

Dec 16, 2008

Appl. No.:

12/335778

Inventors:

Christopher L. Parks - Boonton NJ, US
Susan E. Witko - New City NY, US
Maninder K. Sidhu - New City NY, US
J. Erik Johnson - Verona NJ, US
Roger Michael Hendry - Atlanta GA, US

Assignee:

Wyeth - Madison NJ

International Classification:

A61K 35/76
C12N 7/02
C07H 21/04
A61P 37/00

US Classification:

424 932, 435239, 536 2372

Abstract:

A method of producing propagation-defective Vesicular Stomatitis Virus (VSV) is provided. The method involves providing a cell that includes an optimized VSV G gene, wherein expression of VSV G protein from the optimized VSV G gene is inducible; and inducing the cell to express VSV G protein from the optimized VSV G gene. The method also involves infecting the induced cell with an attenuated VSV; growing the infected cells in culture; and recovering attenuated VSV from the culture.

US Patent:

20090175900, Jul 9, 2009

Filed:

Dec 16, 2008

Appl. No.:

12/335763

Inventors:

Christopher L. Parks - Boonton NJ, US
Susan E. Witko - New City NY, US
Maninder K. Sidhu - New City NY, US
J. Erik Johnson - Verona NJ, US
Roger Michael Hendry - Atlanta GA, US

Assignee:

Wyeth - Madison NJ

International Classification:

A61K 39/12
C12N 15/09
C12N 15/87
C12N 15/63

US Classification:

4241991, 435455, 435461, 4353201

Abstract:

A method of producing propagation-defective Vesicular Stomatitis Virus (VSV) in a cell culture is provided. The method involves introducing a plasmid vector encoding an optimized VSV G gene into a cell; expressing VSV G protein from the optimized VSV G gene; and introducing a propagation-defective VSV into the cell expressing the VSV G protein encoded by the optimized VSV G gene. The method further includes growing the cells in culture; and recovering the propagation-defective VSV from the culture.

US Patent:

20090175906, Jul 9, 2009

Filed:

Dec 18, 2008

Appl. No.:

12/338367

Inventors:

Narender Kumar Kalyan - Ridgewood NJ, US
Irina Yurgelonis - Ridgewood NJ, US
Roger Michael Hendry - Atlanta GA, US
Mark William Cutler - Bloomingdale NJ, US
Kristen Elissa Syvertsen - Tarrytown NY, US

Assignee:

Wyeth - Madison NJ

International Classification:

A61K 39/12
C12N 7/01
C12N 7/08

US Classification:

4242051, 4352351, 435237

Abstract:

The present invention relates to methods for generating genetically modified and attenuated strains of vesicular stomatitis virus (VSV) for use in the preparation of immunogenic compositions. More particularly, the invention relates to the identification of particular genetic modifications of attenuated VSV that result in an increased yield of virus and an increase in stability of the attenuated strains for preparation of the immunogenic compositions. Methods for cell culture propagation and use in large scale production of VSV is also disclosed.