Ronald August Cook

US Patent:

6696298, Feb 24, 2004

Filed:

Dec 7, 2001

Appl. No.:

10/012729

Inventors:

Ronald M. Cook - Novato CA
Rand Dill - Corte Madera CA
Arnie Baltzley - San Anselmo CA

Assignee:

Biosearch Technologies, Inc. - Novato CA

International Classification:

G01N 110

US Classification:

436 54, 436 43, 436 48, 436180, 422100, 422151, 7386331, 2221453

Abstract:

Embodiments of the present invention are directed to an improved chemical synthesis apparatus for performing chemical synthesis such as nuclei acid synthesis in a plurality of reaction wells or cells in an efficient manner. The apparatus employs dispenser heads that each include a cluster of nozzles which are fluidicly coupled to a plurality of reagent sources for dispensing different reagents through the single dispenser head. Because each dispenser head is capable of dispensing a plurality of different reagents, the apparatus can perform simultaneous synthesis in a plurality of cells at a high throughput without complex and elaborate control of movement of the dispenser heads relative to the cells.

US Patent:

6761855, Jul 13, 2004

Filed:

Nov 5, 2001

Appl. No.:

10/011966

Inventors:

Ronald M. Cook - Novato CA
Rand Dill - Corte Madera CA

Assignee:

Biosearch Technologies, Inc. - Nevato CA

International Classification:

G01N 3060

US Classification:

422 70, 422 11, 422102, 73 6153, 2101982

Abstract:

The present invention is directed to an improved column for use in solid phase synthesis or purification. In a specific embodiment, the column is a disposable plastic column for use in solid phase synthesis or purification of complex chemicals, such as biomolecules and more specifically oligonucleotides. In some embodiments, the column has a top orifice with a sufficient diameter so that a fluid line or a multiple fluid line bundle may dispense fluids into the column with great efficiency. The column has an upper cavity portion configured and sized to render it compatible with dispensing pippetors, so that it can be used as a pipette tip or a pipettor can be used to aspirate the column. The column has a lower cavity portion with a shoulder for ready placement of a lower frit to contain the solid support in a central cavity portion of the column. An upper frit can be conveniently placed in the central cavity portion to seal the solid phase resin. The lower end tip of the column is configured as a luer-type fitting to provide a male luer connection.

US Patent:

7019129, Mar 28, 2006

Filed:

May 9, 2000

Appl. No.:

09/567863

Inventors:

Ronald M. Cook - Novato CA, US
Matt Lyttle - Point Reyes Station CA, US
Daren Dick - San Geronimo CA, US

Assignee:

Biosearch Technologies, Inc. - Novato CA

International Classification:

C07H 19/04
C12Q 1/68
C12M 3/00
C07K 1/00

US Classification:

536 266, 435 4, 435 5, 435 6, 4352872, 436501, 530350

Abstract:

The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.

US Patent:

7109312, Sep 19, 2006

Filed:

Jul 29, 2005

Appl. No.:

11/192705

Inventors:

Ronald M. Cook - Novato CA, US
Matt Lyttle - Point Reyes Station CA, US
Daren Dick - San Geronimo CA, US

Assignee:

Biosearch Technologies, Inc. - Novato CA

International Classification:

C07C 245/00
C07H 21/04
C07H 19/04

US Classification:

534558, 534563, 534604, 534606, 534610, 536 231, 536 266

Abstract:

The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.

US Patent:

7160996, Jan 9, 2007

Filed:

Jun 8, 2000

Appl. No.:

09/591185

Inventors:

Ronald M. Cook - Novato CA, US

Assignee:

Bioresearch Technologies, Inc. - Novato CA

International Classification:

C07H 21/04

US Classification:

536 243, 536 2431, 536 2432

Abstract:

The present invention provides a class of Conformationally Assisted Probes comprising (a) a nucleic acid moiety; (b) an energy donor moiety; (c) an energy acceptor moiety; and (d) one or more stabilizing moieties.

US Patent:

7582432, Sep 1, 2009

Filed:

May 19, 2006

Appl. No.:

11/437991

Inventors:

Ronald M. Cook - Novato CA, US
Matt Lyttle - Fairfax CA, US
Daren Dick - San Rafael CA, US

Assignee:

Biosearch Technologies, Inc. - Novato CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/00
C07H 21/02

US Classification:

435 6, 435 911, 435 912, 435 71, 435 72, 536 231, 536 261

Abstract:

The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.

US Patent:

7635598, Dec 22, 2009

Filed:

Jul 8, 2005

Appl. No.:

11/177998

Inventors:

Ronald M. Cook - Novato CA, US
Eliana Saxon Armstrong - Richmond CA, US
Hans E. Johansson - El Cerrito CA, US

Assignee:

Biosearch Technologies, Inc. - Novato CA

International Classification:

G01N 33/533
G01N 33/53
C12Q 1/26
C12N 1/21
C12P 21/00

US Classification:

436544, 436903, 436 56, 435 772, 435 25, 435 701, 4352523

Abstract:

In one aspect, the invention provides fluorescent probes and assays. The probes include a fluorophore-quencher pair that undergoes a switch from dark to fluorescent in response to a reaction of the quencher. The switch of the probe from dark to fluorescent is typically mediated by an enzyme that acts directly or indirectly on the quencher, interfering with its ability to quench fluorescence emission from the fluorophore. In another aspect, the invention provides a reporter gene assay system and methods of using this system. The assay system includes a fluorophore-quencher probe and an enzyme that acts directly or indirectly on the quencher, increasing the fluorescent emission of the fluorophore. In still other aspects, the invention provides nucleic acid constructs and cells expressing the peptide products of these constructs. In assays of the invention, the presence of a target substance is detected by the switching of fluorescence mediated by the change in oxidation state of the quencher.

US Patent:

7635762, Dec 22, 2009

Filed:

Oct 21, 2003

Appl. No.:

10/690806

Inventors:

Ronald M. Cook - Novato CA, US
Matt Lyttle - Point Reyes Station CA, US
Mary Katherine Johansson - El Cerrito CA, US

Assignee:

Biosearch Technologies, Inc. - Novato CA

International Classification:

C07H 19/00
C07H 21/00

US Classification:

536 221, 536 171, 536 185, 536 186

Abstract:

The present invention provides luminescent metal ion complexes for use in a wide range of biological and chemical studies. The luminescent metal ion complexes of the invention comprise a metal ion chelating component covalently bound to a carrier molecule. Also provided are methods of making and using the luminescent metal ion complexes.