Romualdas Vaisvila from 100 Central St #8, Ipswich, MA 01938, age 65, Phone: 9783567049

Repair Of Nucleic Acids For Improved Amplification
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US Patent:

8158388, Apr 17, 2012
Filed:

Apr 11, 2006
Appl. No.:

11/401826
Inventors:

Thomas C. Evans - Topsfield MA, US
Lixin Chen - Beverly MA, US
Chudi Guan - Wenham MA, US
Rebecca Kucera - Hamilton MA, US
Barton Slatko - Ipswich MA, US
Romualdas Vaisvila - Ipswich MA, US
Assignee:

New England Biolabs, Inc. - Ipswich MA
International Classification:

C12P 19/34
US Classification:

435 912
Abstract:

Methods and compositions are provided for repairing a polynucleotide so that it can be copied with improved fidelity and/or yield in, for example, an amplification reaction. This involves the use of a reaction mixture that includes a ligase and a cofactor selected from NAD or ATP and incubating the polynucleotide with the reaction mixture in the absence of Endonuclease VI. The reaction mixture may further contain an AP endonuclease and a polymerase. If used, these enzymes may be selected according to their ability to withstand high temperatures. For example, the reaction mixture may be used prior to a polynucleotide synthesis reaction in which case enzymes that are not thermophilic may be used. The repair reaction is not time sensitive with respect to seconds, minutes or hours of incubation in the enzyme mixture in as much as the repair is effected rapidly and prolonged incubation is not generally adverse.

Method For Cloning And Producing The Msei Restriction Endonuclease
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US Patent:

20050158769, Jul 21, 2005
Filed:

Dec 21, 2004
Appl. No.:

11/018116
Inventors:

Romualdas Vaisvila - Ipswich MA, US
Richard Morgan - Middleton MA, US
Rebecca Kucera - Hamilton MA, US
Elisabeth Raleigh - Somerville MA, US
Assignee:

New England Biolabs, Inc. - Beverly MA
International Classification:

C12Q001/68
C07H021/04
C12N009/10
C12N009/22
C12N001/21
C12N015/74
US Classification:

435006000, 435069100, 435193000, 435199000, 435252300, 435320100, 536023200
Abstract:

A method for cloning restriction-modification system is provided whereby the target modification methylase is produced and confers full protection during all growth phases in which the cognate restriction enzyme is present. The method is employed in the cloning of the MseI restriction-modification system.

Detection And Quantification Of Hydroxymethylated Nucleotides In A Polynucleotide Preparation
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US Patent:

20120156677, Jun 21, 2012
Filed:

Aug 25, 2010
Appl. No.:

13/392286
Inventors:

Jurate Bitinaite - Rowley MA, US
Romualdas Vaisvila - Ipswich MA, US
Sriharsa Pradhan - Wenham MA, US
Yu Zheng - Topsfield MA, US
Richard J. Roberts - Wenham MA, US
Christopher Noren - Boxford MA, US
Elisabeth A. Raleigh - Somerville MA, US
Geoffrey Wilson - South Hamilton MA, US
Assignee:

NEW ENGLAND BIOLABS, INC. - Ipswich MA
International Classification:

C12Q 1/68
US Classification:

435 611
Abstract:

Methods and compositions are described for detecting hydroxymethylated nucleotides (hmNs) in a polynucleotide preparation with a view to mapping the location of hmNs in a genome, quantifying the occurrence of hmNs at selected loci and correlating the occurrence of hmNs with gene expression and phenotypic traits. Embodiments describe the use of modifying enzymes together with site-specific endonucleases to detect the hmNs.

Method For Cloning And Producing The Msel Restriction Endonuclease
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US Patent:

6846658, Jan 25, 2005
Filed:

Oct 12, 2000
Appl. No.:

09/689343
Inventors:

Romualdas Vaisvila - Rockport MA, US
Richard D. Morgan - Middleton MA, US
Rebecca B. Kucera - Hamilton MA, US
Toby E. Claus - Beverly MA, US
Elisabeth A. Raleigh - Somerville MA, US
Assignee:

New England Biolabs, Inc. - Beverly MA
International Classification:

C12P 2100
C12N 121
C12N 1570
US Classification:

435183, 4353201, 435243, 4352521, 4352523, 536 231, 536 232, 536 237
Abstract:

A method for cloning restriction-modification system is provided whereby the target modification methylase is produced and confers full protection during all growth phases in which the cognate restriction enzyme is present. The method is employed in the cloning of the MseI restriction-modification system.

Detection And Quantification Of Hydroxymethylated Nucleotides In A Polynucleotide Preparation
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US Patent:

20150240310, Aug 27, 2015
Filed:

Apr 14, 2015
Appl. No.:

14/685966
Inventors:

- Ipswich MA, US
Romualdas Vaisvila - Ipswich MA, US
Sriharsa Pradhan - Wenham MA, US
Yu Zheng - Topsfield MA, US
Richard J. Roberts - Wenham MA, US
Christopher Noren - Boxford MA, US
Elisabeth A. Raleigh - Somerville MA, US
Geoffrey Wilson - South Hamilton MA, US
Assignee:

NEW ENGLAND BIOLABS, INC. - Ipswich MA
International Classification:

C12Q 1/68
Abstract:

Methods and compositions are described for detecting hydroxymethylated nucleotides (hmNs) in a polynucleotide preparation with a view to mapping the location of hmNs in a genome, quantifying the occurrence of hmNs at selected loci and correlating the occurrence of hmNs with gene expression and phenotypic traits. Embodiments describe the use of modifying enzymes together with site-specific endonucleases to detect the hmNs.

Compositions And Methods For Oxygenation Of Nucleic Acids Containing 5-Methylpyrimidine
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US Patent:

20150218530, Aug 6, 2015
Filed:

Apr 20, 2015
Appl. No.:

14/691088
Inventors:

- Ipswich MA, US
Lana Saleh - Hamilton MA, US
June Pais - Ipswich MA, US
Nan Dai - Gloucester MA, US
Richard J. Roberts - Wenham MA, US
Megumu Mabuchi - Beverly MA, US
Romualdas Vaisvila - Ipswich MA, US
Assignee:

NEW ENGLAND BIOLABS, INC. - Ipswich MA
International Classification:

C12N 9/02
C07K 14/47
C12Q 1/68
Abstract:

5-methylpyrimidine oxygenases and their use in the modification of nucleic acids are described.

Compositions And Methods For Oxygenation Of Nucleic Acids Containing 5-Methylpyrimidine
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US Patent:

20150132750, May 14, 2015
Filed:

Mar 14, 2013
Appl. No.:

13/827087
Inventors:

- Ipswich MA, US
Lana Saleh - Hamilton MA, US
June Pais - Ipswich MA, US
Nan Dai - Gloucester MA, US
Richard J. Roberts - Wenham MA, US
Megumu Mabuchi - Beverly MA, US
ROMUALDAS VAISVILA - IPSWICH MA, US
Assignee:

NEW ENGLAND BIOLABS, INC. - Ipswich MA
International Classification:

C12N 9/02
C12Q 1/68
US Classification:

435 611, 435189
Abstract:

5-methylpyrimidine oxygenases and their use in the modification of nucleic acids are described.

Romualdas Vaisvila